Agilent 2200 TapeStation System User Manual

1 Agilent TechnologiesAgilent 2200 TapeStation SystemUser ManualAgilent 2200 TapeStation System - User ManualNotices Agilent Technologies, Inc. 2011-2014, 2015No part of this Manual may be reproduced in any form or by any means (including elec-tronic storage and retrieval or translation into a foreign language) without prior agree-ment and written consent from Agilent Technologies, Inc. as governed by United States and international copyright Part NumberG2964-90003 Rev. CEdition09/2015 Printed in GermanyAgilent TechnologiesHewlett-Packard-Strasse 8 76337 WaldbronnWarrantyThe material contained in this docu-ment is provided as is, and is sub-ject to being changed, without notice, in future editions.

2 Further, to the max-imum extent permitted by applicable law, Agilent disclaims all warranties, either express or implied, with regard to this Manual and any information contained herein, including but not limited to the implied warranties of merchantability and fitness for a par-ticular purpose. Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing, use, or perfor-mance of this document or of any information contained herein. Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms, the warranty terms in the sep-arate agreement shall Licenses The hardware and/or software described in this document are furnished under a license and may be used or copied only in accor-dance with the terms of such Rights LegendIf software is for use in the performance of a Government prime contract or subcon-tract.

3 Software is delivered and licensed as Commercial computer software as defined in DFAR (June 1995), or as a commercial item as defined in FAR (a) or as Restricted computer soft-ware as defined in FAR (June 1987) or any equivalent agency regulation or contract clause. Use, duplication or dis-closure of Software is subject to Agilent Technologies standard commercial license terms, and non-DOD Departments and Agencies of the Government will receive no greater than Restricted Rights as defined in FAR (c)(1-2) (June 1987). Government users will receive no greater than Limited Rights as defined in FAR (June 1987) or DFAR (b)(2) (November 1995), as applicable in any technical NoticesCAUTIONA CAUTION notice denotes a hazard.

4 It calls attention to an operating procedure, practice, or the like that, if not correctly per-formed or adhered to, could result in damage to the product or loss of important data. Do not proceed beyond a CAUTION notice until the indicated condi-tions are fully understood and met. WARNINGA WARNING notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in personal injury or death. Do not proceed beyond a WARNING notice until the indi-cated conditions are fully under-stood and 2200 TapeStation System - User Manual3 ContentsContents1 Introduction to the 2200 TapeStation System5 Overview of the System62 Specifications11 Technical Specifications12 ScreenTape Specifications143 Installing the System23 Unpacking the System24 Contents of the 2200 TapeStation System26 Installing the System304 Using the 2200 TapeStation System33 Intended Use of the 2200 TapeStation System35 Performance Limitations of Use36 Additional Components Required by the

5 User37 Operating Procedure38 How to prepare your samples50 Good Measurement Practices for Analysing DNA on the Agilent 2200 Ta p e S t a t i o n52 DNA Sample Preparation53 RNA Sample Preparation61 Protein Sample Preparation675 Maintenance71 General Information72 Changing the Needle73 Preventative Maintenance Interval76Ta p e S t a t i o n Te s t Ta p e784 Agilent 2200 TapeStation System - User ManualContents6 Appendix83 Limited Use Label License84 Sound Emission85 Waste Electrical and Electronic Equipment Directive86 Technical Service875 Agilent 2200 TapeStation System - User ManualAgilent Technologies1 Introduction to the 2200 TapeStation SystemOverview of the System6 ScreenTape Device Architecture7 Agilent 2200 TapeStation Instrument Components8 This chapter gives an introduction to the system6 Agilent 2200 TapeStation System - User Manual1 Introduction to the 2200 TapeStation SystemOverview of the SystemOverview of the SystemThe Agilent 2200 TapeStation System is a revolutionary automated platform for simpler, faster and more reliable electrophoresis.

6 It is made up of three elements: a consumable tape (ScreenTape device), an instrument (the 2200 TapeStation instrument), and an Agilent Software package (2200 TapeStation Controller Software, and Agilent TapeStation Analysis Software). The System is very straightforward to use, simply place the sample tubes and ScreenTape device in to the 2200 TapeStation instrument and let it load, separate, image, analyze, and present the User Manual guides the operation of all ScreenTape assays, the 2200 TapeStation instrument, and software for the analysis of DNA, RNA, and protein samples.

7 The contents of the ScreenTape System are detailed pertaining to the 2200 TapeStation System can be found in: Technical Specification Agilent 2200 TapeStation Instrument Components Installing the SystemPlace ScreenTape device and some tips in the 2200 TapeStation your samples in the 2200 TapeStation instrument and click Start on the 2200 TapeStation Controller your analyzed results in around 1 min per 2200 TapeStation System - User Manual7 Introduction to the 2200 TapeStation System1 Overview of the SystemInformation pertaining to sample and ScreenTape device requirements can be found in.

8 ScreenTape Device Architecture Operating ProcedureScreenTape Device ArchitectureFigure 1 Example: P200 ScreenTape deviceBarcode:The unique barcode tracks lane usage within each ScreenTape device and allows traceability of chamber:The buffer chamber is located at the top of the channel and contains optimised buffers for the effective separation of nucleic acid fragments or :The integrated electrodes apply a current across the ScreenTape device and eliminate the need for any additional electrophoresis :The gel contained within the ScreenTape device has been developed specifically to resolve nucleic acids or device product details:The information is unique to each ScreenTape device.

9 This includes: ScreenTape assay type, product expiry date and a unique serial 2200 TapeStation System - User Manual1 Introduction to the 2200 TapeStation SystemOverview of the SystemAgilent 2200 TapeStation Instrument ComponentsLid:The 2200 TapeStation instrument lid must be closed each time the 2200 TapeStation Controller Software is initialized, and whilst the instrument is in :The LED will illuminate once the instrument is on. When the LED is flashing slowly, the instrument is in use and the lid should not be opened, rapid flashing indicates that the 2200 TapeStation instrument requires Block:There are 2 sample blocks provided that can either hold mL sample tube strips or a 96-well Holder:The tip holder can accommodate up to 16 loading tips at any one device:The ScreenTape device must be placed into the holder with the barcode towards the front of the instrument, facing towards the Socket.

10 The USB connector is inserted into the USB socket to link the laptop to the 2200 TapeStation socket:The power cable must be connected to the 2200 TapeStation instrument and the relevant mains electricity 2200 TapeStation System - User Manual9 Introduction to the 2200 TapeStation System1 Overview of the SystemFigure 22200 TapeStation instrument (front view)Figure 32200 TapeStation instrument (back view)A^YHXgZZcIVeZ YZk^XZI^e ]daYZgA:9 HVbeaZ WadX`USB socketOn/off switchPower-cable socket10 Agilent 2200 TapeStation System - User Manual1 Introduction to the 2200 TapeStation SystemOverview of the System11 Agilent 2200 TapeStation System - User ManualAgilent Technologies2 Specifications Technical Specifications12 ScreenTape Specifications14 This chapter provides information on 2200 TapeStation System - User Manual2 SpecificationsTechnical SpecificationsTechnical Specifications2200 TapeStation instrumentInput voltage:12 V DCPower consumption:40 W (VA)Current:3 AInterface:USB cable (PC comms.)