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Agilent 6460/6420 Triple Quad Mass Spectrometer (Model ...

Agilent TechnologiesAgilent 6460/6420 Triple quad mass Spectrometer ( model k6460 / k6420 ) SystemConcepts GuideThe Big PictureFor In Vitro Diagnostic Use2 Agilent k6460 / k6420 Triple quad mass Spectrometer Concepts GuideNotices Agilent Technologies, Inc. 2017No part of this manual may be reproduced in any form or by any means (including elec-tronic storage and retrieval or translation into a foreign language) without prior agree-ment and written consent from Agilent Technologies, Inc. as governed by United States and international copyright Part NumberK3335-90206 EditionRevision A, February 2017 Printed in USAA gilent Technologies, Inc. 5301 Stevens Creek Blvd. Santa Clara, CA 95051 WarrantyThe material contained in this docu-ment is provided as is, and is sub-ject to being changed, without notice, in future editions. Further, to the max-imum extent permitted by applicable law, Agilent disclaims all warranties, either express or implied, with regard to this manual and any information contained herein, including but not limited to the implied warranties of merchantability and fitness for a par-ticular purpose.

Agilent Technologies Agilent 6460/6420 Triple Quad Mass Spectrometer (Model K6460/K6420) System Concepts Guide The Big Picture For In Vitro Diagnostic Use

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Transcription of Agilent 6460/6420 Triple Quad Mass Spectrometer (Model ...

1 Agilent TechnologiesAgilent 6460/6420 Triple quad mass Spectrometer ( model k6460 / k6420 ) SystemConcepts GuideThe Big PictureFor In Vitro Diagnostic Use2 Agilent k6460 / k6420 Triple quad mass Spectrometer Concepts GuideNotices Agilent Technologies, Inc. 2017No part of this manual may be reproduced in any form or by any means (including elec-tronic storage and retrieval or translation into a foreign language) without prior agree-ment and written consent from Agilent Technologies, Inc. as governed by United States and international copyright Part NumberK3335-90206 EditionRevision A, February 2017 Printed in USAA gilent Technologies, Inc. 5301 Stevens Creek Blvd. Santa Clara, CA 95051 WarrantyThe material contained in this docu-ment is provided as is, and is sub-ject to being changed, without notice, in future editions. Further, to the max-imum extent permitted by applicable law, Agilent disclaims all warranties, either express or implied, with regard to this manual and any information contained herein, including but not limited to the implied warranties of merchantability and fitness for a par-ticular purpose.

2 Agilent shall not be liable for errors or for incidental or consequential damages in connec-tion with the furnishing, use, or per-formance of this document or of any information contained herein. Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms, the warranty terms in the sep-arate agreement shall Licenses The hardware and/or software described in this document are furnished under a license and may be used or copied only in accor-dance with the terms of such Rights Government Restricted Rights. Soft-ware and technical data rights granted to the federal government include only those rights customarily provided to end user cus-tomers. Agilent provides this customary commercial license in Software and techni-cal data pursuant to FAR (Technical Data) and (Computer Software) and, for the Department of Defense, DFARS (Technical Data - Commercial Items) and DFARS (Rights in Commercial Computer Software or Com-puter Software Documentation).

3 Safety NoticesCAUTIONA CAUTION notice denotes a haz-ard. It calls attention to an operat-ing procedure, practice, or the like that, if not correctly performed or adhered to, could result in damage to the product or loss of important data. Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met. WARNINGA WARNING notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly per-formed or adhered to, could result in personal injury or death. Do not proceed beyond a WARNING notice until the indicated condi-tions are fully understood and RevisionThis guide applies to the Agilent MassHunter Workstation Software -- Data Acquisition for 6460 Triple Quadrupole LC/MS program version to CustomerThe k6460 / k6420 mass Spectrometer is intended to be used to identify inorganic or organic compounds in human specimens by ionizing the compounds and separating the resulting ions by means of electrical field according to their ManufacturingManufactured by Agilent Technologies Singapore Pte.

4 Ltd. No. 1 Yishun Ave-nue 7, Singapore 768923 Operating Temperature: 15 C to 35 C Storage Temperature: -40 C to 70 CAgilent k6460 / k6420 Triple quad mass Spectrometer Concepts Guide3In This Concepts Guide presents The Big Picture behind the operation of the k6460 / k6420 Triple quad mass Spectrometer by helping you understand how the hardware and software Overview Learn how the k6460 / k6420 Triple quad mass Spectrometer helps you do your Inner Workings Learn how the k6460 / k6420 Triple quad mass Spectrometer helps you do your k6460 / k6420 Triple quad mass Spectrometer and SensitivityLearn how the k6460 / k6420 Triple quad mass Spectrometer achieves high Agilent MassHunter Workstation Software - Data Acquisition for 6400 Series Triple QuadrupoleLearn concepts behind the design of the Agilent MassHunter Workstation Software - Data Acquisition for 6400 Series Triple Quadrupole mass Spectrometer . 4 Agilent k6460 / k6420 Triple quad mass Spectrometer Concepts GuideThis page intentionally left k6460 / k6420 Triple quad mass Spectrometer Concepts Guide5 Content1 Overview 7 Supported configuration 8 Help for acquisition 9 Help for data analysis 112 Inner Workings 13 Design of the k6460 / k6420 Triple quad mass Spectrometer 13 Innovative Enhancements in the k6460 15 Innovative Enhancements in the k6420 16 How a Triple quadrupole mass Spectrometer works 17 How Dynamic MRM works 19 How Triggered Dynamic MRM works 223K6460/ k6420 Triple quad mass Spectrometer and Sensitivity 29 How the k6460 / k6420 Triple quad mass Spectrometer improves sensitivity 30 Noise reduction 30 Example of chemical noise reduction 32 How each component works to improve sensitivity 34 mass Spectrometer Ion Sources 34 Front-end ion optics 38 Collision cell 40 Detector 44

5 Pumping system 456 Agilent k6460 / k6420 Triple quad mass Spectrometer Concepts Guide4 Agilent MassHunter Workstation Software - Data Acquisition for 6400 Series Triple Quadrupole 47 Tuning 49 Acquisition 527 Agilent TechnologiesK6460/ k6420 Triple quad mass SpectrometerConcepts Guide1 OverviewSupported configuration 8 Help for acquisition 9 Help for data analysis 11 This chapter provides an overview of the k6460 / k6420 Triple quad mass Spectrometer components and how they help get the job done. 8 Agilent k6460 / k6420 Triple quad mass Spectrometer Concepts Guide1 Overview Supported configuration Supported configurationESI Electrospray Ionization APCI Atmospheric Pressure Chemical Ionization AJS Agilent Jet Stream Technology The k6460 / k6420 Triple quad mass Spectrometer can be used with a K1260 LC stack. The supported ion sources are AJS, ESI, and AJS source uses a super-heated sheath gas to collimate the nebulizer spray, which dramatically increases the number of ions that enter the mass Help for acquisitionAgilent k6460 / k6420 Triple quad mass Spectrometer Concepts Guide9 Help for acquisitionTo help you use the k6460 / k6420 Triple quad mass Spectrometer for these applications, the software lets you do these tasks in a single window with the Data Acquisition Program:Prepare the instrumentTo learn how to install the Triple quad mass Spectrometer , see the Installation Guide.

6 To learn how to get started with the Triple quad mass Spectrometer , see the Quick Start Guide. To learn more about how to use the Agilent Triple Quadrupole LC/MS with real samples and data, see the Familiarization Guide To learn how to do individual tasks with the Triple quad mass Spectrometer , see the online Help. To learn more about an Agilent 1260 Infinity LC module, see the Agilent 1260 Infinity LC User s Guide for the module. Start and stop the instruments from the software. Download settings to the Agilent 1260 Infinity LC and the k6460 / k6420 Triple quad mass Spectrometer in real time to control the instrument. Evaluate if the MS parameters are within the limits to produce the specified mass accuracy and resolution with a Checktune report. Optimize MS parameters automatically (Autotune) or manually through Agilent tuning programs and print an Autotune report. Monitor the actual conditions of the instrument View the real-time plot for chromatograms and instrument parameters (both UV/Vis and MS) and print a real-time plot report.

7 View the centroided line spectrum of a peak or the mass range profile spectrum of a peak in real learn about the Skyline software program and the Triple quad mass Spectrometer , see the Peptide Quantitation with Skyline Workflow up acquisition methods Enter and save parameter values for all LC modules and the LC/MS to an acquisition method. Select and label the total ion chromatograms or extracted ion chromatograms that you want to appear in the real-time plot. Set up time segments for each scan type and analysis where parameters change with the time segment or with the scans within the time k6460 / k6420 Triple quad mass Spectrometer Concepts Guide1 Overview Help for acquisition Print an acquisition method report. Optimizer software enables automated determination of compound MRM parameters, including fragmentor voltage and collision data Enter sample information and pre- or post-analysis programs (scripts) and run single samples worklist is a list of individual samples and batches (sequences) that you enter and run automatically with the Data Acquisition program.

8 Enter and automatically run both individual samples and samples organized in a worklist (sequence of samples). Set up pre- and post-analysis scripts to run between samples in a worklist. Set up and run a worklist to optimize LC/MS acquisition parameters. Print a worklist report. View system events, including start and stop times, run events and errors and print an event log Manager program Create a study to group together a collection of samples and operations. Run one or more studies automatically. Run Quantitative Analysis on the results automatically. Review the studies that have already been run. Create a study from an existing worklist. Create a study from a text file which can be in several different formats. Create a study from a spreadsheet to run Drug Discovery Screening. Create a study to optimize MS parameters (such as Fragmentor Voltage or Collision Energy).Overview1 Help for data analysisAgilent k6460 / k6420 Triple quad mass Spectrometer Concepts Guide11 Help for data analysisQuantitative Analysis ProgramAgilent designed the quantitative analysis program to help quantitate very low amounts of material with the following unique features: Imports information directly from the acquisition method.

9 Provides a curve-fit assistant to test all fits and statistics on curve quality. Integrates with an automated, parameter-free integrator that uses a novel algorithm, optimized for Triple quadrupole data. Presents a Batch-at-a-Glance results window to help you review and operate on an entire batch of data at once. Automatically detects outliers. Provides preconfigured templates for basic reporting and enables the capability to create custom reports in Microsoft ExcelPlease refer to the Agilent MassHunter Workstation Software - Quantitative Analysis Familiarization Guide or the online Help for the Quantitative Analysis Analysis ProgramFor fast method development, this software is used to quickly review the qualitative aspects of the data, such as the optimum precursor to product ion designed the Qualitative Analysis program to present large amounts of data for review in one central location. With the program you can do these operations for any type of mass Spectrometer data that you open: Extract chromatograms View and extract peak spectra Subtract background Integrate the chromatogram12 Agilent k6460 / k6420 Triple quad mass Spectrometer Concepts Guide1 Overview Help for data analysis Find compounds Identify compounds Export resultsYou can also set up methods to automatically do the tasks in the list, as well as others, when you open the data refer to the Agilent MassHunter Workstation Software - Qualitative Analysis Familiarization Guide or the online Help for the Qualitative Analysis ProgramThe Skyline software package (from MacCoss Lab at the University of Washington) provides an intuitive set of peptide-centric tools.

10 Among its many features, Skyline can create Agilent QQQ acquisition methods to run targeted Proteomics experiments. Skyline can also create transition lists. This software is open source and is available for you to install on your computer at no refer to the Agilent Triple Quadrupole LC/MS Peptide Quantitation with Skyline Workflow Guide (p/n 5990-9887EN) and Overview (p/n 5990-9886EN) for more TechnologiesK6460/ k6420 Triple quad mass SpectrometerConcepts Guide2 Inner WorkingsDesign of the k6460 / k6420 Triple quad mass Spectrometer 13 How a Triple quadrupole mass Spectrometer works 17 Innovative Enhancements in the k6460 15 How Dynamic MRM works 19 How Triggered Dynamic MRM works 22In this chapter you learn about concepts to help you understand the inner workings of the k6460 / k6420 Triple quad mass of the k6460 / k6420 Triple quad mass SpectrometerThe Triple quadrupole mass Spectrometer consists of an ion source, enhanced desolvation technology, followed by ion optics that transfer the ions to the first quadrupole positioned to the right of it.


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