실험목적 - amborella.net

1 , , , vortex mixer DNA Basic typeP2 white tipP10 white tipP20 yellow tipP100 yellow tipP200 yellow tipP1000 blue tipMulti-channel pipetteAuto-pipettesElectric Balance g value: gravity rpm: round per minutes Rotor: swing bucket :- .- blank tube .. Vortex Mixer Touch MixerFume hood (electrophoresis)- : DNA RNA, protein gel.

2 -Agarose gel: DNA , PCR product EtBr ( ) 1~ agarose gel . - (buffer): buffer 1X TAE (Tris base, Acetic acid, EDTA) 1X TBE (Tris base, Boric acid, EDTA) , 10X 1X . X , 10X 10 . -DNA : : DNA - + .- : DW (distilled water) TE (Tris-EDTA) DNA , buffer . DNA loading dye loading.

3 Loading dye bromophenolblue DNA , sucrose DNA buffer well . DNA - DNA . EtBr (Ethidium bromide) DNA . - EtBr DNA DNA . - EtBr (ultra-violet ray) . - DNA EtBr agarose gel , DNA EtBr EtBr DNA.

4 - DNA . EtBr DNA intercalating agent (frame-shift mutation) .. EtBr , . EtBr EtBr . EtBr DNA . agarose gel - Agarose gel cast cassette comb - 250ml 1) 40ml 1X TAE buffer2) agarose vinyl wrap 2~3 - 1 - 30 agarose ( 15 ).

5 - Vinyl wrap ( ) EtBr (Goodview) 4ul ..- cast . Gel 7mm . pipette tip ( cassette . Comb comb comb ).- 10~20 gel . Gel (1 ) vinyl wrap gel . : , agarose, 1 XTAE buffer, , , EtBr (Goodview TM), , parafilm, UV gel-documentation system 1.

6 DNA agarose TAE/TBE buffer . ( ; agarose )2. agarose EtBr (Goodview) . 3. gel plate , comb gel . 4. DNA size marker gel loading dye sample parafilm well . 5. 50-100V 15-30 . 6. UV transilluminator agarose gel . ( , EtBr agarose gel .)UV UV protector UV gel.

7 DNA : DNA , , DNA , , .Lamda phage DNA - Lamda phage serial dilution DNA DNA .-Serial dilutionLamda DNA DW 1/10 3ul27ul 1/50 3ul12ul 1/250 3ul12ul-Loading 1) Parafillim loading dye ( ) loading 1ul .2) 1 lane ladder 3ul loading3) 2~4 lane 5ul 1ul loading dye pipette ( ) 6ul loading DNA - DNA spectrophotometer.

8 DNA 260nm 260nm OD (optical density) . DNA . - 280nm OD 260/280 DNA .- spectrophotometer (cuvette 1ml~100ul ) , OD DNA 1/10~1/100 .- spectrophotometer DNA .- spectrophotometer Nanodrop 1ul DNA .UV-visible SpectrophotometerNano drop spectrophotometer Background 1 ( ).

9 1. DNA .2. DNA DNA.