Antibodies to Human Cell-Surface Markers Tested for BD ...

1 Unless otherwise specified, all products are for Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for resale. Prices are subject to change without notice. All applications are either Tested in-house or reported in the literature. See Technical Data Sheets for to Human Cell-Surface Markers Tested for BD Phosflow ProtocolsMany Cell-Surface antigens are sensitive to fixation/permeabilization procedures, resulting in loss of recognition by their respective Antibodies . For your information, BD Biosciences has Tested many Antibodies conjugated to various fluorochromes under several fixation/permeabilization conditions, and the results are summarized as IDetergent methodProtocol IIMild alcohol methodProtocol IIIH arsh alcohol methodProtocol IVDetergent methodFix buffer recommended for PBMCs or cell lines:BD Cytofix Buffer (554655)BD Cytofix Buffer (554655)BD Cytofix Buffer (554655)BD Cytofix Buffer (554655)Fix buffer recommended for whole blood, splenocytes, bone marrows, and other sample types containing erythrocytes:BD Phosflow Lyse/Fix Buffer (558049)BD Phosflow Lyse/Fix Buffer (558049)BD Phosflow Lyse/Fix Buffer (558049)BD Phosflow Lyse/Fix Buffer (558049)Perm Buffer.

2 BD Phosflow Perm/Wash Buffer I (557885)BD Phosflow Perm Buffer II (558052)BD Phosflow Perm Buffer III (558050)BD Phosflow Perm Buffer IV (560746)SpecificityCloneFluorochromeHuma n CD3SK7 APC++++APC-Cy 7++ +FITC++++PE++++PE-Cy 7++++PerCP+++/ PerCP-Cy ++++UCHT1 Alexa Fluor 488++++Alexa Fluor 647++++Alexa Fluor 700++/ APC++++FITC++++BD Horizon V450++Pacific Blue + + + PE++++PE-Cy 5++++PE-Cy7++++HIT3aAPC+ +/ FITC PE+ PE-Cy5+ SP34PE-Cy7+ +/ PerCP+++ Human CD4L200 FITC+++PE+++ PE-Cy7+++ PerCP+++ +++RPA-T4 Alexa Fluor 488+++ Alexa Fluor 647+++ Alexa Fluor 700+ APC++++APC-Cy7+ FITC+++ Pacific Blue +++ PE++++PE-Cy5++ PE-Cy7+++ SK3 AmCyan APC++++APC-Cy7+/ FITC++++PE++++PE-Cy7+++PerCP+++ ++++L120PE Human CD5 UCHT2PE+++APC++/ Notes.

3 Blank indicates not Tested + Good resolution between positive and negative peaks- Recommended Poor resolution between positive and negative peaks - Not Recommeded+/ Donor dependent. The positive and negative peaks are not separated well for some donorsUS Orders: otherwise specified, all products are for Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for resale. Prices are subject to change without notice. All applications are either Tested in-house or reported in the literature. See Technical Data Sheets for to Human Cell-Surface Markers Tested for BD Phosflow Protocols (continued)Notes:Blank indicates not Tested + Good resolution between positive and negative peaks- Recommended Poor resolution between positive and negative peaks - Not Recommeded+/ Donor dependent.

4 The positive and negative peaks are not separated well for some donorsProtocol IDetergent methodProtocol IIMild alcohol methodProtocol IIIH arsh alcohol methodProtocol IVDetergent methodFix buffer recommended for PBMCs or cell lines:BD Cytofix Buffer (554655)BD Cytofix Buffer (554655)BD Cytofix Buffer (554655)BD Cytofix Buffer (554655)Fix buffer recommended for whole blood, splenocytes, bone marrows, and other sample types containing erythrocytes:BD Phosflow Lyse/Fix Buffer (558049)BD Phosflow Lyse/Fix Buffer (558049)BD Phosflow Lyse/Fix Buffer (558049)BD Phosflow Lyse/Fix Buffer (558049)Perm Buffer:BD Phosflow Perm/Wash Buffer I (557885)BD Phosflow Perm Buffer II (558052)BD Phosflow Perm Buffer III (558050)BD Phosflow Perm Buffer IV (560746)

5 SpecificityCloneFluorochromeHuman CD8 HIT8aAPC-Cy7++ +FITC++++PE++++PE-Cy5+++RPA-T8 Alexa Fluor 488++++Alexa Fluor 647++++Alexa Fluor 700+APC++++APC-Cy7+FITC++++Pacific Blue ++PE++++PE-Cy5++PE-Cy5++++PE-Cy7++ +SK1 APC++++APC-Cy7+/ APC-H7+ FITC++++/ PE+++PE-Cy7+ PerCP+/ +/ +++ + Human CD11a/LFA-1G43-25 BPE++/ Hi111 APC+ PE++/ Human CD11bICRF44 Alexa Fluor 488+ APC+ APC-Cy7 FITC PE++/ +/ PE-Cy5+ PE-Cy7+ Human CD11cBLy6 APC++/ +/ PE++/ +/ Human CD13WM-15PE+/ +/ Human CD14M5E2 Alexa Fluor 488++/ +Alexa Fluor 700 APC++/ +FITC++/ PE++/ +PE-Cy7+ + + +MOP9PE+ APC+ PerCP+ APC-Cy7+ Human CD15W6D3PE++HI98PE++US Orders: otherwise specified, all products are for Research Use Only. Not for use in diagnostic or therapeutic procedures.

6 Not for resale. Prices are subject to change without notice. All applications are either Tested in-house or reported in the literature. See Technical Data Sheets for to Human Cell-Surface Markers Tested for BD Phosflow Protocols (continued)Notes:Blank indicates not Tested + Good resolution between positive and negative peaks- Recommended Poor resolution between positive and negative peaks - Not Recommeded+/ Donor dependent. The positive and negative peaks are not separated well for some donorsProtocol IDetergent methodProtocol IIMild alcohol methodProtocol IIIH arsh alcohol methodProtocol IVDetergent methodFix buffer recommended for PBMCs or cell lines:BD Cytofix Buffer (554655)BD Cytofix Buffer (554655)BD Cytofix Buffer (554655)BD Cytofix Buffer (554655)Fix buffer recommended for whole blood, splenocytes, bone marrows, and other sample types containing erythrocytes:BD Phosflow Lyse/Fix Buffer (558049)BD Phosflow Lyse/Fix Buffer (558049)BD Phosflow Lyse/Fix Buffer (558049)BD Phosflow Lyse/Fix Buffer (558049)Perm Buffer.

7 BD Phosflow Perm/Wash Buffer I (557885)BD Phosflow Perm Buffer II (558052)BD Phosflow Perm Buffer III (558050)BD Phosflow Perm Buffer IV (560746)SpecificityCloneFluorochromeHuma n CD163G8 Alexa Fluor 647 Alexa Fluor 700 APC-Cy7 FITC+/ +/ Pacific Blue PE++/ PE-Cy7+ ++++NKP15 FITC G022 Purified Human ++ +PE+++ Human CD19 HIB19 Alexa Fluor 488 Alexa Fluor 700 APC++/ FITC++/ PE++/ PE-Cy7 SJ25C1 AmCyan APC+/ +/ APC-Cy7 APC-H7 FITC PE++/ +/ +/ PE-Cy7+/ PerCP 4G7 FITC+/ PE PerCP Human CD202H7 APC+ FITC+/ PE+ PE-Cy5 L27 APC + APC-Cy7 FITC PE+ PE-Cy7+ PerCP Human CD20 (I/C)H1 (FB1)Alexa Fluor 488++++Alexa Fluor 647++++ ++++ Human CD23M-L233PE++/ Human CD25M-A251 Alexa Fluor 647++++APC++ FITC+++ PE++++PE-Cy7++ 2A3PE++++APC++++FITC++++PE-Cy7+ US Orders: otherwise specified, all products are for Research Use Only.

8 Not for use in diagnostic or therapeutic procedures. Not for resale. Prices are subject to change without notice. All applications are either Tested in-house or reported in the literature. See Technical Data Sheets for to Human Cell-Surface Markers Tested for BD Phosflow Protocols (continued)Notes:Blank indicates not Tested + Good resolution between positive and negative peaks- Recommended Poor resolution between positive and negative peaks - Not Recommeded+/ Donor dependent. The positive and negative peaks are not separated well for some donorsProtocol IDetergent methodProtocol IIMild alcohol methodProtocol IIIH arsh alcohol methodProtocol IVDetergent methodFix buffer recommended for PBMCs or cell lines:BD Cytofix Buffer (554655)BD Cytofix Buffer (554655)BD Cytofix Buffer (554655)BD Cytofix Buffer (554655)Fix buffer recommended for whole blood, splenocytes, bone marrows, and other sample types containing erythrocytes:BD Phosflow Lyse/Fix Buffer (558049)BD Phosflow Lyse/Fix Buffer (558049)BD Phosflow Lyse/Fix Buffer (558049)BD Phosflow Lyse/Fix Buffer (558049)Perm Buffer.

9 BD Phosflow Perm/Wash Buffer I (557885)BD Phosflow Perm Buffer II (558052)BD Phosflow Perm Buffer III (558050)BD Phosflow Perm Buffer IV (560746)SpecificityCloneFluorochromeHuma n CD27L128 APC+++FITC+++PE++++M-T271PE Human FITC PE Human CD31WM59PE++/ +/ Human CD33WM53PE++/ +/ +FITC PE++++PE-Cy7 Human CD348G12 APC+++/ FITC+++/ PE++++PE-Cy7++/ PerCP+++ +++ 581 APC++/ +/ FITC++++/ PE++++563PE++++ Human CD38HB7 FITC PE HIT2 APC PE Human CD405C3 APC FITC PE Human CD44515PE++G44-26 APC++++PE++ Human CD452D1 AmCyan++APC++APC-Cy7+++APC-H7+++FITC++Pe rCP++ ++HI30 APC+++FITC+++BD Horizon V450+++PE++++PE-Cy5++PE-Cy7+++ Human CD45RA5H9 FITC++++PE+++L48 FITC+++HI100 APC++++FITC++++BD Horizon V450+++PE++++PE-Cy5+++US Orders: otherwise specified, all products are for Research Use Only.

10 Not for use in diagnostic or therapeutic procedures. Not for resale. Prices are subject to change without notice. All applications are either Tested in-house or reported in the literature. See Technical Data Sheets for to Human Cell-Surface Markers Tested for BD Phosflow Protocols (continued)Notes:Blank indicates not Tested + Good resolution between positive and negative peaks- Recommended Poor resolution between positive and negative peaks - Not Recommeded+/ Donor dependent. The positive and negative peaks are not separated well for some donorsProtocol IDetergent methodProtocol IIMild alcohol methodProtocol IIIH arsh alcohol methodProtocol IVDetergent methodFix buffer recommended for PBMCs or cell lines:BD Cytofix Buffer (554655)BD Cytofix Buffer (554655)BD Cytofix Buffer (554655)BD Cytofix Buffer (554655)Fix buffer recommended for whole blood, splenocytes, bone marrows, and other sample types containing erythrocytes:BD Phosflow Lyse/Fix Buffer (558049)BD Phosflow Lyse/Fix Buffer (558049)BD Phosflow Lyse/Fix Buffer (558049)BD Phosflow Lyse/Fix Buffer (558049)Perm Buffer.