Antibody Purification - Harvard University

-Purity check and functional analysis ... Source-Sample preparation Introduction The diversity of the antibody-antigen interaction and our ability to manipulate the charac-teristics of the interaction has created many uses for antibodies and antibody fragments, ... Because of the phylogenetic distance between birds and mammals, IgY can be used ...

Fullscreen Download

Tags:

Analysis, Introduction, Phylogenetic

Information

Domain:

Source:

Link to this page:

Please notify us if you found a problem with this document:

Other abuse

Transcription of Antibody Purification - Harvard University

Documents from same domain

Phosphorylation Analysis by Mass Spectrometry

kirschner.med.harvard.edu

why a phosphorylation analysis using LC/MS failed. To test this we used a set of peptide/phosphopeptide pairs that var-ied in length from 7 to 17 amino acids, resembling the size of peptides commonly observed in tryptic digests. Cysteine and methionine-containing peptides were not included in this

Mass, Spectrometry, Mass spectrometry, Phosphorylation

User Guide - Harvard University

kirschner.med.harvard.edu

CENTRICON® Centrifugal Filter Devices User Guide Centricon centrifugal filter devices with 10,000 NMWL, 30,000 NMWL, 50,000 NMWL, and 100,000 NMWL Ultracel® YM membranes are approved for in-vitro diagnostic use. Centricon centrifugal filter devices with 3,000 NMWL Ultracel YM membranes are for research use only and are not for use in diagnostic

Guide, User, User guide, Centrifugal

Gel Filtration - Harvard University

kirschner.med.harvard.edu

Size Gel filtration (GF), also called size exclusion Charge Ion exchange chromatography (IEX) Hydrophobicity Hydrophobic i nteraction chromatography (HIC) Reversed phase chromatography (RPC) Biorecognition (ligand specificity) Affinity chromatography (AC) Gel filtration Hydrophobic interaction Ion exchange Affinity Reversed phase Fig. 1.

Size, Exclusion, Size exclusion

QIAGEN Plasmid Purification Handbook

kirschner.med.harvard.edu

Risk and safety phrases:* R36/38, S13-26-36-46 Buffers QBT, QC, QF Contain isopropanol: flammable. Risk and safety phrases:* R10 RNase A Contains ribonuclease: sensitizer. Risk and safety phrases:* R42/43, S23-24-26-36/37 24-hour emergency information Emergency medical information in English, French, and German can be obtained 24 hours a day from:

French, Phrases

Useful fundamental numbers in molecular biology

kirschner.med.harvard.edu

Yeast (Saccharomyces cerevisiae): ~5 mm diameter, ~50 mm3 in volume Mammalian (HELA) cell - ~2,000 mm3 in volume, adherent cell on a slide ~20 mm diameter Æ ~100,000 cells in a confluent well of a 96 multiwell plate Organelles and cell constituents: Mammalian cell nucleus ~10 micron diameter

Saccharomyces, Cerevisiae, Saccharomyces cerevisiae

Manual: QuikChange® Site-Directed Mutagenesis Kit

kirschner.med.harvard.edu

Belgium 00800 7000 7000 00800 7001 7001 00800 7400 7400 0800 15775 0800 15740 0800 15720 France 00800 7000 7000 00800 7001 7001 00800 7400 7400 ... (0.1 ng/μl in TE bufferc) 10 μl 10 μl a The QuikChange Site-Directed Mutagenesis Kit (Catalog #200518) contains enough reagents for 30 total reactions, which includes 5 control reactions.

7000, 7000 7000