API & ID 32

1 API & id 32 IDENTIFICATION DATABASESINTRODUCTIONThe API , id 32 and rapid id 32 database update takes into account: the evolution of international taxonomy the description of the new bacterial species, newly acquired bacteriology data (new profiles for bacterial strains which have an impact on performance data)As a result of the update, the APIWEB software version has changed from version to version API and id 32 databases have again been updatedTwenty-two of the twenty-three identification databases have been revised, taking account the biochemical profiles of over 56,277 , 697 species of bacteria and yeasts can be identified, including 14 new species and 50 that have been assigned new S CHANGED IN THE DATABASES?2 The changes made can be broken down as follows:A number of new species have been added to the database (including both entirely new species and others added on the basis of new results).

2 Certain bacterial species have been deleted due to more stringent criteria. Certain rare species which are not sufficiently studied have been removed from the names of certain species have been changed to follow modifications in the bacterial taxonomy as officially described in the International Journal of Systematic and Evolutionary have been revised to reflect the changes in names and the species added and and performances have been altered to reflect variations observed in the profiles analyzed as the database was tests were modified to reflect the new reference information S CHANGED IN THE DATABASES?DatabaseVersion numberChanges to thesaurusChanges to databaseoldnewTaxonsNotesIdentificationA dditional testsAPI 20 Ev 20 E v 10 Sv 20 NEv STAPHv 20 STREPv 20 C AUXv CANDIDAv 20 Av CORYNEv CAMPYv LISTERIAv NHv 50 CHBv 50 CHEv 50 CHLv 32 Ev id 32 Ev id 32 STAPHv id 32 STREPv 32 Cv id 32 Av NE10 S20 E50 CHERapiD 20 EID 32 ERapiD id 32 E GRAMBACILLIOXYDASEOXYDASE4 ORIENTATION TESTSGRAM BACILLIORIENTATION TESTSOTHER GRAM BACILLIS mall Gram bacilli (Haemophilus)

3 Growth with CO2 atmophereCurved bacilli,or in spiralGrowth with microaerophilic atmosphereAnerobesNeisseriaHaemophilusCa mpylobacterBecteroidesFusobacteriumPrevo tellaCAMPYNH20 ARapiD id 32 A GRAMBACILLIOXYDASE5 ORIENTATION TESTSOTHER GRAM BACILLI6 ORIENTATION TESTSGRAM BACILLIORIENTATION TESTSCOCCI AnaerobesSporulated bacilliSmall bacilliof characteristic shapePartial discoloring(granulation)Short bacilliwith rounded endsMobile at 20 - 25 CImmobile at 37 CLarge sporulatedbacilliVery taperedbacilliClostridium20 ARapiD id 32 ACoryneListeriaCHBC orynebacteriumListeriaBacillus50 CHLL actobacillusCATALASECATALASE GRAMBACILLIAero/Anaerobes7 ORIENTATION TESTSCOCCI 20 STREPR apid id 32 AID 32 STAPH20 ESTAPHNHCOCCIGRAMCATALASECATALASECOCCIGR AMCOCCI in pairsAnaerobesStaphylococcusMicrococcusR apid id 32 STREPS treptococcusEnterococcusVeillonellaNeiss eriaSPECIES IDENTIFIABLE BY THE VARIOUS IDENTIFICATION SYSTEMS8 ORIENTATION TESTSYEASTSC andidaCryptococcusGeotrichumKloeckeraPic hiaRhodoturelaTrichosporonAssimilation testsSugar acidification orenzymatic tests20C AUXID 32

4 CCandidaCryptococcusSaccharomycesTrichos poronCANDIDAMICROSCOPICOBSERVATIONYEASTS SPECIES IDENTIFIABLE BY THE VARIOUS IDENTIFICATION SYSTEMSAPI 20 EGram-negative bacilliAPI 10 SGram-negative bacilliRapid 20 ETME nterobacteriaceaeAPI 20 NEGram-negative non-EnterobacteriaceaeAPI StaphStaphylococciAPI 20 StrepStreptococciAPI CandidaYeastsAPI 20 C AUXY eastsAPI 20 AAnaerobesAPI CoryneCorynebacteriaAPI CampyCampylobacterAPI ListeriaListeriaAPI NHNeisseria, HaemophilusAPI 50 CHEE nterobacteriaceaeAP I 50 CHLL actic bacteriaAPI 50 CHBB acillusID 32 EGram-negative bacilliRapid id 32 EEnterobacteriaceaeID 32 GNGram-negative bacilliID 32 STAPHS taphylococciRapid id 32 STREPS treptococciID 32 CYeastsRapid id 32 AAnaerobes9 Abiotrophia defectiva Achromobacter denitrificans Alcaligenes denitrificansAchromobacter xylosoxidans Alcaligenes xylosoxidansAcinetobacter/Moraxella sppAcinetobacter/Pseudomonas sppAcinetobacter baumanniiAcinetobacter baumannii/calcoaceticusAcinetobacter calcoaceticusAcinetobacter haemolyticusAcinetobacter johnsoniiAcinetobacter juniiAcinetobacter junii/johnsoniiAcinetobacter lwoffiiAcinetobacter radioresistensAcinetobacter sppActinobacillus pleuropneumoniaeActinomyces

5 IsraeliiActinomyces meyeriActinomyces meyeri/odontolyticusActinomyces naeslundiiActinomyces neuii ssp anitratusActinomyces neuii ssp neuiiActinomyces odontolyticusActinomyces radingaeActinomyces turicensisActinomyces viscosusAeromonas caviaeAeromonas hydrophila/caviaeAeromonas hydrophilaAeromonas hydrophila/caviae/sobriaAeromonas salmonicida ssp achromogenesAeromonas salmonicida masoucida/achromogenesAeromonas salmonicida ssp masoucidaAeromonas salmonicida ssp salmonicidaAeromonas sobriaAerococcus urinaeAerococcus viridansAggregatibacter actinomycetemcomitansAggregatibacter aphrophilusAlcaligenes faecalisAlcaligenes sppAlloiococcus otitisAnaerobiospirillum succiniciproducensAnaerococcus prevotiiAneurinibacillus aneurinilyticusArcanobacterium haemolyticumArcobacter cryaerophilusArthrobacter sppAvibacterium paragallinarumBacillus amyloliquefaciensBacillus anthracisAPI 20 EAPI 10 SRapid 20 ETMAPI 20 NEAPI StaphAPI 20 StrepAPI CandidaAPI 20 C AUXAPI 20 AAPI CoryneAPI CampyAPI ListeriaAPI NHAPI 50 CHEAPI 50 CHLAPI 50 CHBID 32 ERapid id 32 EID 32 GNID 32 StaphRapid id 32 StrepID 32 CRapid id 32 A10 Species present in database Species identifiable with additional tests Changed taxon New species Bacillus badiusBacillus cereusBacillus circulansBacillus coagulansBacillus firmusBacillus lentusBacillus licheniformisBacillus megateriumBacillus mycoidesBacillus pumilusBacillus smithiiBacillus subtilisBacillus subtilis/amyloliquefaciensBacillus thuringiensisBacteroides

6 CaccaeBacteroides eggerthiiBacteroides fragilisBacteroides ovatusBacteroides ovatus/thetaiotaomicronBacteroides stercoris/eggerthiiBacteroides stercorisBacteroides thetaiotaomicronBacteroides uniformisBacteroides vulgatusBacillus non reactiveBergeyella zoohelcumBibersteinia trehalosiBifidobacterium adolescentisBifidobacterium bifidumBifidobacterium breveBifidobacterium dentiumBifidobacterium longumBifidobacterium sppBordetella/Alcaligenes/Moraxella sppBordetella aviumBordetella bronchisepticaBordetella sppBrevundimonas diminuta/Oligella urethralisBrevundimonas diminutaBrevundimonas vesicularisBrevibacillus agriBrevibacillus borstelensisBrevibacillus brevisBrevibacillus centrosporusBrevibacillus choshinensisBrevibacillus laterosporusBrevibacillus non reactiveBrevibacterium caseiBrevibacterium epidermidisBrevibacterium sppBrochothrix thermosphacta11 API 20 EAPI 10 SRapid 20 ETMAPI 20 NEAPI StaphAPI 20 StrepAPI CandidaAPI 20 C AUXAPI 20 AAPI CoryneAPI CampyAPI ListeriaAPI NHAPI 50 CHEAPI 50 CHLAPI 50 CHBID 32 ERapid id 32 EID 32 GNID 32 StaphRapid id 32 StrepID 32 CRapid id 32 ABrucella sppBudvicia aquaticaBurkholderia cepaciaBurkholderia gladioliBurkholderia pseudomalleiButtiauxella agrestisCandida albicansCandida boidiniiCandida ciferriiCandida colliculosaCandida dattilaCandida dubliniensisCandida famataCandida glabrataCandida globosaCandida guilliermondiiCandida hellenicaCandida holmiiCandida inconspicua/norvegensisCandida inconspicuaCandida

7 IntermediaCandida kefyrCandida kruseiCandida krusei/inconspicuaCandida lambicaCandida lipolyticaCandida lusitaniaeCandida magnoliaeCandida melibiosicaCandida membranifaciensCandida norvegensisCandida norvegicaCandida parapsilosisCandida pelliculosaCandida pulcherrimaCandida rugosaCandida sakeCandida silvicolaCandida sphericaCandida thermophilaCandida tropicalisCandida utilisCandida validaCandida zeylanoidesCampylobacter coliCampylobacter fetus ssp venerealisCampylobacter fetus ssp fetusCampylobacter hyointestinalisCampylobacter jejuni ssp doyleiCampylobacter jejuni ssp jejuniCampylobacter lari12 Species present in database Species identifiable with additional tests Changed taxon New speciesAPI 20 EAPI 10 SRapid 20 ETMAPI 20 NEAPI StaphAPI 20 StrepAPI CandidaAPI 20 C AUXAPI 20 AAPI CoryneAPI CampyAPI ListeriaAPI NHAPI 50 CHEAPI 50 CHLAPI 50 CHBID 32 ERapid id 32 EID 32 GNID 32 StaphRapid id 32 StrepID 32 CRapid id 32 ACampylobacter lari UPTCC ampylobacter mucosalisCampylobacter sputorum ssp bubulusCampylobacter sputorum bv FecalisCampylobacter sputorum bv SputorumCampylobacter upsaliensisCampylobacter ureolyticusCapnocytophaga gingivalisCapnocytophaga ochraceaCapnocytophaga sputigenaCapnocytophaga sppCarnobacterium divergensCarnobacterium maltaromaticumCedecea davisaeCedecea lapageiCedecea lapagei/neteriCedecea neteriCedecea sppCellulomonas spp/Microbacterium sppCellulomonas sppCellulosimicrobium cellulansChromobacterium violaceumChryseobacterium

8 IndologenesCitrobacter amalonaticus/farmeriCitrobacter amalonaticusCitrobacter braakiiCitrobacter farmeriCitrobacter freundiiCitrobacter freundii groupCitrobacter koseri/farmeriCitrobacter koseriCitrobacter koseri/amalonaticusCitrobacter sedlakiiCitrobacter youngaeClostridium acetobutylicumClostridium baratiiClostridium beijerinckii/butyricumClostridium beijerinckiiClostridium bifermentansClostridium botulinumClostridium botulinum/sporogenesClostridium butyricumClostridium cadaverisClostridium clostridioformeClostridium difficileClostridium fallaxClostridium glycolicumClostridium histolyticumClostridium innocuumClostridium limosumClostridium paraputrificum13 API 20 EAPI 10 SRapid 20 ETMAPI 20 NEAPI StaphAPI 20 StrepAPI CandidaAPI 20 C AUXAPI 20 AAPI CoryneAPI CampyAPI ListeriaAPI NHAPI 50 CHEAPI 50 CHLAPI 50 CHBID 32 ERapid id 32 EID 32 GNID 32 StaphRapid id 32 StrepID 32 CRapid id 32 AClostridium perfringensClostridium ramosumClostridium septicumClostridium sordelliiClostridium sporogenesClostridium subterminaleClostridium tertiumClostridium tetaniClostridium tyrobutyricumClostridium sppCollinsella aerofaciensComamonas testosteroni/Pseudomonas alcaligenesComamonas testosteroniComamonas sppCorynebacterium accolensCorynebacterium afermentans/coyleaeCorynebacterium afermentansCorynebacterium amycolatumCorynebacterium argentoratenseCorynebacterium aurisCorynebacterium auris/Turicella otitidisCorynebacterium bovisCorynebacterium coyleaeCorynebacterium

9 CystitidisCorynebacterium diphtheriae biotype belfantiCorynebacterium diphtheriae biotype gravisCorynebacterium diphtheriae biotype intermediusCorynebacterium diphtheriae biotype mitis/belfantiCorynebacterium diphtheriae mitisCorynebacterium glucuronolyticumCorynebacterium group F-1 Corynebacterium group GCorynebacterium jeikeiumCorynebacterium kutscheriCorynebacterium macginleyiCorynebacterium minutissimumCorynebacterium pilosumCorynebacterium propinquumCorynebacterium pseudodiphtheriticumCorynebacterium pseudotuberculosisCorynebacterium renaleCorynebacterium renale groupCorynebacterium seminale ( )Corynebacterium striatum/amycolatumCorynebacterium striatumCorynebacterium ulceransCorynebacterium urealyticumCronobacter dublinensis ssp dublinensisCronobacter dublinensis ssp lactaridiCronobacter dublinensis ssp lausannensisCronobacter dublinensis14 Species present in database Species identifiable with additional tests Changed