APPLICATION FOR FEDERAL ASSISTANCE 3. DATE RECEIVED …

1 APPLICATION FOR FEDERAL ASSISTANCE SF 424 (R&R) OMB Number: 4040-0001 Expiration Date: 06/30/2011 ! " # 1. * TYPE OF SUBMISSIONPre-applicationApplicationChan ged/Corrected APPLICATION :2. DATE SUBMITTED02/11/2010 Applicant Identifier3. DATE RECEIVED BY STATES tate APPLICATION Identifier4. a. FEDERAL Identifierb. Agency Routing Identifier5. APPLICANT INFORMATION* Organizational DUNS:* Legal NameColorado State UniversityDepartment:Division:* Street1:601 S. Howes StreetStreet2:* City:Fort CollinsCounty / Parish:* State:CO: ColoradoProvince:* Country:USA: UNITED STATES* ZIP / Postal Code:80523-2002 Person to be contacted on matters involving this applicationPrefix:* First Name:ChristineMiddle Name:* Last Name:GetzelmanSuffix:* Phone Number:Fax Number:Email:6. * EMPLOYER IDENTIFICATION (EIN) or (TIN): 7. * TYPE OF APPLICANT:H: Public/State Controlled Institution of Higher EducationOther (Specify):Small Business Organization TypeWomen Owned8.

2 * TYPE OF APPLICATION :NewResubmissionRenewalContin uationRevisionSocially and Economically DisadvantagedA. Increase AwardB. Decrease AwardC. Increase Duration mark appropriate box(es).If Revision,D. Decrease DurationE. Other (specify):* Is this APPLICATION being submitted to other agencies?YesNoWhat other Agencies?9. * NAME OF FEDERAL AGENCY:National Institutes of Health10. CATALOG OF FEDERAL DOMESTIC ASSISTANCE NUMBER:TITLE:11. * DESCRIPTIVE TITLE OF APPLICANT'S PROJECT:Mechanisms of Enteric Burkholderia psuedomallei infection12. PROPOSED PROJECT:* Start Date08/01/2010* Ending Date07/31/2012* 13. CONGRESSIONAL DISTRICT OF APPLICANTCO-00414. PROJECT DIRECTOR/PRINCIPAL INVESTIGATOR CONTACT INFORMATIONP refix:Dr.* First Name:StevenMiddle Name:WDow* Last Name:Suffix:Position/Title:Professor* Organization Name:Colorado State UniversityDepartment:Clinical SciencesDivision:CVMBS* Street1:1678 Campus DeliveryStreet2:* City:Fort CollinsCounty / Parish:* State:CO: ColoradoProvince:* Country:USA: UNITED STATES* ZIP / Postal Code:80523-1678* Phone Number:Fax Number:* Email: Always follow your funding opportunity's instructions for APPLICATION format.

3 Although this APPLICATION demonstrates good grantsmanship, time has passed since the grantee applied. The sample may not reflect the latest format or rules. NIAID posts new samples periodically: The text of the APPLICATION is copyrighted. You may use it only for nonprofit educational purposes provided the document remains unchanged and the PI, the grantee organization, and NIAID are credited. Note on Section 508 conformance and accessibility: We have reformatted these samples to improve accessibility for people with disabilities and users of assistive technology. If you have trouble accessing the content, please contact the NIAID Office of Knowledge and Educational Resources at PI: Dow, Steven W. Title: Mechanisms of Enteric Burkholderia psuedomallei infection RECEIVED : 02/11/2010 FOA: PA10-069 Council: 10/2010 Competition ID: ADOBE-FORMS-B FOA Title: NIH EXPLORATORY DEVELOPMENTAL RESEARCH GRANT PROGRAM (PARENT R21) 1 R21 AI091991-01 Dual: Accession Number: 3269078 IPF: 1725201 Organization: COLORADO STATE UNIVERSITY-FORT COLLINS Former Number: Department: Clinical Sciences IRG/SRG: ZRG1 IDM-A (80)S AIDS: N Expedited: N Subtotal Direct Costs (excludes consortium F&A) Year 1: Year 2: Animals: Y Humans: N Clinical Trial: N Current HS Code: 10 HESC: N New Investigator: N Early Stage Investigator: N Senior/Key Personnel: Organization: Role Category.

4 Steven Dow Colorado State University PD/PI Mercedes Gonzalez-Juarrero Colorado State University Other Professional-Co-Investigator Herbert Schweizer Colorado State University Other Professional-Consultant SF 424 (R&R) APPLICATION FOR FEDERAL ASSISTANCEPage 215. ESTIMATED PROJECT FUNDINGTHIS PREAPPLICATION/ APPLICATION WAS MADE a. Total FEDERAL Funds Requestedc. Total FEDERAL & Non- FEDERAL Fundsb. Total Non- FEDERAL Fundsd. Estimated Program Income398, , Sponsored ProgramsView AttachmentDelete AttachmentAdd AttachmentAdd AttachmentDelete AttachmentView Attachment ! " # 16. * IS APPLICATION SUBJECT TO REVIEW BY STATE EXECUTIVE ORDER 12372 PROCESS?a. YESAVAILABLE TO THE STATE EXECUTIVE ORDER 12372 PROCESS FOR REVIEW ON:DATE:b. NOPROGRAM IS NOT COVERED BY 12372; OR PROGRAM HAS NOT BEEN SELECTED BY STATE FOR REVIEW17. By signing this APPLICATION , I certify (1) to the statements contained in the list of certifications* and (2) that the statements herein are true, complete and accurate to the best of my knowledge.

5 I also provide the required assurances * and agree to comply with any resulting terms if I accept an award. I am aware that any false, fictitious. or fraudulent statements or claims may subject me to criminal, civil, or administrative penalities. ( Code, Title 18, Section 1001)* I agree* The list of certifications and assurances, or an Internet site where you may obtain this list, is contained in the announcement or agency specific SFLLL or other Explanatory Documentation19. Authorized RepresentativePrefix:* First Name:LindaMiddle Name:* Last Name:MonumSuffix:* Position/Title:Research Administrator* Organization:Colorado State UniversityDepartment:Division:* Street1:601 S. Howes StreetStreet2:* City:Fort CollinsCounty / Parish:* State:CO: ColoradoProvince:* Country:USA: UNITED STATES* ZIP / Postal Code:80526-1678* Phone Number:Fax Number:* Email:* Signature of Authorized RepresentativeLinda Monum* Date Signed02/11/201020.

6 Pre-application424 R&R and PHS-398 SpecificTable Of ContentsPage NumbersSF 424 R&R Face Page------------------------------------ ---------------------------------------- --------------1 Table of Contents-------------------------------- ---------------------------------------- ---------------------3 Performance Sites----------------------------------- ---------------------------------------- ------------------4 Research & Related Other Project Information----------------------------- -------------------------------------5 Project Summary/Abstract (Description)--------------------------- -------------6 Public Health Relevance Statement (Narrative attachment)----------------------------- -----------7 Facilities & Other Resources------------------------------- ---------8 Equipment------------------------------- ---------9 Research & Related Senior/Key Person---------------------------------- ---------------------------------------- 10 Biographical Sketches for each listed Senior/Key Person---------------------------------- ------12 phs 398 Specific Cover Page Supplement------------------------------ ---------------------------------------- --23 phs 398 Specific Modular Budget---------------------------------- ---------------------------------------- -----25 Personnel Justification--------------------------- -------------28 phs 398 Specific Research Plan------------------------------------ ---------------------------------------- ----29 Specific

7 Aims------------------------------------ ----30 Research Strategy-------------------------------- --------31 Vertebrate Animals--------------------------------- -------37 Select Agent Research-------------------------------- --------38 Bibliography & References Cited----------------------------------- -----39 Letters of Support--------------------------------- -------42 Resource Sharing Plan------------------------------------ ----46 phs 398 Checklist------------------------------- ---------------------------------------- ----------------------47 Principal Investigator/Program Director (Last, first, middle): Dow, Steven, W Table of Contents Page 3 Project/Performance Site Location(s)OMB Number: 4040-0010 Expiration Date: 08/31/2011 Project/Performance Site Primary LocationI am submitting an APPLICATION as an individual, and not on behalf of a company, state, local or tribal government, academia, or other type of organization.

8 Organization Name:Colorado State UniversityDUNS Number:* Street1:300 W. Drake RoadStreet2:* City: Fort CollinsCounty:* State:CO: ColoradoProvince:* Country:USA: UNITED STATES* ZIP / Postal Code:80523-1678* Project/ Performance Site Congressional District: CO-004 Project/Performance Site Location1 USA: UNITED STATESI am submitting an APPLICATION as an individual, and not on behalf of a company, state, local or tribal government, academia, or other type of organization. Organization Name:DUNS Number:* Street1:Street2:* City: County:* State:Province:* Country:* ZIP / Postal Code:* Project/ Performance Site Congressional District: Additional Location(s)View AttachmentDelete AttachmentAdd Attachment ! " # Principal Investigator/Program Director (Last, first, middle): Dow, Steven, W Performance Sites Page 4 1. * Are Human Subjects Involved?

9 IRB Approval Date:Human Subject Assurance Number:2. * Are Vertebrate Animals Used?IACUC Approval Date:Animal Welfare Assurance If yes, please If this project has an actual or potential impact on the environment, has an exemption been authorized or an environmental assessment (EA) or environmental impact statement (EIS) been performed? If yes, please explain:6. * Does this project involve activities outside of the United States or partnerships with international collaborators? Optional Explanation:7. * Project Summary/Abstract8. * Project Narrative11. Equipment12. Other AttachmentsRESEARCH & RELATED Other Project InformationIs the IACUC review Pending?If no, is the IRB review Pending? YES to Vertebrate Animals3. * Is proprietary/privileged information included in the APPLICATION ? * Does this project have an actual or potential impact on the environment? If yes, identify countries:1234569.

10 Bibliography & References Cited10. Facilities & Other YES to Human SubjectsYesNoYesNoIf yes, check appropriate exemption AttachmentDelete AttachmentView AttachmentDelete AttachmentAdd Attachment1235-Relevance AttachmentDelete AttachmentAdd Attachment1236-Literature AttachmentDelete AttachmentAdd AttachmentDelete AttachmentAdd MAJOR AttachmentsDelete AttachmentsAdd AttachmentsIs the Project Exempt from FEDERAL regulations?5. * Is the research performance site designated, or eligible to be designated, as a historic place? If yes, please explain: ! " # Principal Investigator/Program Director (Last, first, middle): Dow, Steven, W Other Information Page 5 Burkholderia pseudomallei (Bp) is a Gram-negative bacterial pathogen that can cause a variety of difficult-to-treat infections in humans ranging from acute sepsis to chronic abscesses.