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Bacterial genomic DNA isolation using CTAB
Oct 23, 2012 · Cell preparation and extraction techniques. (Modification of “CTAB method”, in Current Protocols in Molecular Biology) Cell growth: To minimize gDNA sampling bias (e.g., excess coverage of sequences around the origin of replication) please take precautions NOT to proceed with DNA isolation while most of the cell population is in the
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Genomic DNA QC Using Standard Gel Electrophoresis (For ...
jgi.doe.gov1.1 Cast a ~100ml 1% agarose gel with 1X TAE and ethidium bromide (.15ug/ml) or SYBR® Safe DNA gel stain (10,000X concentrate in DMSO). Use a narrow well comb. 1.2 Transfer 1µl of your genomic DNA sample(s) (concentration between 50ng to 500ng) into clean labeled tube(s) and bring the total volume up to 4µl with 1X TE Buffer, pH 8.0. a.