Transcription of Basic Practical Microbiology
1 Microbiology SocietyBasic Practical MicrobiologyA MANUALA bout this resourceCopyrightMicrobiology is a popular option for Practical work in schools. This manual, which explains the Basic techniques necessary to carry out Microbiology experiments safely and effectively, is intended as a guide for teachers and Microbiology Society, in association with the Microbiology in Schools Advisory Committee (MiSAC) has also produced a book of 21 Practical investigations which complements the manual Practical Microbiology for Secondary Schools is suitable for use with Key Stages 3, 4 and post-16 and the equivalent Scottish qualifications.
2 This resource can be downloaded from the Microbiology Society s dedicated education website: Microbiology Society offers schools membership and runs an advice service on Microbiology teaching. For further details, email telephone +44 (0)20 7685 2682 or write to the address at the bottom of the page. Other useful sources of advice: x CLEAPSS The Gardiner Building, Brunel Science Park, Uxbridge UB8 3 PQx Microbiology in Schools Advisory Committee (MiSAC) c/o NCBE (see address below)x National Centre for Biotechnology Education (NCBE) Science and Technology Centre, The University of Reading, 2 Earley Gate, Whiteknights, Reading RG6 6 AUx Scottish Schools Equipment Research Centre (SSERC)
3 2 Pitreavie Court, South Pitreavie Business Park, Dunfermline KY11 8 UBPublished by the Microbiology Society, Charles Darwin House, 12 Roger Street, London WC1N 2JU, UK 2016 Microbiology SocietyAbout this resourceMicrobiology is a popular option for Practical work in schools. This manual, which explains the basictechniques necessary to carry out Microbiology experiments safely and effectively, is intended as aguide for teachers and technicians. It forms the notes for the one-day Basic Practical microbiologytraining course which is run and accredited by the Society for General Microbiology (SGM), but canbe used as a standalone document.
4 Further information about the training courses is available on theSGM s education website (see below), which also includes a wealth of other material relating to bothpractical and theoretical Microbiology SGM, in association with the Microbiology in Schools Advisory Committee (MISAC) has also produced a book of 21 Practical investigations which complements the manual: PracticalMicrobiology for Secondary Schoolsis suitable for use with Key Stages 3, 4 and post-16 and theequivalent Scottish qualifications. Ordering details are available on the website noted SGM offers schools membership and runs an advice service on Microbiology teaching.
5 For further details, email telephone +44 (0)118 988 1835, fax +44 (0)118 9885656 or write to the address at the bottom of the page. Much information can be found on the website: useful sources of advice: CLEAPSS University, Uxbridge UB8 3PH Microbiology in Schools Advisory Committee (MISAC) House, Basingstoke Road, Spencers Wood, Reading RG7 1AG National Centre for Biotechnology Education (NCBE) and Technology Centre, The University of Reading, Earley Gate, PO Box 247, Reading RG6 6BZ Scottish Schools Equipment Research Centre (SSERC) Pitreavie Court, South Pitreavie Business Park, Dunfermline KY11 8 UBCredits & AcknowledgementsThe SGM gratefully acknowledges the support from the following sources.
6 Kath Crawford (SAPS Scotland) John Richardson (SSERC) Members of MISAC John Schollar (NCBE) John Tranter (CLEAPSS)Editors: Dariel Burdass, John Grainger & Janet HurstDesign and Production Editor: Ian AthertonPhotographs: Dariel Burdass & Faye JonesFront cover illustration: TEK Image / Science Photo LibraryISBN 0 95368 383 4 CopyrightBasic Practical Microbiology A Manualis copyright. The Society for General Microbiology assertsits moral right to be identified as copyright holder under Section 77 of the Designs, Patents andCopyright Act (UK) use:Electronic or paper copies of the resource or individual pages from it may be made for classroom and bona fide educational uses, provided that the copies are distributedfree of charge or at the cost of reproduction and that the SGM is credited and identified as copyright by the Society for General Microbiology , Marlborough House, Basingstoke Road,Spencers Wood, Reading RG7 1AG, UK 2006 Society for General MicrobiologyBasic Pract Cover 2009 16/1/09 12.
7 01 Page 4 Credits & Acknowledgements x x x x x Wladimir Bulgar/Science Photo LibraryEducational use: Electronic or paper copies of the resource or individual pages from it may be made for classroom and bona fide educational uses, provided that the copies are distributed free of charge or at the cost of reproduction and that the Microbiology Society is credited and identified as copyright Practical Microbiology A Manual is copyright. The Microbiology Society asserts its moral right to be identified as copyright holder under Section 77 of the Designs, Patents and Copyright Act (UK) 1988.
8 The Microbiology Society gratefully acknowledges the support from the following sources: MiSACC ontentsAbout this resourceInside front coverPart 1: The BasicsAn introduction to Microbiology , aseptic technique and safetyPreparationSafety guidelines1 Risk assessment2 Good microbiological laboratory practice (GMLP)3 Spillage management3 Aerosols3 ResourcesEquipment4 Apparatus5 Materials5 Media, sterilisation and disinfectionPreparation of culture media6 Pouring a plate6 Storage of media6 Sterilisation vs disinfection6 Sterilisation using the autoclave/pressure cooker 7 Sterilisation of equipment and materials 7 Choice.
9 Preparation and use of disinfectants7 Inoculation and other aseptic proceduresEssential points8 Using a wire loop8 Using a pipette9 Flaming the neck of bottles and test tubes10 Working with bacteria and yeastStreak plate11 Pour plate12 Using a spreader13 Spread plate14 Working with moulds15 Incubation16In conclusion: clearing up17 Basic Pract Book 2006 2/11/06 11:17 am Page 1 Essential methods for maintaining, preparing and using culturesObtaining suitable cultures18 Pure cultures18 Maintaining stock cultures18 Checking cultures for contamination18 Preventing contamination of cultures and the environment19 Aseptic transfer of cultures and sterile solutions19 Preparing cultures for class use19 Part 2: Microbiology in ActionPractical sensitivity to antimicrobial the microscope22 Stained preparations23 Making a smear23A simple stain24A differential stain.
10 Gram s staining of cultures and of the autoclave/pressure serial dilutions40 Basic Pract Book 2006 2/11/06 11:17 am Page 2 Basic Practical Microbiology A Manual 2006 SGM 1 Part 1: The BasicsAn introduction to Microbiology , aseptictechnique and safetyAs well as causing a familiar range of diseases in animals and plants and problems in food spoilage and deteriorationof other materials, microbes are also our invisible allies . Indeed, life on Earth would not be sustainable without the benefits that many of them provide.
