BD BBL Phytone Peptone BD Difco Select ... - BD …

1 BD BBL Phytone Peptone BD Difco Select Phytone UF BD Difco Select soytone BD Bacto SoytoneGrowth Curve1% BD BBL Phytone Peptone in M9 Minimal Salts + Glucose, Bioscreen in HoursAverage OD at 600 nmMolecular WeightBD BBL Phytone Peptone010203040506070 Area Percentage<250250-500500-2 k2k-5k5k-10k>10kMolecular Weight Range (daltons)Product DescriptionAll of the Difco and BBL brand soy peptones are enzymatic digests of soybean meal/flour. They are recommended for use in media for the cultivation of a wide variety of organisms, including fungi.

2 The soybean protein in these peptones contains naturally occurring high concentrations of vitamins and ApplicationsBDAB offers a diverse choice of soy peptones. The individual characteristics of each Peptone are the result of processing methods engineered to consistently deliver these characteristics from batch to batch. The nutritional requirements of microorganisms and cell lines vary according to each individual strain. While some organisms or cell lines may prefer short chain or free amino acids, others benefit from longer chain amino acids.

3 While the typical analysis profiles for each Peptone in this manual can help direct the users to the correct Peptone match, it is recommended that users supplement the typical analytical information with evaluations in their own individual growth BBL Phytone Peptone (animal-free) retains the high vitamin and high carbohydrate content of the soy plant tissue. It is an excellent plant Peptone for the cultivation of fungi and fastidious types of bacteria, such as members of the Clostridium and Neisseria genera.(1) It has been used in mammalian cell culture applications due to its high carbohydrate content.

4 BD Difco Select Phytone UF is an animal-free ultrafiltered Peptone developed specifically for the mammalian cell culture market. Its nitrogen content, combined with the naturally occurring vitamins, has demonstrated remarkable growth support with monoclonal antibodies and protein expression. It has an endotoxin level of less than or equal to 500 offers three other soy Peptone products suitable for a variety of bacterial Difco Select soytone (animal-free) demonstrates excellent growth support for Escherichia coli.

5 BD Difco Select soytone is also used in Select APS Super Broth. Subtle differences in the digestion process give BD Difco Select soytone improved performance in cell Biosciences Advanced PEPTONES AND YEAST EXTRACTS Growth Curve1% BD Difco Select Phytone UF in M9 Minimal Salts + Glucose, Bioscreen in HoursAverage OD at 600 nmMolecular WeightBD Difco Select Phytone UF010203040506070 Area Percentage<250250-500500-2 k2k-5k5k-10k>10kMolecular Weight Range (daltons)TitrationBD Difco Phytone UF Titration Hybridoma Proliferation and Production Data 0200040006000800010000013579111315170200 04000600080001000012000 Day 0 RFUDay 3 RFUDay 4 RFUDay 5 RFUDay 6 RFUDay 7 RFUDay 7 ng/mLRFUA ntibody Yield (ng/mL) Peptone Concentration (g/L)BD Bacto soytone was found to be effective in the recovery of stressed Escherichia coli.

6 (2) It was found that BD Bacto soytone , with the addition of seven vitamins, replaced yeast extract as an economical alternative for the production of lactic acid by Lactobacillus rhamnosus.(3) BD Bacto soytone utilizes an animal based enzyme in the digestion of the soy CharacteristicsBD BBL Phytone Peptone is a fine homogeneous powder free of extraneous material. BD Difco Select Phytone UF is a fine homogeneous powder free of extraneous Difco Select soytone is a fine homogeneous powder free of extraneous Bacto soytone is a light to medium tan, free-flowing, homogeneous Bionutrients Technical ManualFourth EditionANIMAL-FREE PEPTONES AND YEAST EXTRACTSG rowth Curve1% BD Difco Select soytone in M9 Minimal Salts + Glucose.

7 Bioscreen C OD at 600 nmTime in HoursMolecular WeightBD Difco Select Soytone010203040506070 Area Percentage<250250-500500-2 k2k-5k5k-10k>10kMolecular Weight Range (daltons)TitrationBD Difco Select soytone TitrationCHO Proliferation and Production Data020004000600080001000012000013579111 31517020004000600080001000012000 Day 0 RFUDay 3 RFUDay 4 RFUDay 5 RFUDay 6 RFUDay 7 RFUDay 7 ng/mLRFUA ntibody Yield (ng/mL) Peptone Concentration (g/L)AvailabilityProduct Description Cat. BBL Phytone Peptone211906454gBD BBL Phytone Peptone2981475lb ( )

8 BD BBL Phytone Peptone29245010kgBD Difco Select Phytone UF210931500gBD Difco Select Phytone UF21093610kgBD Difco Select Soytone212488500gBD Difco Select Soytone21248910kgBD Bacto soytone *243620500gBD Bacto soytone *24361010kg*Utilizes an animal-based enzyme in the digestion of the soy flour21BD Biosciences Advanced PEPTONES AND YEAST EXTRACTS Growth Curve1% BD Bacto soytone in M9 Minimal Salts + Glucose, Bioscreen C in HoursAverage OD at 600 nmMolecular WeightBD Bacto Soytone010203040506070 Area Percentage<250250-500500-2 k2k-5k5k-10k>10kMolecular Weight Range (daltons)References1.

9 Power (ed). 1988. Manual of BBL products and laboratory procedures, 6th ed. Becton Dickinson Microbiology Systems, Cockeysville, Chou and Cheng. 2000. Recovery of low-temperature stressed E. coli O157:H7 and its susceptibility to crystal violet, bile salt, sodium chloride and ethanol. Int. J. Food Microbiol. 61 Kwon, Lee, Lee, Chang, Keun and Chang. 2000. Production of lactic acid by Lactobacillus rhamnosus with vitamin-supplemented soybean hydrolysate. Enzyme Microb. Technol. 26 Bionutrients Technical ManualFourth EditionANIMAL-FREE PEPTONES AND YEAST EXTRACTS