BD LSRFortessa X-20 Cell Analyzer User’s Guide

1 23-11947-0110/2020 For Research Use OnlyBecton, Dickinson and Company BD Biosciences2350 Qume Drive San Jose, CA 95131 USA Biosciences European Customer SupportTel + LSRF ortessa X-20 Cell Analyzer User s GuideCopyrights 2020, Becton, Dickinson and Company. All rights reserved. No part of this publication may be reproduced, transmitted, transcribed, stored in retrieval systems, or translated into any language or computer language, in any form or by any means: electronic, mechanical, magnetic, optical, chemical, manual, or otherwise, without prior written permission from BD information in this Guide is subject to change without notice.

2 BD Biosciences reserves the right to change its products and services at any time to incorporate the latest technological developments. Although this Guide has been prepared with every precaution to ensure accuracy, BD Biosciences assumes no liability for any errors or omissions, nor for any damages resulting from the application or use of this information. BD Biosciences welcomes customer input on corrections and suggestions for , the BD Logo, FACS, FACSDiva, FACSFlow, and FACSC lean are trademarks of Becton, Dickinson and Company or its affiliates. All other trademarks are the property of their respective owners. 2020 BD. All rights Fluor and Pacific Blue are trademarks of Life Technologies is a trademark of GE Healthcare.

3 Cy dyes are subject to proprietary rights of GE Healthcare and Carnegie Mellon University and are made and sold under license from GE Healthcare only for research and in vitro diagnostic use. Any other use requires a commercial sublicense from GE Healthcare, 800 Centennial Avenue, Piscataway, NJ 08855-1327, : US 5,714,386 Regulatory informationFor Research Use Only. Not for use in diagnostic or therapeutic 1 Laser ProductFCC informationWARNING: Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user s authority to operate the : This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules.

4 These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause harmful interference to radio communications. Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his or her own expense. Shielded cables must be used with this unit to ensure compliance with the Class A FCC limits. This Class A digital apparatus meets all requirements of the Canadian Interference-Causing Equipment Regulations.

5 Cet appareil num rique de la classe A respecte toutes les exigences du R glement sur le mat riel brouilleur du informationNOTICE: This laboratory equipment has been tested and found to comply with the EMC and the Low Voltage Directives. This includes FCC, Part 15 compliance for a Class A Digital : Any unauthorized modifications to this laboratory equipment may affect the Regulatory Compliance items stated made23-11947-004/2013 Initial release23-11947-0110/2020 Removed references of FACSR inse and added BD Detergent Solution Concentrate in place of 1: About this guide9 What this Guide covers .. 10 Conventions .. 11 About the BD LSRF ortessa X-20 documentation.

6 11 Instrument technical support .. 13 Chapter 2: Introduction15 Instrument overview .. 16 Components .. 17 Fluidics .. 19 Sheath and waste containers .. 23 Optics .. 23 Workstation .. 27 Chapter 3: Cytometer setup29 Starting the cytometer and computer .. 30 Preparing the sheath container .. 31 Removing air bubbles .. 33 Preparing the waste container .. 36 Priming the fluidics .. 39 About the optical filters and mirrors .. 40 Changing optical filters and mirrors .. 42 Custom configurations and baselines .. 43BD LSRF ortessa X-20 Cell Analyzer User s Guide6 Chapter 4: Maintenance45 Maintenance overview .. 46 Cleaning the fluidics.

7 47 Shutting down the cytometer .. 49 Flushing the system .. 50 Maintaining the waste management system .. 52 Replacing the waste air filter .. 55 Changing the sheath filter .. 56 Changing the Bal seal .. 58 Changing the sample tube O-ring .. 60 Chapter 5: Optimizing cytometer settings63 Cytometer settings workflow .. 64 Verifying the configuration and user preferences .. 66 Running a performance check .. 68 Setting up an experiment .. 72 Creating application settings .. 76 Recording compensation controls .. 79 Calculating compensation .. 82 Chapter 6: Recording and analyzing data85 Data recording and analysis workflow .. 86 Preparing the workspace.

8 87 Recording data .. 88 Analyzing data .. 91 Reusing an analysis .. 97 Chapter 7: Technical overview99 About fluidics .. 100 About optics .. 101 About electronics .. 112 Contents7 Chapter 8: Troubleshooting117 Cytometer troubleshooting .. 118 Electronics troubleshooting .. 127 Chapter 9: Detector array configurations129 Fluorescence spectra .. 130 About configuration maps .. 132 About the base configuration .. 133 Base configuration polygon maps .. 137 Special order configurations .. 150 Chapter 10: Manual settings165 About laser delay .. 166 Optimizing laser delay .. 167 Adjusting area scaling .. 169 Chapter 11: Supplies and consumables175 Ordering information.

9 176 Beads .. 176 Reagents .. 177 Equipment .. 178 Index1791 About this guideThis chapter covers the following topics: What this Guide covers (page 10) Conventions (page 11) About the BD LSRF ortessa X-20 documentation (page 11) Instrument technical support (page 13)BD LSRF ortessa X-20 Cell Analyzer User s Guide10 What this Guide coversThis Guide describes the procedures necessary to operate and maintain your special order BD LSRF ortessa X-20 cell Analyzer . Because many cytometer functions are controlled by BD FACSDiva software, this Guide also contains information about software features required for basic cytometer setup and operation.

10 This Guide assumes you have a working knowledge of basic Microsoft Windows operation. If you are not familiar with the Windows operating system, see the documentation provided with your 1: About this guide11 ConventionsIntroductionThe following table lists the safety symbols used in this Guide to alert you to potential symbolsAbout the BD LSRF ortessa X-20 documentationIntroductionThis topic describes the documentation available with the special order BD LSRF ortessa X-20 cell formats This Guide is provided in PDF format to provide an eco-friendly option. All content is also included in the BD FACSDiva software Help. SymbolMeaningCaution alertIdentifies a hazard or unsafe practice that could result in data loss, material damage, minor injury, severe injury, or deathBiological hazardElectrical hazardLaser hazardBD LSRF ortessa X-20 Cell Analyzer User s Guide12 Help systemThe help system installed with BD FACSDiva software includes all content from this Guide and the documents listed below.