Beef Extract Powder • Bacto Beef Extract, …

1 74 Section IIIBR eferences1. Baird-Parker. 1962. J. Appl. Bacteriol. 25 Baird-Parker. 1962. J. Appl. Bacteriol. 25 Baird-Parker. 1963. J. Gen. Microbiol. 30 Baird-Parker. 1965. J. Gen. Microbiol. 38 Tardio and Baer. 1971. J. Assoc. Off. Anal. Chem. 54 Baer. 1971. J. Assoc. Off. Anal. Chem. 54 Horwitz (ed.). 2000. Official methods of analysis of AOAC International, 17th ed., vol. 1. AOACI nternational, Gaithersburg, United States Phamacopeial Convention, Inc. 2001. The United States pharmacopeia 25/The na-tional formulary 20 2002.

2 United States Pharmacopeial Convention, Inc., Rockville, Baird-Parker Agar BaseAOAC BAM CCAM COMPF EP ISO SMD SMWW USDA USPCat. 500 g276810 Dehydrated 2 kgDifco EY Tellurite EnrichmentAOAC BAM CCAM COMPF EP ISO SMD SMWW USDA USPCat. 6 x 100 mL*BBL Baird-Parker AgarAOAC BAM CCAM COMPF EP ISO SMD SMWW USDA USPU nited States and CanadaCat. Plates (complete) Pkg. of 20*297725 Prepared Plates (complete) Ctn. of 100*EuropeCat. Plates (complete) Pkg. of 20*MexicoCat. Plates (complete) Pkg.

3 Of 10**Store at 2-8 Extract Powder Bacto beef Extract ,Desiccated beef ExtractIntended UseBeef Extract Powder , Bacto beef Extract , Desiccated and BeefExtract (paste) are used in preparing microbiological and ExplanationBeef Extract is intended to replace aqueous infusion of meat inmicrobiological culture media. beef Extract is frequently usedat a concentration of to in culture media, althoughconcentrations may vary depending on the nutritional require-ments for the medium formulation. beef Extract may be reliedupon for biochemical studies, particularly fermentation reac-tions, because of its independence from fermentable substancesthat would interfere with the accuracy of such Extract was used in media for early studies of nonsporu-lating anaerobes of the intestinal tract and as a stock broth inthe study of nutritional needs of streptococci.

4 Prokofeva et beef Extract for growing thermoacidophilic organismsnewly isolated from hot springs in Kamchatka, Russia. Kataokaand Tokiwa2 used beef Extract as a nitrogen source in studiesof mannose production by Clostridium tertium strains isolatedfrom soil and methanogenic sludge. In addition, beef Extractis a nutritive ingredient in many classical culture media,including Antibiotic Assay media described in The United StatesPharmacopeia,3 and several media recommended for standardmethods Extract Powder is a meat Extract dried to a beef Extract , Desiccated is the dried form of BeefExtract (paste) and was developed to provide a product forease of use in Extract is a meat Extract in paste products are to be used in a one for one substitution.

5 However, variations tend to be formulation-specific and requireactual performance of the ProcedureBeef Extract Powder , Bacto beef Extract , Desiccated and BeefExtract (paste) are derived from infusion of beef and providean undefined source of nutrients. These beef Extract productsare not exposed to the harsh treatment used for proteinhydrolysis, so they can provide some of the nutrients lostduring peptone beef Extract Powder , Bacto BeefExtract, Desiccated and beef Extract (paste) are mixtures ofpeptides and amino acids, nucleotide fractions, organic acids,minerals and some vitamins.

6 The function of these BeefExtract products can be described as complementing thenutritive properties of peptone by contributing minerals,phosphates, energy sources and those essential factors missingfrom AnalysisRefer to Product Tables in the Reference Guide section of for Preparation fromDehydrated ProductRefer to the final concentration of beef Extract Powder , beef Extract , Desiccated or beef Extract in the formula of themedium being prepared. Add appropriate product as appropriate references for specific procedures usingBeef Extract Powder , Bacto beef Extract , Desiccated or ResultsRefer to appropriate references and procedures for Agar, Quality ControlNOTE: Differences in the Identity Specifications and Cultural Response testing for media offered as both Difco and BBL brands may reflect differencesin the development and testing of media for industrial and clinical applications, per the referenced SpecificationsBBL beef Extract PowderDehydrated Appearance.

7 Fine, homogeneous, light to lightmedium, cream to solution, soluble in purifiedwater. Solution is light to light medium,yellow to tan, clear to slightly of at 25 C:pH ResponseBBL beef Extract PowderPrepare a sterile solution of peptone agar using g of beef ExtractPowder, g of sodium chloride and g of agar in 250 mL ofpurified water. Adjust final pH to Inoculate and incubate platesat 35 2 C for 2 days under appropriate atmospheric INOCULUM CFURECOVERYE scherichia coli25922102-103 GoodStaphylococcus aureus25923102-103 GoodStreptococcus pyogenes49117102-103 GoodReferences1.

8 Prokofeva, Miroshnichenko, Kostrikina, Chernyh, Kuznetsov, Tourova and Int. J. Syst. Evol. : Pt 6 Kataoka and Tokiwa. 1998. J. Appl. Microbiol. 84 United States Pharmacopeial Convention, Inc. 2001. The United States pharmacopeia 25/Thenational formulary 20 2002. United States Pharmacopeial Convention, Inc., Rockville, Clesceri, Greenberg and Eaton (ed.). 1998. Standard methods for the examination of water andwastewater, 20th ed. American Public Health Association, Washington, Food and Drug Administration.

9 1995. Bacteriological analytical manual, 8th ed. AOACI nternational, Gaithersburg, Downes and Ito (ed.). 2001. Compendium of methods for the microbiological examination offoods, 4th ed. American Public Health Association, Washington, Horwitz (ed.). 2000. Official methods of analysis of AOAC International , 17th ed. AOAC Inter-national, Gaithersburg, Department of Agriculture. 1998. Microbiology laboratory guidebook, 3rd ed. Food Safetyand Inspection Service, USDA, Washington, Cote. 1999. In Flickinger and Drew (ed.)

10 , Encyclopedia of bioprocess technology: fermentation,biocatalysis, and bioseparation. John Wiley & Sons, Inc., New York, Bridson and Brecker. 1970. In Norris and Ribbons (ed.), Methods in microbiology, vol. Press, New York. beef Extract PowderAOAC BAM COMPF SMWW USDA USPCat. 500 gBacto beef Extract , DesiccatedAOAC BAM COMPF SMWW USDA USPCat. 500 gDifco beef ExtractAOAC BAM COMPF SMWW USDA USPCat. 500 gBeef Heart for InfusionIdentity SpecificationsBacto beef Extract , DesiccatedDehydrated Appearance:Medium to dark brown solution, soluble in purifiedwater upon warming.