BIO 101 Worksheet Metabolism and Cellular Respiration

1 BIO 101 worksheet metabolism and cellular respiration Exam Unit 2. FIRST study notes and read textbook, review the list of objectives for unit 2 (Chapters 6, 7, 8, 9). Next, review homework assignments and worksheets Organelle review Worksheet cell biology Worksheet membrane transport Worksheet enzymes Worksheet Metabolism and Cellular Respiration Glycolysis True or false. If false, indicate why 1. _____Glycolysis takes place in the cristae of mitochondria 2. _____ An end product of glycolysis is pyruvate 3. _____ A 6 carbon sugar is oxidized in glycolysis 4. _____ CO2 is a waste product of glycolysis 5. _____ sugar + NAD+ pyruvate + NADH + 2 ATP represents glycolysis 6. _____ Glycolysis leads to fermentation in some bacteria and yeast 7. _____ Glycolysis involves an energy pay-off and then an energy investment phase 8.

2 _____ A net of 4 ATP are produced in glycolysis 9. _____ Pyruvate contains 3 carbons 10. _____ Glycolysis involves 10 steps tightly controlled by enzymes 11. _____ Glycolysis is an exergonic process that releases energy 12. _____ Glucose is modified to form acetyl CoA for the citric acid cycle 13. _____ Glycolysis does not use oxygen, it is anaerobic 14. _____ Glycolysis is the first step in Cellular Respiration 15. ___ It is likely that glycolysis was the method of ATP production in first organisms on Earth. 16. _____ Organisms that engage in glycolysis cannot engage in fermentation The Citric Acid Cycle True or false. If false, indicate why 1. _____ The purpose of the citric acid cycle is to remove excess oxygen from cells 2. _____ The initial molecule in the citric acid cycle is acetyl-CoA.

3 3. _____ The citric acid cycle occurs in the inner membrane of the mitochondria 4. _____ 1 glucose molecule leads to 2 turns of the citric acid cycle and produce 2 ATP. 5. _____ The citric acid cycle is a loosely controlled series of enzymatic steps 6. _____ All living organisms engage in the citric acid cycle 7. _____ Carbon dioxide is produced in the citric acid cycle 8. _____ Electron carriers oxygen and water are produced in the citric acid cycle 9. _____ The citric acid cycle is the second step in Cellular Respiration 10. _____ The citric acid cycle is also called the Crab cycle BIO 101 worksheet metabolism and cellular respiration Oxidative phosphorylation True or false. If false, indicate why. 1. _____ Oxidative phosphorylation involves the electron transport chain 2. _____ Oxidative phosphorylation occurs in the cytoplasm 3.

4 _____ Oxidative phosphorylation is an aerobic process 4. _____ Ca+2 ions are pumped into the mitochondrial matrix during electron transport 5. _____ Electrons are donated by NADH and FADH2. 6. _____ Energy is harvested in a step wise fashion 7. _____ Water is produced in oxidative phosphorylation 8. _____ ATP is broken down to 34 ADP + 34 P in oxidative phosphorylation 9. _____ATP is synthesized by a ATP synthase 10. _____ ATP synthase requires H+ ions to operate 11. _____ The total amount of ATP produced by all Cellular Respiration activities is ~ 24. 12. _____ Cellular Respiration can be represented by: C6H12O6 + CO2 - O2 + H2O + energy 13. _____ Oxidative phosphorylation cannot occur in prokaryotes 14. _____ Mitochondria are located in the plasma membrane of eukaryotic cells 15. _____ One cell may have thousands of mitochondria 16.

5 _____ lipids, carbohydrates, and proteins can serve as fuel for Cellular Respiration Fermentation True or false. If false, indicate why 1. _____ Fermentation is linked to the citric acid cycle 2. _____ Fermentation may produce ethanol 3. _____ Yeast and bacteria engage in fermentation 4. _____ Human cells can engage in fermentation 5. _____ Obligate anaerobes engage in fermentation 6. _____ Fermentation begins with pyruvate 7. _____ Fermentation does not produce CO2. 8. _____ Fermentation occurs in the cytoplasm 9. _____ Fermentation cannot produce ATP. 10. _____ Fermentation is linked to glycolysis Practice questions Metabolism and Cellular Respiration 1. Choose the pair of terms that completes this sentence: Catabolism is to anabolism as ___ is to __. a. Exergonic: spontaneous b. Exergonic: endergonic c.

6 Heat: entropy d. First law of thermodynamics: second law 2. Most cells cannot harness heat to perform work because a. Cells do not have much heat, they are relatively cool 10. b. Heat can never be used to do work c. Heat must remain constant during work d. Temperature is usually uniform throughout a cell BIO 101 worksheet metabolism and cellular respiration 3. Which of the following metabolic processes can occur without a net influx of energy? a. ADP + ATP + H2O. b. C6H12O6 + 6 O2 6 CO2 + 6 H2O. c. 6 CO2 + 6 H2O C6H12O6 + 6 O2. d. amino acids protein 4. If an enzyme in solution is saturated with substrate, the most effective way to obtain a faster yield of products is to a. add more enzyme. b. heat the solution to 90 C. c. add more substrate. d. add a noncompetitive inhibitor. 5. Some bacteria are metabolically active in hot springs because a.

7 They are able to maintain a lower internal temperature. b. high temperatures make catalysis unnecessary. c. their enzymes have high optimal temperatures d. their enzymes are insensitive to temperature 6. Using a series of arrows, draw the branched metabolic reaction pathway described by the following statements, and then answer the question at the end. Use red arrows and minus signs to indicate inhibition. L can form either M or N. M can form O. O can form either P or R. P can form Q. R can form S. O inhibits the reaction of L to form M. Q inhibits the reaction of O to form P. S inhibits the reaction of O to form R. Which reaction(s) would prevail if both Q and S were present in the cell in high concentrations? a. L M. b. M O. c. L N. d. O P. e. R S. 7. Glycolysis leads to production of ____ and 2 molecules of ATP.

8 In the absence of oxygen, fermentation leads to production of _____. Glycolysis plus the citric acid cycle can convert the carbons of glucose to _____, storing the energy as ATP, _____ and ____. A. lactic acid, pyruvate, CO2, NADH, FADH2. B. pyruvate, lactic acid, CO2, NADH, FADH2. C. CO2, NADH, FADH2, lactic acid, pyruvate D. O2, lactic acid, pyruvate, FADH2. E. glucose, lactic acid, CO2, NADH, FADH2. BIO 101 worksheet metabolism and cellular respiration 8. At the end of glycolysis, each molecule of glucose has yielded 2 molecules of _____, 2 molecules of _____, and a net of 2 molecules of _____. A. FAD; NAD+; ADP. B. CO2; NAD+; ADP. C. lactic acid; ethanol; CO2. D. pyruvate, NADH, ATP. E. H2O; CO2; ATP. 9. The electron transport chain is located predominantly in the: A. Outer membrane of the mitochondria B.

9 Intermembrane space of the mitochondria C. Inner membrane of the mitochondria D. Matrix of the mitochondria E. Cytoplasm of the cell 10. The terminal electron acceptor during mitochondrial Respiration : A. H2 0. B. NADH. D. ATP. E. O2. 11. ATP synthase can produce ATP using as a direct energy source: a. energy from the conversion of glucose to pyruvate b. energy from the oxidation of pyruvate producing CO2 and H20. c. energy from a proton (H+) gradient established in mitochondria d. energy derived from the breakdown of NADH and FADH2. Examine the following reaction, and then answer the questions below: catecholase catechol + oxygen (O2)---------------> polyphenol 12. The enzyme in this reaction a. catechol b. oxygen c. catecholase d. polyphenol 13.. The product in this reaction a. catechol b.

10 Oxygen c. catecholase d. polyphenol BIO 101 worksheet metabolism and cellular respiration 14. The substrate in this reaction a. catechol b. catecholase c. polyphenol d. ATP. 15. An enzyme is primarily made of: a. carbohydrate b. protein c. nucleic acid d. lipid 16. Competitive inhibitors stop an enzyme from working by . a. changing the shape of the enzyme b. merging with the substrate instead c. blocking the active site of the enzyme d. combining with the product of the reaction 17. When a molecule binds to an area of an enzyme that is not the active site, and changes the shape of the enzyme so that it no longer can work, this is a. denaturation b. competitive inhibition c. noncompetitive inhibition d. substrate delocation 18. In an enzymatic reaction, the amount of _____ determines the amount of product produced.