課題② 画像処理方法の演習 - bio.chuo-u.ac.jp

1 A-16 USB web ~kam/ Image J Image J USB Image J USB Image J OS Image J IT USB A-17 NIH Image Windows Scion Image Image J NIH 32-bit Image J 2-1 Web Pleurosigma part SinePattern Pleuropsigma A-18 i 1.

2 File Save as Text Jpg JPEG 8 256 0 255 File Import Text 2. Excel 16 P 3. (iii)-5 Straight line selection 4.

3 Image Adjust Brightness/Contrast Brightness Contrast 256 8-bit 5. Image Rotate Edit Invert Image Lookup Tables 6. Process Enhance Contrast 7.

4 Image Duplicate Process Filters Gaussian Blur (radius 5-10 A-19 ) Radius 8. Process Image Calculator Operation Subtract (4) 9.

5 (ii) Image J web 1. Process FFT FFT FFT of FFT FFT Fast Fourier Transform FFT FFT FFT 2.

6 Image J Elliptical or brush selections FFT Edit Cut Ctrl+X Edit Invert (Cnrl+Shift+I) A-20 FFT FFT FFT 3. FFT active Ctrl+A Process FFT Inverse FFT (1) Inverse FFT of (2) 2-1 FFT FFT 4.

7 FFT FFT FFT 5. FFT FFT (2) ( 3) (i) -7 2-2 A-21 6.

8 (i) -7 FFT FFT 7. Pleurosigma 2-3 FFT We b Dr. Roderich Zeiss The Microscope and Its Revelations. 7. Auflage, London 1891 Web Analyze Measure Image Adjust Size Image Cut, Copy, Invert .. Process Image Calculator Process Math Process Smooth, Enhance Contrast, Sharpen, Noise.

9 Plugins Macro ~kam/LM/ A-22 iii Image J *.AVI Frame Image J File Import AV OK 200 First Frame Last Frame Convert to Grayscale 1. Image Stacks Start Animation xy z Stacks x/y /t 2.

10 Active Image Stacks Z Project xy z a. 8bit-Stacks 32bit-Stacks Image Type 32-bit 256 32-bit = 4,294,967,295 32-bit A-23 AVI Convert to Grayscale b. Image Stacks Z Project Projection Type Sum Slices Sum Slices 32-bit t (iii) -4 Analyze Histogram Image Adjust Brightness/Contrast 8bit 0 28 32-bit 0 232 3.