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Bioreactors and Fermentors— Powerful Tools for Resolving ...
liters of a bacteria or yeast culture in shake flasks are feasible. But if dozens or even hundreds of flasks are needed to produce the required amount of biomass, lab space becomes limiting and the amount of manual work explodes. Stirred-tank bioreactors are scalable, meaning they allow increasing the size of the cultivation vessel instead of the
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Bioprocessing Basics - Eppendorf
www.eppendorf.comcultivation bottlenecks. Introduction Many applications are well served by the cultivation of bacteria or yeast in shake flasks and cells in dishes or T-flasks. Bioreactors and fermenters, however, improve productivity and save work, time, and lab space for scientists, who: • need large quantities of cells, microbes, or the products they express
Cell Thawing Protocol Standardization – Guide for More ...
www.eppendorf.comthawing process in a reproducible procedure, which offers important benefits in situations where there is a higher de-mand for standardization and documentation of the thawing process. They are also well suited for situations where water baths are impractical [6,7]. The most important drawback to dedicated thawing devices is the upfront investment
A Beginner’s Guide to Bioprocess Modes – Batch, Fed- Batch ...
www.eppendorf.com[2]. This strain has an ampicillin resistance gene (bla) encoded on a plasmid. A concentration of 0.1 g/L ampicillin must be maintained in all cultures, since absence or low concentrations of ampicillin will result in plasmid loss [3]. Therefore, from inoculum preparation in shake flasks to fermentation runs in 3 L vessels, we always added 0.1 g/L
BioBLU c and BioBLU p Single-Use Vessels for cell culture
www.eppendorf.comLeachables and extractables are defined as substances that can either be extracted from plastic materials under harsh conditions (e.g. extreme temperatures, presence of solvent) or released spontaneously under standard conditions. They may originate from compounds used in polymer manufacturing such as softeners, stabilizers, and antioxidants. Espe-
Centrifuge 5804/5804 R Centrifuge 5810/5810 R - Eppendorf
www.eppendorf.com2.3.1 Declaration concerning the ATEX directive (2014/34/EU) Due to its design and the environmental conditions inside the device, the Centrifuge 5804/5804 R/5810/ 5810 R is not suitable for use in a potentially explosive atmosphere. The device may only be used in a safe environment, such as in the open environment of a ventilated
Scale-Up of Escherichia coli Fermentation from Small Scale ...
www.eppendorf.comPower number (Np) The (impeller) power number (also known as Newton number) is a dimensionless number associated with different type of impellers. Np is commonly used to calculate impeller power consumption during bioprocess scale-up [2]. We determined the power number and P/V values at various Used with 1 L fermentation glass vessel Used with 10 L
Cell Culture Scale-Up in Stirred-Tank Single-Use Bioreactors
www.eppendorf.comThe stirred-tank bioreactor design is relatively easy to describe with classical engineering approaches (Figure 1). Parameters to describe this vessel geometry include impeller diameter, vessel diameter, liquid height, and ratios thereof. It is the bioreactor design for which most research on Figure 1: Stirred-tank bioreactor design d D i h ...
Eppendorf Tubes
www.eppendorf.comWARNING! Damage to health from toxic, radioactive or aggressive chemicals as well as infectious liquids and pathogenic germs. Observe the national regulations for handling these substances, the biological security level of your laboratory, the material safety data sheets and the manufacturer's application notes.
Centrifugation Operating Manual - Eppendorf
www.eppendorf.comRead this operating manual completely before using the device for the first time. Observe the instructions for use of the accessories where applicable. This operating manual is part of the product. Please keep it in a place that is easily accessible. Enclose this operating manual when transferring the device to third parties.
Centrifuge 5425 - Eppendorf
www.eppendorf.comOperating instructions Centrifuge 5425 English (EN) 1 Operating instructions 1.1 Using this manual Read this operating manual completely before using the device for the first time. Observe the instructions for use of the accessories where applicable. This operating manual is part of the product. Please keep it in a place that is easily accessible.
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science.umd.eduLAB 4. Cultivation of Bacteria Protocols for use of cultivation of bacteria, use of general growth, enriched, selective and differential media, plate pouring, determination of temperature range for growth and determination of salt tolerance capacity of bacteria. INTRODUCTION In nature, bacteria exist as mixed populations.
Isolation &Characterization of Bacteria - G-Biosciences
www.gbiosciences.comAn interesting hands ron lab activity that teaches students the skills required for the isolation of bacteria from test samples. This kit teaches aseptic handing techniques and cultivation of bacteria. Using theses bacterial culture techniques students discover and isolate the bacteria present around us.
Bioprocessing Basics - Eppendorf
www.eppendorf.comcultivation bottlenecks. Introduction Many applications are well served by the cultivation of bacteria or yeast in shake flasks and cells in dishes or T-flasks. Bioreactors and fermenters, however, improve productivity and save work, time, and lab space for scientists, who: • need large quantities of cells, microbes, or the products they express
PACKAGE OF PRACTICES FOR CULTIVATION OF FRUITS
www.pau.eduFOR CULTIVATION OF FRUITS Compiled and Edited by Dr. Surinder Kumar Thind ... acid bacteria @ 5.0% (w/v)) by incubating at 37ºC for 24 hrs. Indian ... starter culture (consortia of ten LAB strains @ 5% w/v) and fermenting till 48 hrs at 37°C in tightly stoppered sterilized glass jar. 1 1. INTRODUCTION Presently, total area under fruit crop is ...
SELECTIVE AND DIFFERENTIAL MEDIA - Youngstown State …
crcooper01.people.ysu.educulture media for the cultivation and identification of enteric organisms and is also used in the isolation of pathogens from foods and coliforms in water samples. Like HE agar, MAC incorporates bile salts, in addition to sodium chloride, which inhibits the growth of most Gram-positive bacteria. Differentiation of enteric microorganisms
PURE CULTURE TECHNIQUES I. OBJECTIVES
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Lab Exercise 3: Media, incubation, and aseptic technique
faculty.sdmiramar.eduCultivation chambers called incubators are used to grow microbes at a range of temperatures. In this lab, we have set incubators for two temperatures most commonly used as optimum temperatures by the microbes we work with: 25° C and 37° C. We also have an incubator set at 4° C (aka a refrigerator!) that
Nutrient Agar - himedialabs.com
himedialabs.comNutrient Agar is used as a general purpose medium for the cultivation of less fastidious microorganisms, can be enriched with blood or other biological fluids. Composition** Ingredients Gms / Litre Peptone 5.000 Sodium chloride 5.000 HM peptone B# 1.500 Yeast extract 1.500 Agar 15.000 Final pH ( at 25°C) 7.4±0.2
Bacterial Culture & Growth - G-Biosciences
www.gbiosciences.comStudents can only see three phases of bacteria growth (lag, log and stationary) during one day culture. The bacteria growth will enter decline phase within 24 hours of culture. As the lysis of dead bacteria is slow, the absorbance of the total bacteria mass …