식물바이러스와 식물생명공학 - biosafety.or.kr

1 RNA DNA ( 1). , , , , , 1,500.

2 , (transcrip-tion promoter), (transcription terminator), (translation enhance) . , (Cauliflower mosaic vi-rus, CaMV) 35S ' , . 1 (20~30nm) (400~500nm) (700~900nm) UTILIZATION OF PLANT VIRUS AS A TOOL FOR PLANT BIOTECHNOLOGY 026027 LMO INFORMATION | LMO.

3 , ( 3).. , polyprotein PTC , ( 4). , . SMV 5 3 Poly A tailTranslation6K16K2P1HC-proP3 ClVPgNlbCPNla-proP1HC-pro6k16k2 P3 ClVPgNlbCPNla-proP1HC-proP3 ClVPgNlbCPNla-proPost-translational cleavageCleavage by Nla-pro6k16k2 SMV SMV P1 HC-Pro 5 3 Poly A tailP1HC-proP3 ClVPgNlbCPNla-proP1in clsNla-proin transDIQHY/SRTRESVSLQ/SQNPEXbalMlul SMV (GFP) SMV-GFPP1HC-proP3 ClVPgNlbCPNla-proNAGFP SMV SMV-BARP1HC-proP3 ClVPgNlbCPNla-proNABARBAR.

4 SMV-Dual5 P1HC-proP3 ClVPgNlbCPNla-proNAP1in clsNla-proin transDIQHY/SRTRESVSLQ/SQNPEXbalMlulNla-p roin transNla-proin transESVSLQ/SVDYVESVSLQ/SGKESaASnaBISMV- GFP+nCFPCFP nCFP GFP GFPL ightMergeP1HC-proP3 ClVPgNlbnCFPCPNla-proNAGFPnCFP : Nuclear localization signal CFP SMV SMV-BAR 2 (SMV) SMV 5 3 Poly A tailTranslation6K16K2P1HC-proP3 ClVPgNlbCPNla-proP1HC-pro6k16k2 P3 ClVPgNlbCPNla-proP1HC-proP3 ClVPgNlbCPNla-proPost-translational cleavageCleavage by Nla-pro6k16k2 SMV SMV P1 HC-Pro 5 3 Poly A tailP1HC-proP3 ClVPgNlbCPNla-proP1in clsNla-proin transDIQHY/SRTRESVSLQ/SQNPEXbalMlul SMV (GFP) SMV-GFPP1HC-proP3 ClVPgNlbCPNla-proNAGFP SMV SMV-BARP1HC-proP3 ClVPgNlbCPNla-proNABARBAR.

5 SMV-Dual5 P1HC-proP3 ClVPgNlbCPNla-proNAP1in clsNla-proin transDIQHY/SRTRESVSLQ/SQNPEXbalMlulNla-p roin transNla-proin transESVSLQ/SVDYVESVSLQ/SGKESaASnaBISMV- GFP+nCFPCFP nCFP GFP GFPL ightMergeP1HC-proP3 ClVPgNlbnCFPCPNla-proNAGFPnCFP : Nuclear localization signal CFP SMV SMV-BAR 3 SMV 5 3 Poly A tailTranslation6K16K2P1HC-proP3 ClVPgNlbCPNla-proP1HC-pro6k16k2 P3 ClVPgNlbCPNla-proP1HC-proP3 ClVPgNlbCPNla-proPost-translational cleavageCleavage by Nla-pro6k16k2 SMV SMV P1 HC-Pro 5 3 Poly A tailP1HC-proP3 ClVPgNlbCPNla-proP1in clsNla-proin transDIQHY/SRTRESVSLQ/SQNPEXbalMlul SMV (GFP) SMV-GFPP1HC-proP3 ClVPgNlbCPNla-proNAGFP SMV SMV-BARP1HC-proP3 ClVPgNlbCPNla-proNABARBAR.

6 SMV-Dual5 P1HC-proP3 ClVPgNlbCPNla-proNAP1in clsNla-proin transDIQHY/SRTRESVSLQ/SQNPEXbalMlulNla-p roin transNla-proin transESVSLQ/SVDYVESVSLQ/SGKESaASnaBISMV- GFP+nCFPCFP nCFP GFP GFPL ightMergeP1HC-proP3 ClVPgNlbnCFPCPNla-proNAGFPnCFP : Nuclear localization signal CFP SMV SMV-BAR 4 (Molecular farming) , (gene deliv-ery vector).

7 , . , (Soybean mosaic virus, SMV) , open reading frame(ORF) polyprotein (post-translational cleavage, PTC) ( 2). , PTC ( 2). NANOMACHINES THAT PRODUCEPROTEIN0280292000 . (Gene silencing).

8 RNA RNA . 2006 (Andrew Fire Craig Mello) . , , ( 5). , . , (VIGS:Vi-rus-Induced Gene Silencing) , VIGS ( 5).

9 , GMO .. , , Gain of function Loss of function .. Bean pod mottle virus-based VIGS vector Silencing of soybean PDS geneCo-ProRNA1 HELProRdRpTransgeneTarget RNDcleavageNA5 Transcript5 Double-stranded RNAsiRNA duplexRNA PolRISCD icerVIGST arget RNAcleavage5 Virus vector carryingplant gene sequenceReplication5 Replication intermediatesiRNA duplexRISCD icerMPRNA2 LCPSCPNA partial PDSBamHl 5 VIGS UTILIZATION OF PLANT VIRAL

10 VECTOR PROSPECTS030031