BIOTECHNOLOGY AND ITS APPLICATIONS

1 82 biology , EXEMPLAR PROBLEMSCHAPTER 12 BIOTECHNOLOGY AND ITSAPPLICATIONSMULTIPLE-CHOICE cotton is GM bacterial gene expressing to all of human insulin part of mature insulin for formation of disulphide during maturation of pro-insulin to for its biological stands Engineering Action Environment Action Engineering Approval and Environment Approval committee4. -1 antitrypsin to treat to treat emphysema20/04/2018 UNIT II: STRUCTURAL ORGANISATION IN PLANTS AND ANIMALSUNIT II: STRUCTURAL ORGANISATION IN PLANTS AND ANIMALBIOTECHNOLOGY AND ITS probe which is a molecule used to locate homologous sequences in amixture of DNA or RNA molecules could RNA or be ssDNA but not the correct option regarding RNA virus that synthesises DNA during DNA virus that synthesises RNA during ssDNA dsRNA site of production of ADA in the body protoxin primitive denatured produced by is of physiology of of normal physiology of of altered physiology of of the trigger for activation of toxin of Bacillus thuringiensis pH of pH of action in the insect gut20/04/201884 biology , EXEMPLAR rice variety of rice grown along the yellow river in stored rice having yellow colour transgenic rice having gene for - variety of rice with yellow coloured RNAi.

2 Genes are silenced first clinical gene therapy was done for the treatment (Severe Combined Immuno Deficiency resulting formdeficiency of ADA) is an enzyme which is deficient in a genetic disorder SCID. What isthe full form of ADA? of a gene could be achieved through the use RNA RNAi and antisense of the aboveVERY SHORT ANSWER TYPE view of the current food crisis, it is said, that we need another greenrevolution. Highlight the major limitations of the earlier green GMO. How is it different from a hybrid? between diagnostics and therapeutics. Give one exampleand for each II: STRUCTURAL ORGANISATION IN PLANTS AND ANIMALSUNIT II: STRUCTURAL ORGANISATION IN PLANTS AND ANIMALBIOTECHNOLOGY AND ITS the full form of ELISA. Which disease can be detected using it?Discuss the principle underlying the a disease be detected before its symptoms appear?

3 Explain theprinciple a short note on Biopiracy highlighting the exploitation of developingcountries by the developed proteins are secreted in their inactive form. This is also true ofmany toxic proteins produced by micro organisms. Explain how themechanism is useful for the organism producing the toxin? creating genetically modified organisms, genetic barriers are notrespected. How can this be dangerous in the long run? has the Indian Parliament cleared the second amendment of thecountry s patents bill? any two reasons why the patent on Basmati should not have goneto an American was Insulin obtained before the advent of rDNA technology? Whatwere the problems encountered? respect to understanding diseases, discuss the importance oftransgenic animal the first transgenic cow. Which gene was introduced in this cow?

4 Is a useful tool for early diagnosis of an infectious disease. is GEAC and what are its objectives? which variety of Indian rice, the patent was filed by a USA Company? the advantages of ANSWER TYPE expression can be controlled with the help of RNA. Explain themethod with an our traditional knowledge can we prove costly in the area ofbiological patenting. any four areas where genetic modification of plants has , EXEMPLAR is a recombinant DNA vaccine? Give two is it that the line of treatment for a genetic disease is different frominfectious diseases? briefly how a probe is used in molecular was the first patient who was given gene therapy? Why was thegiven treatment recurrent in nature? examples under each category, discuss upstream anddownstream Antigen and Antibody. Name any two diagnostic kits basedupon technique is based on the principles of antigen-antibodyinteraction.

5 Can this techique be used in the molecular diagnosis of agenetic disorder, such as phenyketonuria? is a mature, functional insulin hormone different from itsprohormone form? therapy is an attempt to correct a genetic defect by providing anormal gene into the individual. By this the normal function can berestored. An alternate method would be to provide the gene product(protein/enzyme) known as enzyme replacement therapy, which wouldalso restore the function. Which in your opinion is a better option? Givereason for your animals are the animals in which a foreign gene is animals can be used to study the fundamental biological process,phenomenon as well as for producing products useful for mankind. Giveone example for each a foreign DNA is introduced into an organism, how is it maintainedin the host and how is it transferred to the progeny of the organism?

6 Cotton is resistant to pest, such as lepidopteron, dipterans andcoleopterans. Is Bt cotton also resistant to other pests as well?LONG ANSWER patient is suffering from ADA deficiency. Can he be cured? How? transgenic animals. Explain in detail any four areas where theycan be II: STRUCTURAL ORGANISATION IN PLANTS AND ANIMALSUNIT II: STRUCTURAL ORGANISATION IN PLANTS AND ANIMALBIOTECHNOLOGY AND ITS have identified a useful gene in bacteria. Make a flow chart of thesteps that you would follow to transfer this gene to a five areas where BIOTECHNOLOGY has influenced our are the various advantages of using genetically modified plants toincrease the overall yield of the crop? with the help of one example how genetically modified plantscan:(a)Reduce usage of chemical pesticides(b)Enhance nutritional value of food the disadvantages of insulin obtained from the pancreas ofslaughtered cows and the advantages of recombinant is meant by the term bio-pesticide?

7 Name and explain the mode ofaction of a popular the five key tools for accomplishing the tasks of recombinant DNAtechnology. Also mention the functions of each