Bone Marrow Aspirate Concentrate - Nupsala

1 The Importance of a Diverse Cell PopulationAll of the cells found in the nucleated cell fraction of a Marrow Aspirate play a role in tissue regeneration. The most important factor in cell therapy augmented tissue repair is to deliver this mixed population of cells in the proper micro-environment of the injury site to establish easy penetration of nutrition, oxygen tension and tensile strength. The cooperation of different kinds of cells supports proliferating and differentiating young cells through the continuous synthesis of growth factors. VasculogenesisMammals naturally respond to tissue trauma by mobilizing stem cells and supporting nucleated cells from the Marrow space, through the peripheral blood where they Concentrate themselves around the site of injury. The more efficient the body is at this stem cell mediated response in the first week after an injury, the better the patient fares.

2 Simply aspirating Marrow , concentrating the nucleated cell fraction and delivering that biologic to the injury site exactly mimics and enhances the body s natural reparative of Stem Cells Found in MarrowBone Marrow contains four general sub groups of stem cells: Mesenchymal stem cells (MSC) Endothelial progenitor cells (EPC) Hematopoietic stem cells (HSC) Stem cells for which cell markers do not exist but are known to be present based on indirect tests of clinical effect. These mixed cell populations grow in vivo easily without mutual suppression and work together as feeder cells to each other. bone Marrow Aspirate Concentrateas a TherapyHarvest leverages the therapeutic potential of bone Marrow stem cells by producing a mixed population of stem and early stage progenitor cells that are capable of having a dramatic therapeutic key advantage Harvest provides is that the number of multi-potent stem, progenitor, and other regenerative cells are significantly concentrated in the patient s own physiological plasma in a point-of-care procedure within 15 to Cultured Cells Safer and more affordable9.

3 10 Clinically more effective on cell for cell basis8 Have greater cell diversity and can be delivered in greater numbers8 Growth FactorsThe early stages of the healing cascade are primarily mediated by platelets and the release of cellular growth factors that control Growth Factors(PDGF) Initiates connective tissue healing Increases mitogenesis, angiogenesis, and vasculogenesisVascular Endothelial Growth Factors(VEGF) Possess potent angiogenic, mitogenic, and vascular permeability enhancing activitiesTransforming Growth Factor(TGF) Increases chemotaxis and mitogenesis Stimulates deposition of collagenHealing CascadeThe body s response to injury is a very specific and well documented series of steps collectively referred to as the Healing Cascade . This cascade is comprised of several distinct phases which rely on an integrated cellular systems approach that combines the use of Growth Factors, supporting cells and Stem Cells to create the optimal environment for tissue use of these bioactive cells is to exactly mimic and augment the natural healing process and represents a new frontier of clinical treatment.

4 Autologous Platelet Concentrate APC+ is a specific concentration of platelets and plasma that can be directly delivered to an injury to supplement exactly the body s natural conditions to reduce scar formation and stimulate more complete the injury continues to remodel, healing progresses by attracting stem cells from the periphery into the affected Platelet Concentrate APC+ contains high concentrations of growth factors within alpha-granule releasate. These growth factors initiate a cascade that results in chemoattraction of circulating stem Platelet Concentrate 2010 Harvest Technologies Corp. Printed in the USA. Harvest, SmartPrep, BMAC, APC+, SmartJet, Accelerating Healing Naturally are registered trademarks of Harvest Technologies 050-02-0762