ChemiDoc Touch Imaging System User Guide

1 ChemiDoc Touch Imaging System with Image Lab Touch SoftwareUser GuideVersion Touch Imaging Systemwith Image Lab Touch SoftwareUser GuideVersion Technical SupportThe Bio-Rad TechnicalSupport department in the isopen Mondaythrough Friday, 5:00 AM to 5:00PM, Pacifictime. Go worldwide : 1-800-424-6723, option only)For technicalassistance outside the and Canada, contact your localtechnicalsupport part of thispublication maybe reproduced or transmitted in anyform or byanymeans, electronicormechanical, including photocopy, recording, or anyinformation storage or retrievalsystem, withoutpermission in writing from reservesthe right to modifyitsproductsand servicesat anytime. Thisuser Guide issubject tochange without notice. Although prepared to ensure accuracy, Bio-Rad assumesno liabilityfor errorsoromissions, or for anydamage resulting from the application or use of Fluor, Coomassie Fluor, Qdot, SYBR, and SYPROare trademarksof Life TechnologiesCorporation.

2 Coomassie isa trademarkof BASF Aktiengesellschaft. Cy2 isa trademarkof GE and Krypton are trademarksof Thermo Fisher ScientificInc. Excel, PowerPoint, and Windowsaretrademarksof Microsoft Corporation. Macand Numbersare trademarksof Apple Inc. GelGreen andGelRed are trademarksof Biotium, Inc. Mitsubishiisa trademarkof PlusProtein standardsare sold under license from Life TechnologiesCorporation, Carlsbad, CA,for use onlybythe buyer of the product. The buyer isnot authorized to sellor resellthisproduct or Stain Free gelsare covered Pat. No. 7,569, Laboratories, Inc. islicensed byInvitrogen Corporation to sellSYPRO productsfor research useonlyunder Patent Number 5,616, 2015 byBio-Rad Laboratories. of ContentsSafety and Regulatory Compliance7 Important Safety Information7 Warranty8 General Precautions9 Regulatory Notices9 Alert Icons10 Instrument Safety Warnings11 Notice12 Power Safety Information13 Voltage Setting Information13 Fuses13 Chapter 1 Introduction15 Product Features15 Front Panel Components17 Rear Panel Components18 CCD Camera and Lenses18 Introduction to Image Lab Touch Software18 Emission Filters19 Optional Accessories19 Printer19 White Sample Tray19 User Guide | iiiTable of ContentsBlue SampleTray19 Supported Tray Types20 Technical Specifications21 Environmental Requirements23 Chapter 2 Image Lab Touch Software Overview25 Starting Image Lab Touch25 Setting the System Date and Time26 Touch Screen Actions29 The Application Interface29 About the Live

3 View Screen31 The Image Lab Touch Main Toolbar32 Tabs33 About the Image View Screen34 The Image View Toolbar36 The Gallery36 Gallery Screen Components38 Getting Help38 Entering Text on Screen39 Using the On-Screen Keypad39 Using the On-Screen Keyboard40 Editing the Welcome Screen40 Setting the Sound Volume42 Chapter 3 Acquiring an Image43 About Sample Trays43 Workflow for Acquiring a Chemiluminescent Image46 Specifying the Image Size46 Choosing the Application48iv | ChemiDocTouch Imaging SystemTable of ContentsAbout Exposure Times for Chemiluminescent Images48 The Image Resolution/Sensitivity Scale49 Specifying the Region of Interest for Auto Exposure54 Setting the Exposure Time for a Chemiluminescent Image56 Setting the Exposure Automatically56 Setting the Exposure Manually58 Signal Accumulation Mode59 Configuring Signal Accumulation Mode60 Acquiring Images60 Workflow for Acquiring Gel and Blot Images62 Specifying the Image Size63 Choosing the Application63 Setting the Exposure Time64 Acquiring an Image65 Deleting Images66 Chapter 4 Viewing Images67 About Image View69 About the Gallery70 Viewing Images in Image View71 Selecting and Opening Images in the Gallery72 Adjusting How Images Are Displayed75 Light Intensity Histogram75 Restoring Default Settings78 Checking Intensity Values79 About Merging Images80 Comparing Images81 Searching for Images in the Gallery84 User Guide | vTable of ContentsPrinting Images84 Merging Images85 Naming Merged Images86 Renaming Images87 Deleting Images88 Viewing Image Information89 Adding Notes to an

4 Image90 Chapter 5 Excising Bands91 Excising Bands from Samples91 Excising Bands on a Chemi/UV/Stain-Free Tray91 Excising Bands on a White or Blue Tray97 Chapter 6 Exporting Images99 Exporting to a USB Flash Drive or External Hard Drive100 Exporting to a Shared Folder101 Disconnecting from a Shared Folder103 Appendix A Maintaining the Imaging System105 Updating Image Lab Touch Software105 Reinstalling Image Lab Touch105 About the UV Transilluminator Bulbs106 When Transilluminator Bulbs Fail106 Replacing the UV Transilluminator Bulbs107 Replacing the Fuses109 Appendix B Ordering Information113 Appendix C Exporting to a Shared Folder117vi | ChemiDocTouch Imaging SystemSafety and Regulatory ComplianceImportant Safety InformationPlease read these instructions before operating the ChemiDoc instrument is suitable for research use only.

5 Therefore, it must be used only byspecialized personnel who know the health risks associated with the reagents that arenormally used with this :The Imaging of some applications involves UV instrument should be used only by trained personnel who know thehealth risks associated with the UV radiation normally associated with thisinstrument. Users should be trained on the appropriate personal protectiongear for working with UV light to minimize UV perform band excision using the Chemi/UV/Stain-Free tray, the transilluminator draweris pulled out with the UV source enabled. This exposes the user to UV radiation, which cancause permanent damage to the eyes and skin. In its lowered position, the instrument sacrylic shield provides UV protection. However, in its raised position, it does not providecomplete protection to the user, and it does not protect others who are standing in the areaaround the Guide | 7 Safetyand RegulatoryComplianceTo protect users and bystanders, these procedures must be followed:nProtect all skin surfaces (including the neck, ears, and hands).

6 Before performing bandexcision, the user and anyone near the imager must put on protective gear includingUV protective safety glasses, a face shield, lab coat, and gloves. A typical andreasonable expectation of use is three operations per user a day for three without protective gear must stand at least meters (five feet) away fromthe imager and limit their exposure to no more than one hour per :There is no exposure to UV radiation with the blue or white trays. No protectivegear is necessary when excising bands with these ChemiDoc Touch Imaging System is warranted against defects in materials andworkmanship for one year. If any defect occurs in the instrument during this warrantyperiod, Bio-Rad Laboratories, Inc. will repair or replace the defective parts at its discretionwithout charge.

7 The following defects, however, are specifically excluded:nDefects caused by improper operationnRepair or modification done by anyone other than Bio-Rad Laboratories, Inc. or thecompany s authorized agentnUse of spare parts supplied by anyone other than Bio-Rad Laboratories, caused by accident or misusenDamage caused by disasternCorrosion caused by improper solvents or samples8 | ChemiDocTouch Imaging SystemGeneralPrecautionsGeneral PrecautionsnRead the user Guide the instrument only for the intended purpose of gel and blot image acquisition inresearch the instrument to a grounded power source and to a circuit not pour liquids on or inside the the sample tray after NoticesThe ChemiDoc Touch Imaging System is designed and certified to meet EN 61010, theinternationally accepted electrical safety standard, EMC regulations, and TUVrequirements.

8 Certified products are safe to use when operated in accordance with thisuser Guide . Do not modify or alter this instrument in any way. Modification or alteration ofthis instrument will:nVoid the manufacturer s warrantynVoid the regulatory certificationsnCreate a potential safety hazardCaution:Bio-Rad Laboratories, Inc. is not responsible for any injury ordamage caused by use of this instrument for purposes other than thosefor which it is intended or by modifications of the instrument notperformed by Bio-Rad Laboratories, Inc., or an authorized Guide | 9 Safetyand RegulatoryComplianceAlert IconsAlert icons call attention to caution and warning paragraphs. The icon indicates the type ofhazard a potential hazard requiring special attention. This icon isused when the hazard or condition is of a general HazardIndicates a potential hazard requiring special attention when you areworking with electricity or electrical heat and flammable materialsIndicates a potential hazard requiring special attention when you areworking with extreme heat and flammable hazardIndicates a potential hazard requiring special attention when you areworking with UV 1.

9 How alert icons are usedCautionsA caution alerts you to take or avoid a specific action that could result in loss of data ordamage to the instrument. A caution can also indicate that, if the precaution against apotential hazard is not taken, minor or moderate injury might | ChemiDocTouch Imaging SystemInstrument SafetyWarningsExampleCaution:With the exception of cleaning or replacing light bulbs, refer allservicing to qualified Bio-Rad personnel or their warning precedes an action that, if not followed correctly, could cause serious injury ordeath to the operator, serious or total loss of data, or serious damage to the !Keep the UV shield open for as little time Safety WarningsBefore you operate the instrument, carefully read the contents of Table Guide | 11 Safetyand RegulatoryComplianceIconExplanationCauti on:With the exception of cleaning orreplacing light bulbs, refer all servicing toqualified Bio-Rad personnel or their agents.

10 If youexperience technical difficulties with theinstrument, contact Bio-Rad to schedule instrument should not be modified or alteredin any way. Alteration voids the manufacturer swarranty and might create a potential safetyhazard for the !If the tray interlock is defeated, thereis a possibility of UV-B radiation hazard due toUV-B light exposure. Exercise caution whenservicing the !This instrument must be connected toan appropriate AC voltage outlet that is 2. Safety cautions and warnings for the instrumentNoticeThe ChemiDoc Touch instrument is intended for laboratory use only. This device is meantfor use by specialized personnel who know the health risks associated with reagents usedin electrophoresis. The UV light source is computer controlled, and proper interlocks areimplemented to avoid users accidental exposure to UV radiation.