ChemiDoc Touch MP イメージングシステム - Bio …

1 Western BlottingChemiDoc Touch MP Fluorescence and chemiluminescence detection without 140-0002 2-2-24 TEL 03-6361-7000 FAX 03-5463-8480 532-0025 1-14-11 TEL 06-6308-6568 FAX 06-6308-3064 812-0013 2-5-28 TEL 092-475-4856 FAX 092-475-4858 TEL 03-6404-0331 FAX 03-6404-0334 2018 11 C11178L 1811c 2 StarBright B700 CCD Export U SB LAN Export E xport T I F F JPEG

2 Live View UV UV ChemiDoc Touch MP Blot/UV/Stain-Free White Blue UV , Ethidium bromide, SYPRO Ruby, Oriole,GelRed, SYBR TGX Stain-free CBB, Silver, Zinc, Copper Gel Green, SYBR UV U V 31 NO COMPROMISESMORE THAN TRADITIONAL WESTERN BLOTTING 10 secChemiluminescence20 secStarBright Blue 700 FluorophoreANDAND200 secIR800 Fluorescence1 ChemiDoc Touch MP / CCD 10 sec200 sec ChemiDoc Touch (MP)

3 ChemiDoc Touch MP : 259 dpi : 186 dpi cm7.

4 6 c cm10 cm7 c m : 600 3x3 A BETTER WAY TO WESTERN BLOT 4 ChemiDoc Touch MP

5 HeLa EGF ERK1/2 : TubulinERK1/2 EGF(ng/ml)0 . ERK1/2 Mouse mAb IgG StarBright Blue 700 Anti-total ERK1/2 Rabbit mAb IgG StarBright Blue 520 hFAB RhodamineA BETTER WAY TO WESTERN BLOT 5 BlueGreenRedFar RedNearInfrared 400900 DyLight 488 Alexa Fluor 488 Cy2 StarBright 520 DyLight 550 Alexa Fluor 546 Cy3 Rhodamine StarBright 700 DyLight 680 Alexa Fluor 680 IRDye 680 DyLight 800 Alexa Fluor 790 Cy7 IRDye 800 DyLight 650 Alexa Fluor 647 Cy5 SYPRO RubyWavelength.

6 NmBio-Rad ChemiDoc Touch MP CCD ChemiDoc Touch MP Blue Green Red CCD Far Red Near Infrared StarBright ChemiDoc Touch MP StarBright Blue ChemiDoc Touch MP 470 nmExcitationLight-absorbingmonomerEnergy transfermonomerLight-emittingmonomerLink er monomer forfunctionalizationEnergy transferEmission700 nmStarBright Blue 700 Light-absorbing monomer 470 nm Energy transfer monomer Energy transfer monomer Light-emitting monomer 700 nm StarBright Blue 700 : 10 secStarBright Blue 520 : 3 secIR800 : 300 secAlexa 488 : 35 secClarity Max Western ECL Substrate : 8 secDylight 488.

7 35 secFcFabMonovalent FabStarBright Blue 2 HKP hFAB Rhodamine StarBright Blue HuCAL HKP HKP Fc 2 StarBright Blue 123451234512345123451234512345 A BETTER WAY TO WESTERN BLOT 1 Lane 2 Lane 3 Lane 4 Normalized band intensityLane 5 Lane 6 Lane 7 Lane Touch30 secFilm30 secA456456X- ChemiDoc Touch : E.

8 Coli cell lysate g 2x : LaclB. ChemiDoc Touch X - ChemiDoc Touch X- ChemiDoc Touch (MP) X- ChemiDoc Touch X- ChemiDoc Touch X- X- 4-6 ChemiDoc Touch ChemiDoc Touch X- Film30 secChemiDoc Touch30 secX- ChemiDoc Touch .

9 University of California, San Francisco, ChemiDoc Touch X- X- ChemiDoc Touch A BETTER WAY TO WESTERN BLOT 7123 Imager C 15 sec exposureImager A 15 sec exposureImager B 15 sec exposure1233 x x 1072 x x 1071 x 107 5 x 1060 Band intensity ChemiDoc Touch Imager A Imager B Imager C ChemiDoc Touch MP : Jurkat Cell Lysate 10 g 2x.

10 P44/42 MAPK Erk1/2 ChemiDoc Touch Imaging System 15 sec exposure Clarity Clarity Max 2 Clarity Clarity Max Clarity Clarity Western ECL SubstrateChemiDoc Touch MP CCD Clarity Max Western ECL SubstrateClarity Western ECL Substrate Low-fg Mid-fg 2 2 CCD X PVDF PVDF 38,000(100 ml) 20,000(200 ml) Clarity Max Western ECL Substrate( )Purified IKB alpha, pg 7 00 470 310 210 140 90 60 40 30 Clarity MaxG SG PT FT DSignal-to-noise ratio2520151050 40 30 Protein per well, pgNDClarity MaxG ST FClarity Western ECL Substrate( )