Chromatography Columns and Supplies - Hichrom: the …

1 HICHROMC hromatography Columns and SuppliesHichrom Limited1 The Markham Centre, Station RoadTheale, Reading, Berks, RG7 4PE, UKTel: +44 (0)118 930 3660 Fax: +44 (0)118 932 3484 Email: 9LC COLUMNSK romasilLC Columns Tel: 0118 930 3660141 hichrom LimitedKromasilKROMASIL Spherical porous high purity silica Analytical to preparative particle sizes Robust and reproducible 300 and chiral phases availableThe Kromasil range of products is based on a highly recommended, robust, pure silica manufactured by Eka Chemicals AB of Sweden (now known as Akzo Nobel Pulp and Performance Chemicals).

2 The Kromasil classic phases are designed to meet the highest demand in HPLC, SFC and SMB from analytical to process scale and are available in six particle sizes, ranging from to 16 m. Kromasil Eternity is a newer platform with a grafted organosilane surface for extended pH stability (2 -12) and is available in and 5 m particle sizes (see pages 150 and 151). For small pore phases, see pages 141-147. For wide pore phases, see page 148. For chiral phases, see pages Classical PhasesBatch to Batch ReproducibilityKromasil material is manufactured using the well-known sol-gel technique which results in a silica with optimised chemical and mechanical stability, optimised pore structure and reproducible surface area.

3 Development and production of the Kromasil packings are ISO 9001:2000 certified. Extensive control and testing is performed throughout the whole production process, ensuring the highest possible reproducibility. Proof of this can be seen in Figures 1 and 2, which show the reproducibility of batches of Kromasil. Kromasil packings for process scale HPLC are manufactured in batches of over 100kg, within a modern plant whose capacity is several tonnes per : 250 x mmEluent: CH3CN - H20 (70:30)Flow rate: 2ml/minFigure 1.

4 Batch to batch reproducibility of 50 batches of Kromasil C18 with respect to k and aColumn: 250 x mmEluent: CH3CN - H20 (70:30)Flow rate: 2ml/minFigure 2. Batch to batch reproducibility of 50 batches of Kromasil C18 with respect to BET and ligand densityKromasil PhaseFunctional GroupEndcappedParticle Size ( m)Pore Size ( )Surface Area (m2/g)Carbon Load (%)Silica--5, 7, 10, 13, , 5, 7, 10, 13, , 5, 7, 10, 13, , 5, 7, 10, 13, , 5, 7, 10, 13, , 5, 7, 10, 13, , 10, , 10, , 10, , 10, 16300110-C4 ButylYes5, 10, , 10, , 10, Due to its well optimised manufacturing process, the endcapping step, in this case, has no effect on the (m2/g)

5 Ligand density ( mol/m2)BET-areaLigand (butylbenzene/propylbenzene)k (butylbenzene)LC Columns Kromasil142 For free technical advice and support email (continued)Chemical StabilityAt low pH the major mechanism of column degradation is acid hydrolysis of the bonded phase. The nature of the surface silanols and the coating density of the monofunctional silane are key parameters. In Figure 3 Akzo Nobel demonstrate the excellent chemical stability of Kromasil C8 pH 7 or higher, the major mechanism of degradation is dissolution of silica in aqueous eluents.

6 The mode of manufacture of the silica, its surface treatment and the coating density of the silane, all affect higher pH s stability in alkaline conditions was tested at ambient temperature by eluting a Kromasil 10-C8 column (200 x ) with CH3OH NH4Ac/NH3, pH (70:30) at a flow rate of for a prolonged period. The total number of column volumes was 10,000. Minimal change in column performance was seen after the test to Preparative Scale ApplicationsKromasil Columns are offered in a wide range of dimensions to enable simple, reproducible scale-up.

7 The highly robust nature of the silica and the availability of a wide range of particle sizes makes Kromasil an ideal choice for preparative following example (Figure 4) demonstrates the reproducible Chromatography obtained when scaling up from a column to a (2 ) 3. k values for butylbenzene plotted against column volumes of low pH eluent*Column: 250 x : CH3CN - H2O (70:30)Flow rate: velocity: 4. Scale-up reproducibility on Kromasil 10-C8 columnsColumn: 250 x 10mmFlow rate: and linear velocity as aboveColumn: 250 x 20mmFlow rate: and linear velocity as aboveColumn: 250 x rate: and linear velocity as aboveUracilBenzamideMethyl benzoateToluenePropylbenzeneButylbenzene 048121620 MinsRetention factor, k (butylbenzene)Column volumes of purge solvent, TFA in water, 70 C*The comparative data presented here may not be representative for all Columns Tel.

8 0118 930 3660143 hichrom LimitedKromasilOrdering Information - Kromasil Microbore ( ) ColumnsKromasil PhaseColumn Dimensions1 (mm)Guard Cartridges2 (For Columns )50 x x x x mPOAPOAPOAPOAPOA (5/pk)3 Silica (100 )MH3 SII05MH3 SII10MH3 SII15MH3 SII25MH3 SIIGCC4MH3 CSI05MH3 CSI10MH3 CSI15MH3 CSI25MH3 CSIGCC8MH3 CMI05MH3 CMI10MH3 CMI15MH3 CMI25MH3 CMIGCC18MH3 CLI05MH3 CLI10MH3 CLI15MH3 CLI25MH3 CLIGCNH2MH3 NHI05MH3 NHI10MH3 NHI15MH3 NHI25MH3 NHIGC5 mPOAPOAPOAPOAPOA (5/pk)3 Silica (60 )S05 SII05S05 SII10S05 SII15S05 SII25S05 SIIGCS ilica (100 )M05 SII05M05 SII10M05 SII15M05 SII25M05 SIIGCC1M05C1I05M05C1I10M05C1I15M05C1I25M 05C1 IGCC4M05 CSI05M05 CSI10M05 CSI15M05 CSI25M05 CSIGCC8M05 CMI05M05 CMI10M05 CMI15M05 CMI25M05 CMIGCC18M05 CLI05M05 CLI10M05 CLI15M05 CLI25M05 CLIGCNH2M05 NHI05M05 NHI10M05 NHI15M05 NHI25M05 NHIGCCNS05 CNI05S05 CNI10S05 CNI15S05 CNI25S05 CNIGCDiolS05 DII05S05 DII10S05 DII15S05 DII25S05 DIIGCP henylM05 PHI05M05 PHI10M05 PHI15M05 PHI25M05 PHIGCM icrobore ( )

9 ColumnsMedium Bore ( ) ColumnsKromasil PhaseColumn Dimensions1 (mm)Guard Cartridges2 (For Columns )50 x x x x m 276 290 304 332 120 (5/pk)3 Silica (100 )MH3 SID05MH3 SID10MH3 SID15MH3 SID25MH3 SIDGCC4MH3 CSD05MH3 CSD10MH3 CSD15MH3 CSD25MH3 CSDGCC8MH3 CMD05MH3 CMD10MH3 CMD15MH3 CMD25MH3 CMDGCC18MH3 CLD05MH3 CLD10MH3 CLD15MH3 CLD25MH3 CLDGCNH2MH3 NHD05MH3 NHD10MH3 NHD15MH3 NHD25MH3 NHDGC5 m 259 267 282 327 120 (5/pk)3 Silica (60 )S05 SID05S05 SID10S05 SID15S05 SID25S05 SIDGCS ilica (100 )M05 SID05M05 SID10M05 SID15M05 SID25M05 SIDGCC1M05C1D05M05C1D10M05C1D15M05C1D25M 05C1 DGCC4M05 CSD05M05 CSD10M05 CSD15M05 CSD25M05 CSDGCC8M05 CMD05M05 CMD10M05 CMD15M05 CMD25M05 CMDGCC18M05 CLD05M05 CLD10M05 CLD15M05 CLD25M05 CLDGCNH2M05 NHD05M05 NHD10M05 NHD15M05 NHD25M05 NHDGCCNS05 CND05S05 CND10S05 CND15S05 CND25S05 CNDGCDiolS05 DID05S05 DID10S05 DID15S05 DID25S05 DIDGCP henylM05 PHD05M05 PHD10M05 PHD15M05 PHD25M05 PHDGCK romasil PhaseColumn Dimensions1 (mm)Guard Cartridges2 (For Columns )

10 50 x x x x m 276 290 304 332 120 (5/pk)3 Silica (100 )MH3 SIC05MH3 SIC10MH3 SIC15MH3 SIC25MH3 SICGCC4MH3 CSC05MH3 CSC10MH3 CSC15MH3 CSC25MH3 CSCGCC8MH3 CMC05MH3 CMC10MH3 CMC15MH3 CMC25MH3 CMCGCC18MH3 CLC05MH3 CLC10MH3 CLC15MH3 CLC25MH3 CLCGCNH2MH3 NHC05MH3 NHC10MH3 NHC15MH3 NHC25MH3 NHCGC5 m 259 267 282 327 120 (5/pk)3 Silica (60 )S05 SIC05S05 SIC10S05 SIC15S05 SIC25S05 SICGCS ilica (100 )M05 SIC05M05 SIC10M05 SIC15M05 SIC25M05 SICGCC1M05C1C05M05C1C10M05C1C15M05C1C25M 05C1 CGCC4M05 CSC05M05 CSC10M05 CSC15M05 CSC25M05 CSCGCC8M05 CMC05M05 CMC10M05 CMC15M05 CMC25M05 CMCGCC18M05 CLC05M05 CLC10M05 CLC15M05 CLC25M05 CLCGCNH2M05 NHC05M05 NHC10M05 NHC15M05 NHC25M05 NHCGCCNS05 CNC05S05 CNC10S05 CNC15S05 CNC25S05 CNCGCDiolS05 DIC05S05 DIC10S05 DIC15S05 DIC25S05 DICGCP henylM05 PHC05M05 PHC10M05 PHC15M05 PHC25M05 PHCGC1 Other column dimensions available please enquire 2 Use with free-standing