Description of the Mississippi INBRE Imaging Facility ...

1 Description of the Mississippi INBRE Imaging Facility Equipment SpectraMax M3 Microplate Reader The M3 microplate reader provides dual monochromatic optics allowing for a wide range of applications from UV-visible absorbance to HTRF. Fluorescence intensity is also standard with spectral scanning across the wavelength range in 1nm increments and up to 4 wavelength pairs per protocol supporting FRET based assays. With SoftMax Pro Data Acquisition & Analysis Software. NanoDrop ND-1000 The NanoDrop ND-1000 UV/Vis Spectrophotometer allows microquantitation of only 1ml of sample with reproducibility. This allows researchers to measure samples up to 50-fold higher in concentration than can be measured on classical 1 cm curette-based systems, virtually eliminating the need for dilutions.

2 The various modes of use include: Nucleic Acids, Microarray, Protein A280, Proteins and labels, Protein BCA, Protein Bradford Protein Lowry, Cell Cultures and general UV-Vis measurement. CFX 96 Real-time PCR Detection system The CFX96 system includes two components: Optical reaction module and C1000 thermal cycler base. The experiment setup window provides quick access to the files and settings needed to set up, edit and run an experiment. The qx200 droplet digital PCR system The qx200 droplet digital PCR (ddPCR ) system provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications. The qx200 droplet digital PCR system consists of two instruments, the qx200 droplet generator and the qx200 droplet reader, and their associated consumables to reduce bias from amplification efficiency and PCR inhibitors.

3 BioRuptors Pico Sonication The Bioruptor Pico is the latest innovation in shearing and represents a new breakthrough as an all-in-one shearing system optimized for shearing micro-volumes of 5 l to larger volumes of up to 2 ml. This wide range enables a large variety of applications such as DNA shearing for Next-Generation Sequencing library preparation, RNA shearing, chromatin shearing, protein extraction from tissues and cells, and FFPE DNA extraction. Shearing is a critical step in Next-Generation Sequencing library preparation, chromatin immunoprecipitation, and ChIP-seq. SPD 111-115 Evaporator & Concentrator Speed evaporator and concentrator for standard evaporation and lyophilize your samples.

4 The universal vacuum system with Oil-Free vacuum pump and -50C refrigerated vapor trap. ChemiDoc MP The ChemiDoc MP system is a full-feature instrument for gel or western blot Imaging . It is designed to address multiplex fluorescent western blotting, chemiluminescence detection, and general gel documentation applications. Its features are based on CCD high-resolution, high-sensitivity detection technology, and modular options to accommodate a wide range of samples and support multiple detection methods. The system is controlled by Image Lab software to optimize performance for fast, integrated, and automated image capture and analysis of various samples. NGC Quest 10 Plus chromatography system The NGC Quest 10 Plus chromatography system is designed to suit smaller labs with basic purification needs.

5 The NGC Quest 10 Plus instrument has automated 10 ml/min pumps that provide accurate gradients for high-resolution separations for any application. The NGC Quest 10 Plus system has a multi-wavelength detector with simultaneous four-wavelength monitoring for high-accuracy detection of proteins, peptides, and nucleic acids combined with conductivity measurements. Fractionated samples can be easily collected from analytical- to preparative-scale purifications using the BioFrac fraction collector. Tissue-Tek Cyro3 Microtome/Cryostat Tissue-Tek Cryostat provides superior sections from 1 to 99 m in 1 m increments using a temperature-controlled blade holder along with 360 specimen orientation.

6 A color-touch LCD screen provides a visual display of critical information such as current chamber temperature, section thickness, and trimming thickness. LSM 510 UV/META The Zeiss 510 META combines an expanded laser system with an advance META detector for better multi-fluorescence Imaging and separation of flurochromes which could not be imaged together on other instruments. The META detector also allows better separation of background auto fluorescence from fluorescent dye emission. The Zeiss META has 6 lasers (lines) of excitation (405, 458, 488, 514, 543, and 633nm). The 405 laser allows for Imaging DAPI and other blue-emission fluorochromes. Bioanalyzer 2100 The Agilent 2100 Bioanalyzer improves DNA, RNA and Protein analysis through accurate and reproducible sizing and quantitation, automated and reproducible quantitation, rapid visualization of sample quality, high sensitivity with only a small amount of sample, significant time savings-10 to 12 samples in 30 minutes, easy comparison or sharing of sample data, simple, robust protocols and reagents, and reduced use and waste of hazardous chemicals.

7 Typhoon FLA7000 by GEThe Typhoon FLA7000 by GE is a nonconfocal variable mode laser scanner offering high speed and resolution for precise quantitation of proteins, nucleic acids, and sensitive and quantitative measurements of radioisotopic labels. Using predefined methods, the system can image a sample measuring 24 x 40 cm in minutes, offering high throughput for the multiuser lab environment. The system provides several Imaging modes: fluorescence and chemifluorescence, filmless autoradiography, and digitization of stained samples ( Coomassie blue and silver stain). Leica DM IL LED Fluo The Leica DM IL LED Fluo SE is the new inverted microscope and high-performance instrument for use in any field that requires fluorescence techniques for living specimens.

8 It is also available with the new LED illumination without heat development and high stability, plenty of space for operation, large working distances. The fluorescence slider holds three filter blocks and each filter block comprises an optimally matched combination of excitation, reflection. Leica M165 FC Stereomicroscope The Stereomicroscopes combine a large zoom range and high-resolution performance in a single system to enable a broad range of research tasks with just one microscope. Leica M165 FC features encoding of the focus, zoom, filters, iris diaphragm and transmits the information to the Leica LAS software. The microscope configuration and optical data is available on a computer at any time.

9 Nikon Fluorescence ECLIPSE 80i The Nikon Eclipse 80i is built for optimal digital Imaging , ergonomics, and versatility of digital research microscopy. Concentrating on stability and mechanical precision, the Eclipse 80i incorporates wide spectrum violet corrected (VC) plan Apo optics, and offers the widest range of research configurations obtainable. Also to capture images with excellent resolution and uniform brightness up to the edges. Innova 44 Incubator Shakers The Innova 44 Stackable Incubator Shakers are large-capacity orbital shakers that utilize an eccentric counter-balanced drive mechanism. They provide horizontal plane rotary motion in either a 1-inch or 2-inch diameter circular orbit and temperature control from 4oC to 80oC.

10 Freezone Triad Freeze Dryer The FreeZone Triad Freeze Dry Systems offer it all for lyophilization and stoppering tray dryer and sample freeze drying with four sample valves on the left side. Samples for both types of freeze drying can be run at once. The chamber pre-freezes samples to save money and time, eliminating the need for a separate freezer and product transfer. Avanti J26S XPI centrifuge The Avanti J-26S series is the only high performance centrifuge system . Built upon benchmark performance, including high speed, low heat output, imbalance tolerance, and low noise. The Avanti J-26S is sustainable, easy-to-use, versatile, and safe. Optima MAX-XP The Optima MAX-XP Ultracentrifuge is a power-packed solution that delivers fast, efficient separations from samples as small as 175 l up to ml, and at speeds up to 150,000 RPM and more than 1,000,000 x g.