包涵体纯化蛋白复性操作流程 - detaibio.com

1 Tel: 025 ActiveProteinSolutions DNA 2M 50mMTris 1mMEDTA TritonX-100 NaCl 50% SDS Sarkosyl DTT 8-10M 70-90%

2 95% Tel: 025 ActiveProteinSolutions 6-8M 4M 2M 4M 1. Tel: 025 ActiveProteinSolutions2. CD 3.

3 IEX RP-HPLC CE 4. 5. 6. ELISA WESTERN 1. PEG6000-20000 2. PDI PPI PDI PPI 3. HSP 2 3 t-PA 5. 5%-30% 6. SEC HIC IEC IMAC Tel: 025 ActiveProteinSolutions Tiss-Tearor?

4 Spectra/Por?6 SDS-PAGE A 1000mL 1mol/LTris-HCl 50mL 50 50mmol/L 50mL 5% A+50% DOC N- SKL N- 1. 18mL A 2mL20%DOC 10min 2. 4 C 13000r/min 10min C #1 #2 4 C 13000r/min 10min 3. #1 A 20%SKL 30min 4.#1 4 C 13000r/min 10min Tel: 025 ActiveProteinSolutions 1. A+ 1mg/mL 2. A 10 SKL C 200mL 2000mL A 8h 4h 150uL HPLC 7. 4 C 8000r/min 20min 8. J POROS50S 9. A 5mL A+1mol/LNaCl A 10.

5 4mL/min 11. A 15min 60min 4mL/min 0-1mol/LNaCl/ A 260nm 280nm 4mL A+50% Tel: 025 ActiveProteinSolutions