Transcription of Detection and Confirmation Salmonella TT & SC - …
1 BioM rieux Canada, Henri-Bourassa Boulevard WestSt. Laurent (Qu bec) Canada H4S 1P7 Tel: (1) 514 336 7321 Fax: (1) 514 807 0015bioM rieux, Anglum Road Hazelwood, MO 63042 : (800) 634 7656 Fax: (800) 657 further details, consult the product package insert. 2009 bioM rieux, inc. bioM rieux, the blue logo, APi, chroMiD, ViDAS AnD ViteK Are regiStereD trADeMArKS belonging to bioM rieux SA or one of itS SubSi DiArieS. PrinteD in SLMD etection and Confirmation of Salmonella (TT & SC)Protocol validated by AoAc international (official Method of Analysis, Method no. ) VIDAS SLMT8034 Tetrathionate Broth Base20 x 10 mlT7079 Selenite Cystine Broth20 x 10 mlT8080M Broth20 x 10 ml30702 VIDAS SLM60 testsM1016 Hektoen Enteric Agar10 platesM1031 XLD10 plates7113 Bismuth Sulfite Agar500g43621 ChromID Salmonella20 plates21341 VITEK 2 GN20 cards20100api 20E25 strips32700rapid ID 32 E25 stripsTest < SalmonellaTest within 45 min* or Heat at 95-100 C / 15 min2.
2 Confirmation18 2 hrs / 42 C 1 mlStreak TT, SC and M broths to Hektoen, XLD and Bismuth Sulfite. Incubate plates and confirm suspect colonies10 ml Tetrathionate (TT)api System For manual identificationVITEK 2 Compact For automated identification42 C / 6 hrs10 ml M-broth42 C / 6 hrs10 ml M-broth1 ml 18 2 hrs / 35-37 C 18 2 hrs / 35-37 C Continue Incubation18 2 hrs / 35-37 C Continue Incubation225 ml non-selective medium (as described in BAM)1 ml1 mlSerological confirmationIdentification 24 2 hrs / 35 C 10 ml Selenite Cystine (SC)1 ml1 ml25 g or 25 ml1. Detection using VIDAS SLMRaw meats500 lStore 2-8 CStore 2-8 CStore 2-8 CStore 2-8 CVIDAS SLMHeat & Go / 15 1 min*
