一分子解析技術に基づく 次世代DNAシーケンサーの開発

1 DNA 2013 4 25 1 FIRST DNA 2013 1 Quantum Biosystems Inc. CEO CTO Scientific Advisory Board FIRST *1 1 Panasonic DNA DNA *2 DNA

2 *1 FIRST 1 *2 SCIENTIFIC REPORTS | 2 : 501 2012 July 2 *1 2011 $ 3 Billion 2016 $ billion *2 $1,000 Roche Illumina $ billion TOB ( BGI( Complete Genomics $117 million Illumina/Oxford Nanopore Technologies, Life Technologies/Genia 1101001,00010,000100,0001,000,00010,000, 000100,000.))

3 000200220042006200820102012 Moore s Law 2 sequencer 1 sequencer *1 23 and me, Ancestry, Navigenics *2 BCC research 2012; DNA Sequencing: Emerging Technologies and Applications $/ 3 1 (~2005) 2 (2005~2011) 3 (2011~) ABI 3130 ABI 3500 ABI 3730(XL) Roche 454 Illumina GA Illumina HiSeq 2000 AB(Life Technologies) SOLiD / Sequencing by synthesis/ligasion Sanger 1 pH Pac Bio RS Ion torrent PGM Oxford Nanopore Technologies 4 3~4 4 Brown Univ.

4 Boston/ Harvard Univ. Life Technologies Illumina 3 (2011~) 4 ? Quantum sequencing NHGRI IBM, Samsung, Intel, Roche 2005 *1 *2 (2012 7 ) *1 FIRST 1 *2 SCIENTIFIC REPORTS | 2 : 501 2012 July NHGRI( ) 2 5 John Thompson: Simplified Sample Preparation for Single-Molecule Sequencing: Single-Step Capture and Sequencing of Natural DNA Steve Gordon.

5 Low Cost, Long Read Sequencing by Synthesis Steven Benner: Reagent Innovations to Enable Unlimited, Low Noise Multiplexing, Parallel Genome Analysis, and Genomics Applications John Nelson: Closed Complex DNA Sequencing Jekwan Ryu: Deep-Submicron Optical Detection for High-Density High- Throughput DNA Sequencing Stuart Lindsay: Sequential Reads of DNA Sequence by Recognition Tunneling Tomoji Kawai: Development of Gating Nanopores for Single-Molecule DNA Electrical Sequencing Marija Drndic: Graphene-Based Nanopore Devices for DNA Sequencing Eric Ervin: Towards Direct Sequencing of Single Stranded DNA Using a Biological Nanopore Ping Xie: Nanowire-Nanopore Transistor for DNA Detection Steven Gordon: Low-Cost, Flexible Mini Sequence for Medical Diagnostics Josh Ryu: Deep-Submicron Optical Detection for High-Density High-Throughput DNA Sequencing Kalim Mir: Single-Molecule (Nano)Genomics: from Chromosome Structure to DNA Sequence Xiaohua Huang: Direct Real-Time Single-Molecule DNA Sequencing Fangyuan Ding: Single-Molecule Mechanical Identification and Sequencing Tomoji Kawai.

6 Single Molecule Electrical Sequencing of DNA and RNA using Integrated Gating Nanopores Geoffrey Barrall: Direct Nanopore Sequencing of Single Stranded DNA John Kasianowicz:TBA Hiral Patel: A Very-Large-Scale Integration (VLSI) Approach To Rapid Single-Molecule Sequencing by Tunneling Ken Shepard: Integrated Nanopore Sensing Platform with Sub-Microsecond Temporal Resolution Rashid Bashir: 3-D Stacked Graphene-Dielectric Nanopore Architectures for Sensitive Detection of DNA and DNA Protein Complexes Larry Scipioni: High-Throughput, Reproducible Machining of Solid State Nanopores Sadig Faris: Is Error-Free Sequencing Possible, Affordable and Fast?

7 2011 NHGRI Advanced DNA Sequencing Technology Development Meeting Agenda National Human Genome Research Institute funded by National Institutes of Health Department of Health and Human Services 2012 NHGRI Advanced DNA Sequencing Technology Development Meeting Agenda 6 7 (Honkura, Toshihiko) CEO 1999 2001 2001 2002 2003 2005 2009 MBA 2011 1996 1998 2001 2001 2002 2007 2008 2011 2007 2011 (Taniguchi.)

8 Masateru) CTO Scientific Advisory Board 8 (Kawai, Tomoji) 1974 1976 1983 1992 2001 2002 2004 2007 2009 Baba, Yoshinobu) 1986 1986 1990 1997 2004 2004 2005 2006 2007 2008