Dr.Maisch GmbH VisionHT™ Ultra High-Pressure Columns See ...

1 GmbH VisionHT Ultra High-Pressure Columns See Separations with Speed and Clarity Ultra -fast separations with superior ef ciency, sensitivity and resolution Exceptional stability for long column life times Comprehensive sub2 m stationary phase offering 12,000psig pressurerating compatible with all ultrahigh-pressure LC systems hplc Columns | small molecule VisionHT Columns offer a new level of performance in HPLC. The powerful combination of high-strength m media with Ultra -low volume hardware, delivers new clarity to your separations and maintains exceptional column lifetime.

2 Complex samples resolve 95% faster with 4x greater sensitivity when compared to traditional x 150mm, 5 m Columns . And with a wide variety of phases available, the possibilities are endless. Increase Speed by 95% and Gain 4x Better Sensitivity 1. Thiourea 2500 mAU 2. Toluene 2. 2000 3. Propylbenzene 2500 mAU 4. Butylbenzene Impurities 2 1500. 2000 1 3 not seen on 1000 traditional 1500 Column: VisionHT C18, m, 4 5 m Columns . 13 500. 1000. 4 Flow Rate: 0. 500. 0 1 2 Min. 2000 0. 1500 0 2 4 6 8 10 12 14 Min. System: Agilent1200.

3 Column: Traditional C18,5 m, Mobile Phase: (45:55)20mM Potassium Phosphate, 1000 :Acetonitrile 1 2 Flow Rate: Detector: UV at 254nm 500 3 4 Temperature: Ambient 0. 0 2 4 6 8 10 12 14 Min. E ciency and Retention Times Remain Constant after Exposure to 12,000psig and 2000 Injections 5 2. Pyridine Column Stability Comparison, 50 x 2mm 3. Phenol 4. Methyl Alanine 300 VisionHT Column 1 VisionHT Column 5 2+3 5. 4-ButylBenzoicAcid VisionHT Column 2 VisionHT Column 6. VisionHT Column 3 VisionHT Column 7 1 6. Toluene 250. Plates/Meter (000).

4 VisionHT Column 4 Competitor Sub 2 m Column InITIAL. 4 6. 200. 150 0 2 4 6 8 Min. 100. Competitor 5. 50 Sub 2 m Column 2+3. 7713. 9427. 10284. 11141. 11998. 12855. 13712. 14569. 15426. 16283. 17140. 0. 857. 1714. 2571. 3428. 4285. 5142. 6000. 6856. 8570. 1. 6 FInAL. Column Volumes 4 (After 2000 injections). 0 2 4 6 8 Min. Column: Mobile Phase:50mMKH 2PO4, :CH3CN(60:40). Flow Rate: High-Pressure competitor sub 2 m column lost e ciency at routine Before and after chromatograms show a constant level of 12,000psig pressures.

5 VisionHT Columns remain stable. performance after 2000 injections. VisionHT Ultra High-Pressure Columns Comprehensive High Purity m Selectivity Options Sub 2 m particles deliver e ciency and speed, but critical to success is having the right stationary phase selectivity. Six VisionHT high purity phases are available, each with unique separation beneits. C18-HL, with maximum bonded phase coverage, is ideal for complex hydrophobic samples. Use C18-B. hplc Columns | small molecule for basic compounds at neutral pH, often a requirement for mass spec work in the pharmaceutical industry.

6 Reserve the C18 and C18-P for fastest analysis times. Both offer increased polar interactions to make neutral, non-polar compounds elute faster and retain polar compounds longer. The HILIC and Silica packings are normal phases that typically use near exclusive organic mobile phases ; an advantage when seeking highest mass spec sensitivity. VisionHT phases have exceptionally rigid silica structure that withstands routine use of 12,000psig pressure often required in this new technology. Reproducible silica synthesis, bonding and column packing guarantee low bleed and excellent column-to-column consistency.

7 VisionHT Phase Speciications Base Particle Carbon Pore Surface pH. Packing Material Size Load Size Area Endcapped Range* Feature Beneits 2. C18-HL Spherical m 10% 120 220m /g Yes 1 10 Fully bonded silica, ultraHigh capacity for hydrophobic compounds. Good Silica high purity silica peak symmetry. C18-B Spherical m 5% 120 220m2/g Proprietary 1 10 High purity silica and Improved performance of basic compounds at Silica unique endcapping. neutral pH. Better sensitivity and peak shape by mass spec for basic compounds, without the need for acidiied mobile phases .

8 C18 Spherical m 6% 100 200m2/g Yes 1 10 Moderate silica exposure Classic reversed-phase selectivity. Reduced Silica bonding is optimized for speed and sensitivity. 2. C18-P Spherical m 5% 100 200m /g No 1 10 High silica exposure, low Unique polar selectivity. Low carbon load gives Silica carbon load fastest reversed-phase elution times while retaining polar compounds longer. HILIC Spherical m NA 120 220m2/g No 2 8 Polar phase with shorter Peak reversal compared to reversed-phase. Ideal Silica equilibration times. for very polar compounds with high organic mobile Shipped in ACN/Water.

9 phases for improved sensitivity by MS. Silica Spherical m NA 120 220m2/g No 2 8 Traditional normal phase For isomeric separation of non-aqueous compatible Silica for use in 100% organic compounds by absorption chromatography. mobile phases *Choiceof bufferiscriticalatpH>8. VisionHT Selectivity VisionHT Reversed-Phase Selectivity Separate Highly Basic Components at 10 k' Phenol neutral pH with Vision HT C18-B. k' Methyl Alanine 9. k' Butyl Benzoic Acid 8 3 1. Phenylephrine k' Toluene 2. Diphenhydramine 7 2. 3. Amitriptyline 6.

10 K' 5 1. 4. 3. 2. 1. 0 0 1 2 3 4 5 Min. C18-P C18 C18-B C18-HL Column: VisionHT C18-B, m, Mobile Phase:50mM Ammonium Formate pH7:Methanol(20:80). Column Temp: 40 C. Flow Rate: Detector: UV at 210nm VisionHT Ultra High-Pressure Columns Unique Column Hardware Advantages VisionHT Hardware Speciications Dead volume: <15nL. You can erode the beneits of sub 2 m media if packed in Wetted materials: 316 Stainless Steel, PTFE. an ine cient column design. VisionHT hardware uses Port Geometry: 10 32, for Industry Standard &. thin screens, instead of traditional frits, to retain media and Waters connection minimize dead volume.