Empower PDA Software - Waters Corporation

1 Empower PDA SoftwareGetting started Guide34 Maple StreetMilford, MA 0175771500031503, Revision ANOTICEThe information in this document is subject to change without notice and should not be construed as a commitment by Waters Corporation . Waters Corporation assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, the use of this document. 2002 Waters Corporation . PRINTED IN THE UNITED STATES OF AMERICA. ALL RIGHTS RESERVED.

2 THIS DOCUMENT OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THE and Waters are registered trademarks, and Empower is a trademark of Waters , Windows, and Windows NT are registered trademarks of Microsoft other trademarks or registered trademarks are the sole property of their respective of 10 Chapter 1 PDA Software Overview .. What Is PDA Software ? .. Tutorial Restoring the PDA Project .. 18 Chapter 2 Viewing PDA Data .. Tutorial Viewing Data in Displaying the 3D Zooming In on Plots .. Extracting a Chromatogram .. Extracting a Annotating Chromatograms and Spectra.

3 Adding Erasing Annotation Tools .. 46 Chapter 3 Peak Purity Processing .. Tutorial Deriving Chromatograms .. 48 Table of ContentsTable of Developing a PDA Processing Method .. Viewing, Modifying and Saving the Method Viewing the Peak Purity Calculation .. Reviewing Peak Purity Results .. Using Spectrum Index .. Using the Results Window .. 75 Chapter 4 Library Matching .. Tutorial Steps in Creating a Library .. Steps in Library Matching .. Creating a New Library .. Matching Spectra to a Library .. Modifying an Existing Processing Method for Library Matching .. Creating a New Processing Method for Library Matching.

4 Performing Library Reviewing Library Matching Results .. 109 Table of Contents5 Chapter 5 Printing Reports .. Previewing a Background 117 Appendix A Default PDA Reports .. 120 Index .. 140 List of Figures61-1 Empower Login Dialog 181-2 Empower Pro Window .. 191-3 Configuration Manager .. 201-4 Restore Project Wizard - Start Software Page .. 211-5 Configuration Manager with Restored PDA_Default Project .. 222-1 Empower Pro Window .. 242-2 Browse Project Dialog Box .. 242-3 Project 252-4 Review with PDA Data, no Chromatogram Extracted .. 262-5 Maximized Review Main Window in 3D Layout .. 272-6 Review Toolbar Buttons.

5 282-7 Sample 3D Plot Window .. 302-8 Creating the Zoom Box .. 312-9 Zoomed View of the Contour 322-10 Scaling Tab .. 332-11 Extracted Chromatogram at Wavelength 254 nm .. 342-12 Extracted Chromatogram at Wavelength 280 nm .. 352-13 Overlaid Chromatograms at Wavelengths 254 and 280 nm .. 362-14 Extracted Spectrum at 372-15 Overlaid Extracted Spectra .. 382-16 Spectra Table .. 392-17 Normalized Spectra .. 402-18 Viewing Spectrum Points: Absorbances Versus 412-19 Annotation Tools .. 422-20 Annotation Object Properties Dialog Box for an Object .. 432-21 Annotation Object Properties Dialog Box for Text.

6 442-22 Annotation 45 List of FiguresList of Figures73-1 Project Window of the PDA_Default Project .. 493-2 Review Main Window with a Max Plot Chromatogram .. 503-3 Review Main Window with a Chromatogram at 254 nm .. 513-4 Processing Method Wizard Dialog Box .. 523-5 New Processing Method Dialog 523-6 Integration - Peak Detection 1 Page, Full 533-7 Setting the Peak Width 543-8 Integration - Peak Detection 2 Page .. 553-9 Setting the Threshold Parameter .. 563-10 Integration - Integration Region Page .. 573-11 Integration - Peak Rejection Page .. 583-12 Names and Retention Times Page .. 593-13 PDA Purity/Matching Page.

7 603-14 PDA Spectral Contrast 613-15 Setting the Noise 623-16 Processing Method Name Page .. 633-17 Integrated Chromatogram in Review .. 643-18 Method Set Editor Window .. 653-19 Method Set Tree Pane .. 663-20 Save Current Method Set Dialog Box .. 663-21 Method Set Editor Window after Modifying and 673-22 Purity Angle and Purity Threshold Values in the Peaks Table .. 683-23 Apex Spectrum .. 703-24 Apex Spectra Overlaid with Maximum Impurity 713-25 Maximum Impurity Spectrum with Peak 1 Spectra Zoomed .. 723-26 Maximum Impurity Spectrum with Peak 2 Spectra Zoomed .. 733-27 Maximum Impurity Spectrum with Peak 3 Spectra Zoomed.

8 743-28 Results Window .. 753-29 Purity Plot .. 763-30 Plot Properties Dialog 773-31 Purity Plot with Maximum Impurity 78 List of Figures84-1 Steps in Library Matching .. 814-2 Selecting Data for a Library .. 824-3 Review Main 834-4 Open an Existing Method Set Dialog 844-5 Paraben Stds Chromatogram .. 854-6 Create a New Library Dialog Box .. 864-7 Spectrum Review with Spectra Selected .. 874-8 Add Spectrum to Library Dialog 884-9 Project Window of the PDA_Default Project .. 894-10 Review Main 904-11 Integrated 254 nm Chromatogram of Mixture .. 914-12 Processing Method Window .. 924-13 PDA Library Search Tab.

9 934-14 Processing Method Wizard Dialog Box .. 944-15 New Processing Method Dialog 954-16 Integration - Peak Detection 1 Page, Full 964-17 Setting the Peak Width 974-18 Integration - Peak Detection 2 Page .. 974-19 Setting the Threshold Parameter .. 984-20 Integration - Integration Region Page .. 994-21 Integration - Peak Rejection Page .. 1004-22 Names and Retention Times Page .. 1014-23 PDA Purity/Matching Page .. 1024-24 PDA Spectral Contrast 1034-25 Setting the Noise 1044-26 PDA Match Library Page .. 1054-27 Processing Method Name Page .. 1064-28 Processed Chromatogram with Library Match 1074-29 Integrated Chromatogram with Library Match Results.

10 1084-30 Spectrum Index with Library 1094-31 Overlaid Library Match Spectra .. 1104-32 Results Window Displaying Library Match Plot .. 111 List of Figures94-33 Library Match Plot and Library Match Tab .. 1124-34 Triple Plot: Library Match .. 1135-1 Project 1145-2 Open Report Method Dialog 1155-3 Preview of an Example Report .. 1165-4 Project 1175-5 Background Processing and Reporting Dialog 1185-6 Sample 11910 PrefaceThe Empower PDA Software getting started guide describes the basics of how to use the Empower PDA option to develop a PDA processing method for peak purity and library matching and to review PDA guide is intended for a wide variety of users whose familiarity with computers and Software ranges from novice to guide contains the following.