Enzymes for biocatalysis - Novozymes

1 BiocatalysisBiocatalysis involves the implementation of natural catalysts, such as Enzymes , in place of chemical catalysts in synthetic process. Compared to chemical catalysts, Enzymes offer: higher reaction rates milder reaction conditions high reaction specificity with no side productsThis change can enable new, more sustainable routes for the production of intermediates and active pharmaceutical ingredients (APIs). biocatalysis has become an increasingly important tool for medical, process and polymer chemists, allowing the development of efficient and highly attractive synthetic process on an industrial scale. Use of Enzymes in catalysis is a well-established technology within the chemical industry.

2 An advantage of Enzymes in organic synthesis is their remarkable selective properties, which provide commercial benefits including: high selectivity in production of single stereoisomers fewer side reactions less reprocessing and purification steps easier product separation less pollutionThe combination of all of these advantages leads to a reduction in catalysts work by lowering the activation energy (Ea) for a reaction, thus dramatically increasing the rate of the reaction. As a result, products are formed faster and reactions reach their equilibrium state more rapidly. Most enzyme reaction rates are millions of times faster than those of comparable uncatalyzed reactions.

3 As with all catalysts, Enzymes are not consumed by the reactions they catalyze, nor do they alter the equilibrium of these reactions. However, Enzymes do differ from most other catalysts in that they are highly specific for their smarter chemical synthesisEnzymes for Novozymes Novozymes is the world leader in biological solutions. Together with customers, partners and the global community, we improve industrial performance while preserving the planet s resources and helping to build better lives. As the world s largest provider of enzyme and microbial technologies, our bioinnovation enables higher agricultural yields, low-temperature washing, energy-efficient production, renewable fuel and many other benefits that we rely on today and in the future.

4 We call it Rethink 2016 No. 2015-09752-03 Novozymes does not promote or support the use of Enzymes as Active Pharmaceutical Ingredients (APIs) or excipients. Novozymes Enzymes are industrial bulk products and are not produced according to pharmaceutical standards. If Enzymes are used as process aids, it is the responsibility of the API or excipient manufacturer to assure that the Enzymes are suitable for the intended use, including but not limited to evaluation of purity and absence of carry over in the final product. Laws, regulations, and/or third party rights may prevent customers from importing, using, processing, and/or reselling the products described herein in a given manner.

5 Without separate, written agreement between the customer and Novozymes to such effect, this document does not constitute a representation or warranty of any kind and is subject to change without further notice.+45 44460044(DK)+1 9194943331(US)+91 2025604401(IN)+86 13817879003 (CN) Novozymes A/S Krogshoejvej 36 DK 2880 Bagsvaerd nameEC number TypeSourceFormOptimum usage conditionsActivityApplicationsNovozym specific LipaseAspergillus nigerImmobi-lized30 60 C, pH 5 9 10000 PLU/gStereoselective hydrolysis of esters, transes-terification and dynamic kinetic resolution of alcoholsLipozyme CalB specific LipaseAspergillus nigerLiquid30 60 C, pH 5 95 KLU/gStereoselective hydrolysis of esters, transes-terification and resolution of alcoholsNovocor AD specific LipaseAspergillus oryzae Liquid30 60 C, pH 5 96 KLU/gStereoselective hydrolysis of hin-dered esters of alcoholsNovozym.

6 3 LipaseAspergillus oryzae Immobi-lized30 50 C, pH 7 10275 IUN/gStereoselective hydrolysis of esters and trans esterificationPalatase 20000 , 3 LipaseAspergillus oryzae Liquid30 50 C, pH 7 1020000 LU-MM/gStereoselective hydrolysis of esters Stereo-selective hydrolysis of estersLipozyme TL , 3 Lipase Aspergillus oryzae Immobi-lized50 75 C, pH 6 8250 IUN/gStereoselective hydrolysis of esters and trans esterificationLipozyme TL 100 , 3 Lipase Aspergillus oryzae Liquid20 50 C, pH 7 10100 KLU/gStereoselective hydrolysis of esters and dicarboxylatesNovozym 51032 oryzae Liquid35 70 C, pH 7 1015 KLU/gSteroselective hydrolysis of esterswResinase oryzae Liquidup to 90 C50 KLU/gHydrolyzes esterbonds in glyceridesLecitase oryzae Liquid35 60 C10 KLU/gLipase that hydrolyzes esterbonds in glyceridesAlcalase L endo-peptidase bacillus licheniformisLiquid30 65 C, pH 7 AU-A/gStereoselective hydrolysis of amino esters and selective estersAlcalase L, endo-peptidase bacillus licheniformisLiquid30 65 C, pH 7 AU-A/gStereoselective hydrolysis of amino esters and selective estersAlcalase endo-peptidase bacillus licheniformisLiquid30 65 C.

7 PH 7 AU-A/gStereoselective hydrolysis of amino esters and selective estersSavinase 12 endo-peptidase bacillus licheniformisGranulate30 70 C, pH 8 1012 KN-PU-S/gStereoselective hydrolysis of amino esters and selective estersSavinase 16 endo-peptidase bacillus licheniformisLiquid30 70 C, pH 8 1016 KN-PU-S/gStereoselective hydrolysis of amino esters and selective estersEsperase endo-peptidase bacillus licheniformisLiquid30 70 C, pH 8 108 KNPU-E/gSerine endoprotease that hydrolyzes inter-nal peptide bondsNeutrase amylolique faciensLiquid40 50 C, pH AU-N/gMetallo endoprotease that hydrolyz-es internal peptide bondsrTrypsin proteaseFusarium venenatumGranulatepH USP/mgHydrolysis of amid end ester bonds of lysine and arginine at carboxyl terminalCatazyme 25 nigerLiquid25 40 C, pH 4 625000 CIU/gDismutates hydorgen peroxide into dioxygen and waterToruzyme dextrin glucantransferaseBacillus licheniformisLiquid80 95 C, pH 5 KNU-CP/gCyclomaltodextrin glucanotrans-ferase that forms cyclomaltodextrin of various sizes from starch and similar substratesBAN 480 amyloliquefaciensLiquid55 75 C,pH 5 7480 KNU-B/gEndo- amylase that hydrolyzes ( )

8 -alpha-D-glucosidic linkages in starch polysaccharidesNovozym oryzeaLiquid 1000 LA-MU/gOxidizes various phenols, anilines, benzen-ethiols, metal ion complexes, and other compounds into quinones or other oxidized compounds, with concomitant reduction of dioxygen to water