Excellent performance gives you complete ... - hplc.sk

1 Excellent performance gives you complete confidence in your results Many column dimensions and bulk media, so a solution is never far away Easy scale-up, from analysis to purification and prep to processHPLCHPLC210 VARIAN, INC .Contents212 Application Guide215 USP Methods218 Pursuit XRs and Pursuit Columns234 Polaris Columns239 ChromSep Column Hardware241 PLRP-S Columns250 VariTide RPC Columns250 PL-SAX/PL-WAX Columns252 PL-SCX/PL-WCX Columns253 Hi-Plex Columns260 Prep to Process Solutions262 Dynamax Column Hardware263 High Efficiency Purification265 PLRP-S Columns and Media268 PL-SAX and PL-SCX Columns and Media270 SepTech Media271 FlowTrap Columns272 Load & Lock Prep Systems274 hplc Instrument Supplies283 LC Fraction Collector and DetectorsHPLCHPLC from Varian

2 Offers a very wide range of column types and packing materials. By controlling the manufacturing process from start to finish, you benefit from guaranteed, reproducible columns and packing purification of recombinant pharmaceuticals from bacteria helps elucidate their role in the transfer of drug , INC .HPLCA pplication and Phase Selection GuideDue to the fact that no application is the same or carried out in the same way, a standard choice of phase and/or hplc column may not lead to the required performance . Varian strongly believes that the best chromatographic performance is a delicate balance that calls for fine tuning of all the elements that participate in the hplc system.

3 This means that finding the best chromatographic phase and column dimensions for your application is a serious task. Varian s technical staff will help you in finding the right solution for your application, as well as providing advice on your operating system. Our aim is to deliver a total customer solution that creates the highest value possible for tables in this section will help you make the first phase selection based on the characteristics of your Columns and MediaSmall MoleculesAnalyte specific analysisCompound ClassSeparation MechanismSorbentLipidsSilver IonChromSpher LipidsPolycyclic Aromatic HydrocarbonsPursuit PAHO rganic AcidsLigand InteractionHi-Plex HIon Pair Reverse PhasePLRP-S 100 Monosaccharides and DisaccharidesLigand InteractionHi-Plex Ca (Duo)

4 Hi-Plex PbNormal Phase AminoPolaris NH2 Ion SuppressionPLRP-S 100 OligosaccharidesLigand InteractionHi-Plex CaHi-Plex NaSugar AlcoholsLigand InteractionHi-Plex CaHi-Plex Ca USPHi-Plex HHi-Plex KHi-Plex PbNormal PhaseNormal Phase AminoPolaris NH2 PolarReverse Phase C18 Pursuit XRs C18 Basic, PolarReverse Phase C18 Pursuit C18 Ion SuppressionPLRP-S 100 Large MoleculesOrganic solubleCompound ClassSeparation MechanismSorbentSynthetic - PolydisperseGel Permeation ChromatographyPLgel MIXED-APLgel MIXED-BPLgel MIXED-CPLgel MIXED-DPLgel MIXED-ESynthetic Limited MW RangeGel Permeation ChromatographyPLgel 50 PLgel 100 PLgel 500 PLgel 103 PLgel 104 PLgel 105 PLgel 106 OligomersReverse PhasePLRP-S 100 PLRP-S 300 Small MoleculesWater solubleCompound ClassSeparation MechanismSorbentPolarReverse Phase C18 Polaris C18-AH-bondingReverse Phase C18

5 Polaris C18-AmidePolaris C18-EtherPositional IsomersReverse Phase C8 Pursuit XRs C8 Pursuit C8 PolarReverse Phase C8 Polaris C8-AH-bondingReverse Phase C8 Polaris C8-EtherAromaticReverse Phase - DiphenylPursuit XRs DPStructurally SimilarReverse Phase - DiphenylPursuit DPExtremely PolarReverse Phase - OtherPursuit PFPE xtreme ConditionsReverse Phase - PolymericPLRP-S 100 PLRP-S 300 Organic solubleCompound ClassSeparation MechanismSorbentNon-polarNormal Phase SiPursuit XRs SiPolaris SiPolarNormal Phase AminoPolaris NH2 For more information, please visit or visit to place an Columns and MediaLarge MoleculesWater soluble continuedCompound ClassSeparation MechanismSorbentRecombinant Peptides and ProteinsReverse PhasePLRP-S 100 PLRP-S 300 PLRP-S 1000 PLRP-S 4000 Anion ExchangePL-SAX 1000 PL-SAX 4000 Cation ExchangePL-SCX 1000 PL-SCX 4000 Macromolecular PlasmidsReverse PhasePLRP-S 4000 Anion ExchangePL-SAX 4000 Purification of Small Molecules and PharmaceuticalsHigh performance , high resolution - shown with relevant instrumentationSeparation MechanismSorbentParticle Size ( m)

6 PrepStar SD-1/940-LCPrepStar SD-2 SepTech SkidHigh PerformanceHigh ThroughputHigh YieldNormal Phase SiPursuit XRs510 Normal Phase AminoPolaris NH2510 Reverse Phase - C18 Pursuit XRs C18510 Pursuit C18510 Polaris C18-A510 Polaris C18-Amide510 Polaris C18-Ether510 SepTech ST60 C1810 10 Reverse Phase - C8 Pursuit XRs C851010 Pursuit C8510 Polaris C8-A510 Polaris C8-Ether510 Reverse Phase - DiphenylPursuit XRs DP510 Pursuit DP510 Reverse Phase - PolymericPLRP-S 100 5, 81010-15, 15-20, 50 Large MoleculesWater solubleCompound ClassSeparation MechanismSorbentSynthetic - PolydisperseSize Exclusion ChromatographyPL aquagel-OH MIXEDS ynthetic Limited MW RangeSize Exclusion ChromatographyPL aquagel-OH 20PL aquagel-OH 30PL aquagel-OH 40PL aquagel-OH 50PL aquagel-OH 60 Synthetic PeptidesReverse PhaseVariTide RPCPLRP-S 100 PLRP-S 300 Synthetic Oligonucleotides DNA/RNAIon Pair Reverse PhasePLRP-S 100 PLRP-S 300 PLRP-S 1000 PLRP-S 4000 Anion ExchangePL-SAX 1000 Protected/DeprotectedPLRP-S 300 Polymers Compositional AnalysisPLRP-S 1000 PLRP-S

7 4000 For more information, please visit or visit to place an , INC .HPLCHPLC Columns and MediaPurification of Larger BiomoleculesSynthetic peptides - shown with relevant instrumentationSeparation MechanismSorbentParticle Size ( m)PrepStar SD-1/940-LCPrepStar SD-2 SepTech SkidHigh PerformanceHigh ThroughputHigh YieldReverse PhaseVariTide RPC6 SepTech ST150 10-C18101010 PLRP-S 100 81010-15, 15-20, 50 PLRP-S 300 81010-15, 15-20, 50 Synthetic oligonucleotides - shown with relevant instrumentationSeparation MechanismSorbentParticle Size ( m)PrepStar SD-1/940-LCPrepStar SD-2 SepTech SkidHigh PerformanceHigh ThroughputHigh YieldIon Pair Reverse PhasePLRP-S 100 81010-15, 15-20, 50 PLRP-S 300 81010-15, 15-20, 50 PLRP-S 1000 81030, 50 PLRP-S 4000 81030 Anion ExchangePL-SAX 1000 81030 Trityl-on/Trityl-offPLRP-S 100 505050 PLRP-S 300 505050 Recombinant peptides and proteins - shown with relevant instrumentationSeparation MechanismSorbentParticle Size ( m)

8 PrepStar SD-1/940-LCPrepStar SD-2 SepTech SkidHigh PerformanceHigh ThroughputHigh YieldReverse PhasePLRP-S 100 81010-15, 15-20, 50 PLRP-S 300 81010-15, 15-20, 50 PLRP-S 1000 81030 PLRP-S 4000 81030 Anion ExchangePL-SAX 1000 81030PL-SAX 4000 81030 Cation ExchangePL-SCX 1000 81030PL-SAX 4000 81030 Macromolecular plasmids - shown with relevant instrumentationSeparation MechanismSorbentParticle Size ( m)PrepStar SD-1/940-LCPrepStar SD-2 SepTech SkidHigh PerformanceHigh ThroughputHigh YieldReverse PhasePLRP-S 4000 810, 30 Anion ExchangePL-SAX 4000 810, 30As a global leader in preparative and process chromatography, Varian Inc.

9 Provides both silica-based and polymeric, high performance media to meet the needs of discovery, research and production. Options for linear scale-up from g/mg level through to multi kg cGMP production scale are more information, please visit or visit to place an hplc Columns Recommended for USPM ethodsThe following chart is a guide to selecting Varian hplc columns that meet the specifications set by the most categories several different types of columns are listed which differ in particle size, particle shape, and carbon load or surface area. In these cases, the USP column specifications are quite broad and several column types meet the basic specifications.

10 For example, an L1 specification calls for a column containing silica or ceramic packing material, 5 m to 10 m in diameter, bonded with octadecyl (C18) silane. Many commercially available columns meet the basic specifications. However, not all C18 columns are alike, and only a limited number of the available C18 columns from any vendor will actually perform the desired increased efficiency and resolution, we recommend spherical particles of 3 to 5 m diameter. Packings with higher surface areas typically provide increased offers a variety of columns for every category that is representative of the wide range of selectivities for each bonded phase.