食品中の FAME、脂肪酸、 - chem-agilent.com

1 FAME .. agilent J&W GC . 2.. (Fatty Acid Methyl Ester: FAME) . GC ( ) .. agilent J&W GC FAME .. ( ) . ( . ).. 3 . 3 6 . 3.. agilent J&W GC QC .. DB-FATWAX 3 6 / . / FAME FAME / . C4-C16. ( ) . 5 . DB-FastFAME FAME 8 . / / . / FAME .. 8 . CP-Sil 88 HP-88 / FAME . FAME AOAC AOCS Ce 1j-07 . CLA FAME (PHVO) . 9 . Select FAME / FAME . FAME CP-Sil 88 for CP-Sil 88 for FAME/HP-88 . FAME/HP-88 . GC/MS . ( 200 m). 10 . CP-Tap CB Chromspher . GC LC .. FAME . 12 . 4. DB-FATWAX : / FAME . DB-FATWAX (FAME) . (FAEE) FAME ? FAME (ECL) EPA DPA DHA . FAME FAME DB-FATWAX UI 3 4 % .. J. Dairy Science, WAX 48, 1582-1584, 1965.. : GC : agilent 7890B. pA : DB-FATWAX UI 30 m x mm m 160. 250 C 1 DB-FATWAX UI (p/n G3903-63008). 140. : 250 C = 25:1. 6 7 8. 120 5 9. 4 : 80 C 40 cm/s 100. 3. 80 : 80 C (1 ) 10 C/min 200 C.

2 2. 250 C. 60. FID: 40. 20. 1 : L. pA 2 4 6 8 10 min 160 1.. 140 250 C 50 DB-FATWAX UI 2.. 120 6 7 8 9 3.. 4 5. 100 4.. 80 3 5.. 2. 60. 6.. 7.. 40. 1. 20. 8. 4- . 2 4 6 8 10 min 9.. 250 C DB-FATWAX . (C1-C6 (C1-C6). : GC : agilent 7890B. : DB-FATWAX UI 30 m x mm m (p/n G3903-63008). : 250 C = 50:1 40 cm/s : 38 cm/s pA . 180 1 : 10 C/min 100 C 250 C 260 C (10 ). 160 FID: 280 C. 140. 8 11 12 13 14 : 1 L. 10 15. : mg/mL. 120 6 7 9. 4. 100 5. 80 3 16. 60 2. 40. 1. 9.. 20. 2. 10.. 2 4 6 8 10 12 14 16 18 3. 11.. 4. 12.. 250 C 1 DB-FATWAX 5. 13.. FID 6. 14.. 7. 15.. 8. 4- 16.. 5. FAME . A. FAME . C24:0/C22:6n3. C24:1.. FAME C22:6n3 . C24:1 Rs > C10:0. C24:0. C6:0. C8:0. C12:0. 42 43 44. C22:6n3. C24:1. C4:0. B. C24:0. C22:6n3. C24:1. C11:0. C18:1 +C18:1 . C14:0. C16:0. C13:0. 48 49 50.)

3 C14:1. C22:0. C18:0. C18:2 . C15:0. C20:0. C20:3 n6. C22:1n9. C15:1. C20:4n6. C20:5n3. C16:1. C22:2n6. C18:2 . C20:2 n6. C18:3 n6. C20:1n9. C18:3 n3. C20:3n3. C23:0. C21:0. C24:0. C17:0. C17:1. C24:1. C22:6. 5 10 15 20 25 30 35 40 45 . DB-FATWAX DHA . : GC : agilent 7890B : 50 C (2 ) 50 C/min . : DB-FATWAX UI 30 m x mm 174 C (14 ) 2 C/min 215 C. m (p/n G3903-63008) (25 ). : 250 C / FID: 280 C :40 mL/min :400 mL/min . 50:1 :25 mL/min : 40 cm/s 50 C : 1 L. 6. 2 (PolyUnsaturated Fatty Acid: PUFA) .. 3 6 FAME . PUFA No 2 ( FAME). C18:1n9. C18:0. C16:0. C18:2n6 (LA). : C20:4n6 (ARA). GC : agilent 7890B. : DB-FATWAX UI 30 m x mm m (p/n G3903-63008). : 250 C / . C18:3n3 (ALA). C20:5n3 (EPA). C22:6n3 (DHA). 100:1. C22:5n3 (DPA). : mL/ . C18:3n6. C22:4n6. C18:1n7. C16:1n7. : 140 C 15 C/min 190 C (11 ).

4 C20:3n6. C20:2n6. C20:1n9. 4 C/min 220 C (20 ). C14:0. FID: 280 C :40 mL/min : 400 mL/min . :25 mL/min : 1 L. 5 10 15 20 25 30 35. : PUFA ( ). EPA DHA . 3/6 FAME . PUFA No 3 ( FAME). : 2 GC : agilent 7890B. 3 8 17. 1 : DB-FATWAX UI 30 m x mm . m (p/n G3903-63008). : 250 C / . 100:1. : 30 cm/s 180 C. 7 9. 19 : 180 C (2 ) 2 C/min 210 C (35 ). 5 13. 4 FID: 280 C :40 mL/min : 14 16 400 mL/min :25 mL/min 6 10 12. 11 15 18 : 1 L. : PUFA ( ). 5 10 15 20 25 30 35 40 45 min EPA DHA . 7. agilent J&W DB-FastFAME: / / FAME . DB-FastFAME HP-88 CP-Sil 88 FAME . DB-FastFAME . FAME / FAME . FAME 8 C18:1 C18:2 . FAME (DPA EPA ) . C18:1 . C20:0. C18:2 . C18:1 . C18:2 . C18:3 n 6. C18:3 n 3. C20:1n9. DB-FastFAME FAME . C20:4n6. C20:3n6. C20:3n3. C21:0. Rs = C18:1 . Rs = C20:0. C18:2 . Rs = C18:1.

5 Rs = C18:2 5 6. C18:3 n 6. C18:3 n 3. C20:1n9. C20:4n6. C20:3n6. C20:3n3. Rs = C21:0. 5 6. C16:0. C10:0. C12:0. C14:0. C8:0. C18:1 . C18:2 C18:1 . C6:0. C18:0. C13:0. C11:0. C14:1. C15:0. C15:1. C4:0. C20:0. C18:2 . C16:1. C18:3 n 6. C22:0. C18:3 n 3. C17:1. C16:0. C24:0. C20:3n3. C20:1n9. C20:3n6. C20:4n6. C21:0. C20:2. C10:0. C12:0. C17:0. C22:2. C23:0. C22:1. C14:0. C20:5. C24:1. C22:6. C8:0. C18:1 . C18:2 C18:1 . C6:0. 1 2 3 4 5 6 7. C18:0. C13:0. C11:0. FAME 8 AOCS AOAC . C14:1. C15:0. C15:1. C4:0. 5991-8706EN: 37 . C20:0. C18:2 . C16:1. C18:3 n 6. C22:0. C18:3 n 3. C17:1. C24:0. C20:3n3. C20:1n9. C20:3n6. C20:4n6. C21:0. C20:2. C17:0. C22:2. C23:0. C22:1. C20:5. : C24:1. C22:6. GC : agilent 7890B. : DB-FastFAME 20 m x mm m (p/n G3903-63010). 1 2 3 4 5 6 7. : 250 C / 50:1.

6 : 28 psi : 80 C ( ) 65 C/min 175 C 10 C/min . 185 C ( ) 7 C/min 230 C . FID: 260 C :40 mL/min :400 mL/min . :25 mL/min : 1 uL. : 37 FAME . 8. agilent J&W CP-Sil 88 for FAME HP-88:FAME . FAME . CP-Sil 88 for FAME HP-88 C6-C26 / FAME . / (PHVO) FAME. AOCS AOAC (AOAC AOCS Ce 1j-07 ) .. 5 C18:1 . Norm. 90 : 80 GC : agilent 6890. : HP-88 100 m x mm m (p/n 112-88A7). 70 : 250 C / 50:1 . (p/n 5183-4647). 60. : 2 mL/min 50 : 120 C (1 ) 10 C/min 175 C (10 ) . 5 C/min 210 C (5 ) 5 C/min 230 C . 40. (5 ). 30 FID: 280 C. : 1 uL. 20. 10. 15 16 17 18 19 20. agilent HP-88 16 FAME 50 .. (CLA) C18:2 FAME . CLA . (t8 c10-CLA ). C18:2 9c, 11t C18:2 8t, 10c C18:2 11c, 13t C18:2 10t, 12c C18:2 . : GC : agilent 6890. : CP Sil 88 for FAME 100 m x mm m C18:2 9t, 11c/10c, 12t C18:2 9t, 11c/10c, 12t (p/n CP7489).

7 : 260 C . : 30 psi C18:2 . : 170 C. 260 C. C18:2 7c,9t FID: : L. : TBME FAME 2 %. : Dr. Dahlke, Hamburger Fettchemie 37 43 min Brinckman & Mergell, GmbH. CLA . 9. Select FAME:FAME CP-Sil 88 for FAME/HP-88 . Select FAME CP-Sil 88 for FAME HP-88 GC / . / FAME ( C18 ) . 275 C 290 C 50 C . FAME GC/MS C18:1 200 m Select FAME 3 FAME . / FAME C18:1 . C18:1 . C18:0. C18:1n9c C18:1n12c C18:1.. C18:1n11t C18:1n10t : C18:1 . C18:1n11c GC : agilent 7890B. : Select FAME 200 m x mm (p/n CP7421). C18:1n9t C18:1n12t C18:1n15c : 250 C 1:20. C18:1n10c C18:1n8t C18:1n7t C18:1n13c : 520 kPa C18:1n14c : 185 C. FID: 250 C. : L. 46 54 min / FAME . / . : GC : agilent 7890B. 3 4 5 7 9 11. : GC . : Select FAME 50 m x mm m (p/n CP7419). : 1:100 T = 250 C. : 130 kPa ( bar 19 psi). 1 : 185 C. FID: 250 C.

8 : 1 L. 2. : ( ). 6 8. 10 20 1. C16:0 8. C16:1 9 15. C18:1 14 . 12 15 2. C8:0 9. C18:0 16. C18:1 15 . 16. 13. 17 3. C10:0 10. C18:1 17. C18:2 9 12 . 14 18 4. C12:0 11. C18:1 9 18. C18:2 9 12 . 19 5. C14:0 12. C18:1 11 19. C18:2 9 12 . 6. C14:1 13. C18:1 12 20. C18:2 9 12 . 7. C16:0 14. C18:1 13 . 0 17 min 17 20 / Select FAME 1 . FAME . 10. FAME . FAME .. ISO NIST ERM (CoA) .. FAME mix 36 component mix 1 mL FAME . AOAC . ISO-16958 I IDF 238 . FID . FAME mix C4-C24 even carbon saturated .. components 1 mL FAME ( . ) . FAME . C16-C18 . FAME mix in rapeseed oil 1 mL .. QC . C4-C24 FAME : GC : agilent Intuvo 9000. : DB-FastFAME 20 m x mm m Intuvo . : 250 C / 150:1. Intuvo : 200 C. : 45 psi : 50 C ( ) 250 C/min 175 C 20 C/min 240 C (2 ). C10:0. C12:0. FID: 250 C :40 mL/min :400 mL/min :25 mL/min : C8:0.

9 1 L. C14:0. C16:0. C18:0. C6:0. C20:0. C22:0. C24:0. C4:0. 1 2 3 4 5 min Intuvo DB-FastFAME 20 m x mm x m (p/n G3909-63005) 5 C4-C24 FAME (p/n 5191-4278). 11. CP-TAP CB for Triglycerides/Chromspher Lipids: . GC CP-TAP CB for Triglycerides . CP-TAP CB for Triglycerides .. CP-TAP CB . 360 C . UltiMetal .. POP POO. PPP. PLO. : GC : agilent 7890B. : GC . POS. : CP-TAP CB for Triglycerides 25 m x mm m (p/n CP7483). OOO. : 340 C (1 ) 355 C (1 C/min). MOP PLS SOO. : H2 100 kPa (1 bar 15 psi). OLO. MPP PPS PLL : . SLO. MOO. MLP SOS AOO. : % L. MLO OLL. SOA. MOM PSS : FID. : L. 0 16 min : % . C46 C56 24 agilent J&W. CP-TAP CB for Triglycerides 16 . M : ( ) C14:0. P : ( ) C16:0. O : ( -9- ) C18:1. L : ( -9,12- ) C18:2. S : ( ) C18:0. A : ( ) C20:0. : GC : agilent 7890B. PPP. PPS : GC.

10 SOO. : CP-TAP CB for Triglycerides 25 m x mm . OOO. PSS SSO.. m (p/n CP7483). SSS. : 280 C (1 ) 355 C 3 C/min T. 54. T. 52. : H2 100 kPa (1 bar 15 psi). T. 50. T. 48. : . T. T T. 44 46. T. T. 40 42. T. 26 T28T30 T32. T. 34. T. 36 38. : % L. 0 29 min : FID. CP-TAP CB for Triglycerides 11 29 . 12. HPLC ChromSpher Lipids . ChromSpher Lipid Ag+ ? LC .. CP-TAB CB for Trigylceride .. CP-Sil 88 for FAME .. Nutrition Research, 44, 1-8, 2017.. : SSE- : HPLC. : ChromSpher Lipids 250 x mm . SSM- . : A: / 50/50 (v/v) B: . : t = 0 t = 3 100 % A T = 3 t = 45 100 %. SSS- . A 50 % A/50 % B. : mL/min : 25 C. : ACS. : 20 L. : g/mL. : . SMM- . SMD- . SEM- MMM- . S : . M : . D : 1. 0 30 min E : (18:1t). :Dr. Deffense, Fractionnement TIRTIAUX, Fleurus, Belgium . PPL. OPO (sn2 ). : : ChromSpher 5 Lipids 250 mm x mm (p/n 28313) x 2.