モリナガ FASPEK 牛乳ウエスタンブロットキット ラクトグロ …

1 - 1 - 2016 9 7 FASPEK - 2-1-16 236-0003 20 55mL 1 20 2- 40 55mL 1 20 50mL 1 10 g/mL 2- 4 500 L 1 - 1 1. 22 9 10 286 2. - 1. 2.

2 20 25 C 3. 4. 2- 5. 2- 1. 2. 3. - - - - / - IgG - - IgG FASPEK - - IgG - - 2.

3 IFN-700G [ ( )] Laemmli Sample Buffer [BIO-RAD] 1 2- Electrophoresis Power Supply - EPS 1001 [GE] 2 STC-808 [ ] SeeBlue Plus2 Pre-Stained Standard [Invitrogen] 3 Q-PAGE mini 12well [ ] Tris-BES 10x [ ] 400x [ ] SD [BIO-RAD] 1 Amersham Hybond PVDF m [GE] 2 [BIO-RAD] 1 10x Tris/Glycine [BIO-RAD] 1 10x TBS [BIO-RAD] 1 Tween-20 VECTASTAIN ABC-AP Rabbit IgG kit [VECTOR] 4 Alkaline Phosphatase Substrate Kit IV <BCIP/NBT> [VECTOR] 4 100 mM Tris/ pH 1 2 GE 3 4 VECTOR LABORATORIES, Inc.

4 1. Laemmli Sample Buffer 2- 19 1 2. 1:1:1:17 * 3. Tris-BES (10x) 10 (400x) 400 4. 10x Tris/Glycine 7 2 1 5. 10x TBS 10 Tween-20 * 6. * 7. - 100 8.

5 VECTASTAIN ABC-AP Rabbit IgG kit IgG 10,000 9. - VECTASTAIN ABC-AP Rabbit IgG kit A B 10 mL 1 * 30 10. Alkaline Phosphatase Substrate Kit IV <BCIP/NBT> 1 2 3 100 mM Tris/ pH 10 mL - 3 - I 1. (1) 1 g 50 mL 19 mL (2) 12 90 110 / 3 cm (3) pH pH pH (4) 3,000 g 20 ( ) (5) 1 2 5 2.

6 (1) 1 2 5 (2) 10 g/mL 1 g/mL g/mL (1) II 1. 2. 7 L 10 g/mL 1 g/mL g/mL 20 L * 3. 60 mA 1 cm III 1. 2 * 30 30 * * 2.

7 15 V 1 IV 1. 1 4 C 2. 1 3. 5 3 4. 30 5 3 5. - 20 5 3 6. 100 mM Tris/ pH 15 7. 3 10 8. 15 9. - MW= 18,400 - 4 - 0 g/g 10 g/g 8 (50 )

8 10 2 0 g/g 10 g/g 10 g/g 90 22 9 10 286 1. 2. 3. 1. 2 8 C 2. 1. 2. 3. 4.

9 FASPEK - 0 g/g 10 g/g 0/16 16/16 0/16 16/16 0/16 16/16 0/16 16/16 0/16 16/16 I II 60 mA/ III 15 V 1 IV 1 4 C 1 5 3 30 5 3 20 5 3 100 mM Tris/ pH 15 3 10 ( ) 15