Filters Fluorescence and Life Science Instrumentation

1 Filters for Fluorescence and Life Science Instrumentation Catalog 2009. Omega Optical celebrates 40 years! Omega Optical has been designing and producing optical Filters for Fluorescence microscopy and Instrumentation longer than any other optical filter company. We are grateful for the advancements in Science , technology and outer space made with our products. Any project, challenging or simple, we are happy to assist. Our guiding philosophy has always been to find solutions. Optical Filters come in many levels of performance, and are produced using various technologies. Omega Optical has 40 years of experience working with laboratories and instrument designers to achieve the required performance. Along with years of experience, we bring a corporate commitment to cooperatively explore, understand and ultimately refine the solution.

2 We have a strong staff of scientists from the biological field, as well as in chemistry and physics that is essential to gain the highest quality of remote analysis. While this catalog mainly represents Filters for Fluorescence applications, we also manufacture Filters for the following: * Medical Diagnostics: Point-of-Care Instrumentation , Hematology, Macular Degeneration, Real-Time Patient Monitoring and others. * Industrial: Environmental Monitoring, Machine Vision Systems, Spectroscopy, Lasers, Color Correction, Source Leveling and others. *D efense, Aerospace and Astronomy: Infra-Red Vision Systems, Targeting Systems, LIDAR , Conflict / Weapon Simulators, Outer Space Based Instrumentation , Ground-Based Telescope Systems, and others. The entire Omega Optical staff, from the Applications Engineering team, to the Sales team, to the Manufacturing teams are anxious to work with you.

3 Our aim is to assure that the most appropriate solution will be provided with the highest quality product. Pricing We value our relationship with you, our customer. We will strive to produce the best product at the most competitive price, and meet your critical need for performance, quality, and service. While pricing guidelines are reflected on our website, please contact us for best pricing. Warranty 3 Years All of the products listed are backed by a MINIMUM 3-year satisfaction warranty. Please note that liability does not include obvious misuse of the product and is limited to repair or replacement of the product purchased. Omega Optical accepts no liability for consequential or incidental damage caused by use of the product. How to find the right filter inside this catalog Stock and Standard Filters : These are arranged by fluorophore and emission wavelength on pages 12-18.

4 By filter set number: Please refer to Filter Set Reference Table on page 19. By center wavelength (CWL) and cut-on and cut-off: Select from a list of excitation and emission Filters , and dichroic beamsplitters on pages 20-21 and 3rd millennium 22-23. By fluorophore: Please see Fluorophore Reference Table on pages 24-26. By application: Fluorescent Proteins (pages 27-28), FRET (page 29), Qdot (pages 32-33), M-FISH (page 36), Ratio Imaging (page 37), Multi-band (page 38), Multi-color Imaging (pages 34-35), Real-time Imaging (page 37), Flow Cytometry (pages 43-45). For current product listings, specifications, and pricing: +44 020 8204 9517. 2. Introduction General Info .. 4. Technology .. 5. Filter Terminology .. 5. Filters For Microscopy .. 6. Filter Descriptions & Nomenclature.

5 7. Choosing the Optimal Filter or Filter Set .. 8. Typical Epi-fluoresence Configuration.. 9. Frequently Asked Questions and Answers .. 10-11. Products Stock Filters .. 12-13. QuantaMAX Filters and Sets .. 12. Quanta-Vivid (Q-Vivid) Filters and Sets .. 13. ALPHA Vivid Filters and Sets .. 13. Standard Filters .. 14-18. Filter Sets Reference Table .. 19. Excitation Filters by Center Wavelength .. 20. table of contents Dichroic Mirrors by Cut-On .. 20-21. Emission Filters by Center Wavelength .. 21. 3rd Millennium Filters by Cut-On and/or by Cut-Off .. 22-23. Fluorophore and Best Set Reference Table .. 24-26. Fluorescent Protein Sets .. 27-28. Fluorescent Protein Multi-band Sets .. 28. FRET Filters .. 29. Optimizing Filter Sets for FRET Applications.

6 30-31. Quantum Dot Filters and Filter Sets .. 32-33. Pinkel Sets .. 34-35. Sedat Sets .. 35. Photoswitchable Protein Set .. 35. M-FISH Filters .. 36. Ratio Imaging Filters .. 37. Multi-band Sets .. 38. Emission Color Chart for Multi-band Sets .. 39. Caged Compound Sets.. 40. Multi-Photon Filters .. 40. Beamsplitters & Mirrors .. 40. Neutral Density Filters .. 40. IR Blocking Filters .. 41. IR-DIC Filters .. 41. Polarizing Filters .. 41. Fluorescence Reference Slides .. 41. Microscope Cubes and Filter Holders .. 42. Flow Cytometry Filters .. 43-45. Appendix Light Sources and Detectors.. 46. Build-a-Filter .. 47. Curv-o-matic .. 47. l ca How to order .. 47. ti Op ga Ome General Information Filter Types and Technology QuantaMAX: Omega Optical's QuantaMAX Filters are manufactured using state-of-the-art Energetic Process coating technology for high durability, and low temperature sensitivity.

7 These high throughput Filters provide excellent results in all applications. They are especially effective in applications where photons are scarce and exposure times are critical. QuantaMAX filter designs consider not just peak filter transmission and fluorophore probability curves, but also optimized band placement in relation to light sources, and blocking in relation to detector sensitivity. ALPHA Technology: Omega Optical's proprietary coating process produces extremely steep slopes and precise edge location of cut-on and cut-off wavelengths. In addition, it provides the ability to place transmission and rejection regions exceptionally close together, and high attenuation between the passband and the rejection band. 3rd Millennium Technology: The most critical feature of filter design is the transition from blocking to transmission.

8 Precise location of band edges, coupled with steep slopes, allows for optimal transmission of spectral energy. 3rd Millennium Filters are specified by the critical cut-on and cut-off edges, resulting in much more accurate band placement and bandwidth, compared to industry standard Filters that are defined by a Center Wavelength (CWL) and a Bandwidth (FWHM). Multi-band Filters : Omega Optical is a co-holder of the patent on all multi-band Filters ; including dual-band, triple- band, and quad-band designs. Others: Bandpass Filters Full Mirrors Anti-Reflective Coatings Wideband Filters Edge Filters Neutral Density Filters Longpass Filters Multi-band Filters Dichroic Mirrors Rejection Band Filters Shortpass Filters Narrow-band Filters Beamsplitters Color Correction Filters Hot/Cold Mirrors Custom Filters OEM Production Quantities For optimum instrument system performance we recommend collaborative engineering for custom filter solutions.

9 Omega Optical is a leading supplier for Fluorescence based instruments and applications including: Microplate Readers, DNA. Sequencers & Analyzers; Gene Chip Readers; Flow Cytometers and Cell Sorters; Real Time PCR Analyzers; Scanners and Imaging Systems; High-Throughput and High-Content Systems; Fluorescence Microscopes, and others. Custom Filters Small Quantities Custom Filters in single or small quantities are available to ship in 5 10 days from our extensive inventory of components. Visit Build-a-Filter at Aftermarket Filters If you are in need of a filter that did not come with your original equipment, please go to our Build-A-Filter tool on our website. Select your instrument, tell us your filter wave length, or cut-on or cut-off, and we do the rest.

10 No need to specify physical dimensions. See page 47. For current product listings, specifications, and pricing: .. +44 020 8204 9517. 4. Omega Optical employs the use of 23 active coating chambers and has always utilized a wide variety of coating technologies for complex high-phase thickness thin films. We utilize Physical Vapor Deposition (PVD) with resistive element heating, Ion assisted e-Beam, and Dual Magnetron Reactive Ion Beam Sputtering; the world's most advanced sputtering technology. No single technology is ideal for every application. As a world-class manufacturer, Omega Optical knows the attributes and limitations of each and utilizes multiple technologies to achieve best results. In addition, we consider the following to be important considerations when recommending or designing the filter that's right for your instrument development: angle of incidence, polarization, substrate material, system speed, and temperature effects.