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Final TIPS and Tricks HPLC Troubleshooting
fittings. QUse a low-volume detector cell. QInject small sample volumes. Increasing Extra-Column Volume Extra-Column Dispersion Page 26 Peak Broadening Extra-Column Volume Column: StableBond SB-C18, 4.6 x 30 mm, 3.5 μm Mobile Phase: 85% H 2O with 0.1% TFA : 15% ACN Flow Rate: 1.0 mL/min Temperature: 35°C Sample: 1.
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