Fluorophore and BHQ Multiplexing ... - .NET Framework

1 These dye recommendations apply to black hole Quencher (BHQ) Probes of LGC, Biosearch Technologies and may not apply to other probe formats. For tips and guidelines related to qPCR and SNP genotyping, please visit: chart is intended to guide you through the dye selection process for your oligonucleotide. Fluorophore and quencher combinations may be designed to support applications such as probe-based qPCR, SNP genotyping, CNV, presence/absence and more. Multiplexing recommendations for BHQ Probes and primersFluorophore and BHQ selection chartIntegrated tools. Accelerated science.@LGCB iosearch | trademarks and registered trademarks mentioned herein are the property of their respective owners. All other trademarks and registered trademarks are the property of LGC and its subsidiaries. Specifications, terms and pricing are subject to change. Not all products are available in all countries.

2 Please consult your local sales representative for details. No part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording or any retrieval system, without the written permission of the copyright holder. LGC Limited, 2021. All rights reserved. GEN/579/C5/1121 Instrument*CompanyCalibrationrequired?Dy e 1 Dye 2 Dye 3 Dye 4 Dye 5 Dye 6 IntelliQube Biosearch TechnologiesYe sFAM CIV-550 TAMRAROX Quasar 670 Nexar Biosearch TechnologiesNoFAMHEXCAL Fluor Red 610 SNPline Biosearch TechnologiesNoFAM CIV-550 TAMRAQ uasar 670MX3000P Agilent NoFAM CIV-550 CAL Fluor Red 610 Quasar 670Mx3005P AgilentYe sFAM CIV-550 TAMRACAL Fluor Red 610 Quasar 670MX4000 AgilentNoFAM CIV-550 CAL Fluor Red 610 Quasar 670qTOWER3 SeriesAnalytik JenaYe sFAM CIV-550 TAMRACAL Fluor Red 610 Quasar 670 Quasar 705 PRISM 7000 Applied Biosystems Ye sFAM CIV-550 TAMRAROX7300 Applied BiosystemsYe sFAM CIV-550 TAMRAROX7500/7500 FastApplied BiosystemsYe sFAM CIV-550 TAMRAROXQ uasar 670 PRISM 7700 Applied BiosystemsYe sFAM CIV-550 TAMRAROX7900HT/7900HT FastApplied BiosystemsYe sFAM CIV-550 TAMRAROXQ uantStudio 3 SystemApplied BiosystemsYe sFAM CIV-550 TAMRACAL Fluor Red 610 QuantStudio 3D DigitalApplied BiosystemsYe sFAM CIV-550

3 CAL Fluor Red 610 QuantStudio 5 SystemApplied BiosystemsYe sFAM CIV-550 TAMRACAL Fluor Red 610 Quasar 670 QuantStudio 6 FlexApplied BiosystemsYe sFAM CIV-550 TAMRACAL Fluor Red 610 Quasar 670 QuantStudio 6 ProApplied BiosystemsYe sFAM CIV-550 TAMRACAL Fluor Red 610 Quasar 670 QuantStudio 7 FlexApplied BiosystemsYe sFAM CIV-550 TAMRACAL Fluor Red 610 Quasar 670 QuantStudio 7 ProApplied BiosystemsYe sFAM CIV-550 TAMRACAL Fluor Red 610 Quasar 670 QuantStudio 12k FlexApplied BiosystemsYe sFAM CIV-550 TAMRACAL Fluor Red 610 QuantStudio Absolute QApplied BiosystemsYe sFAM CIV-550 TAMRAROXQ uasar 670 StepOnePlus Applied BiosystemsYe sFAM CIV-550 TAMRAStepOne Applied BiosystemsYe sFAM CIV-550 ViiA 7 Applied BiosystemsYe sFAM CIV-550 ROXQ uasar 670 Quasar 705 MicBio Molecular SystemsNoFAMCAL Fluor Orange 560 CAL Fluor Red 610 Quasar 670 LineGene 9600 PlusBioer TechnologyYe sFAM CIV-550 CAL Fluor Red 610 Quasar 670 Quasar 705 LineGene K PlusBioer TechnologyYe sFAM CIV-550 CAL Fluor Red 610 Quasar 670 LineGene Mini SBioer TechnologyYe sFAM CIV-550 CAL Fluor Red 610 Quasar 670 QuantGene 9600 Bioer TechnologyNoFAM CIV-550 CAL Fluor Red 610 Quasar 670 Quasar 705 CFX Connect Bio-Rad LaboratoriesYe sFAM CIV-550 CFX Opus 96 Bio-Rad LaboratoriesYe sFAM CIV-550 CAL Fluor Red 610 Quasar 670 Quasar 705 CFX Opus 384 Bio-Rad LaboratoriesYe sFAM CIV-550 CAL Fluor Red 610 Quasar 670 CFX96 Touch Bio-Rad LaboratoriesYe sFAM CIV-550 CAL Fluor Red 610 Quasar 670 Quasar 705 CFX384 Touch Bio-Rad LaboratoriesYe sFAM CIV-550 CAL Fluor Red 610 Quasar 670iCycler iQ Bio-Rad LaboratoriesYe sFAM CIV-550 TAMRACAL Fluor Red 610 Quasar 670iQ 5 Bio-Rad LaboratoriesYe sFAM CIV-550 CAL Fluor Red 590 CAL Fluor Red 610 Quasar 670QX OneBio-Rad LaboratoriesYe sFAM CIV-550 Quasar 670 Quasar 705QX200 Droplet DigitalBio-Rad

4 LaboratoriesYe sFAM CIV-550 ContinuumDropworks (Bio-Rad Laboratories)Ye sFAM CIV-550 CAL Fluor Red 610 Quasar 670 FLUO star Omega Microplate ReaderBMG LabtechNoFAM CIV-550 SmartCycler Danaher Corp. (Cepheid)Ye sFAMCAL Fluor Orange 560 CAL Fluor Red 610 Quasar 670 SmartCycler IIDanaher Corp. (Cepheid)Ye sFAMCAL Fluor Orange 560 CAL Fluor Red 610 Quasar 670 Mastercycler ep RealplexEppendorfYe sFAM CIV-550 Biomark HDFluidigm Ye sFAM CIV-550 ROXEP1 FluidigmYe sFAM CIV-550 ROXEco Illumina Ye sFAM CIV-550 CAL Fluor Red 610 Quasar 670 MyGo MiniIT-IS Life Science sFAMCAL Fluor Gold 540 CAL Fluor Red 610 MyGo ProIT-IS Life Science Fluor Gold 540 CAL Fluor Red 590 CAL Fluor Red 610 Quasar 670 Quasar 705 QIAcuity Digital PCR SystemQiagenYe sFAM CIV-550 TAMRACAL Fluor Red 610 Quasar 670 Rotor-Gene Q 2plexQiagenNoFAM CIV-550 Rotor-Gene Q 5plexQiagenNoFAM CIV-550 CAL Fluor Red 610 Quasar 670 Quasar 705 RainDrop Plus RainDance TechnologiesNoFAM CIV-550cobas z 480 RocheYe sFAMCAL Fluor Orange 560 CAL Fluor Red 610 Quasar 705 LightCycler sFAMCAL Fluor Red 610 LightCycler 96 RocheYe sFAM CIV-550 CAL Fluor Red 610 Quasar 670 LightCycler 480 RocheYe sFAM CIV-550 CAL Fluor Red 610 Quasar 670

5 LightCycler 1536 RocheYe sFAM CIV-550 LightCycler NanoRocheYe sFAM CIV-550 CAL Fluor Red 610 Quasar 670 Naica dPCR System Prism3 Stilla Ye sFAM CIV-550 Quasar 670 Naica dPCR System Prism6 StillaYe sFAM CIV-550 TAMRACAL Fluor Red 610 Quasar 670 Quasar 705* Some instruments may require fluorescence calibration. Please visit for more dyesDYE-5 - T10 Recommended quencherBHQ dye quenching rangeExcitationEmissionBiosearch Blue 356454 BHQ-1 BHQ-0 430-520 nmFAM495520 BHQ-1 TET521536 BHQ-1 CAL Fluor Gold 540 VIC /TET/JOE522544 BHQ-1 CIV-550 VIC530550 BHQ-1 VICCIV-550/CAL Fluor Orange 560530550 JOE529555 BHQ-1 HEX535556 BHQ-1 BHQ-1 CAL Fluor Orange 560 VIC/HEX/JOE/CIV-550538559 BHQ-1480-580 nmQuasar 570Cy 3548566 BHQ-2Cy 3549566 TAMRA557583 BHQ-2 CAL Fluor Red 590 TAMRA569591 BHQ-2 BHQ-2*ROX 586610 BHQ-2 CAL Fluor Red 610 Texas Red/ROX/Alexa Fluor 594590610 BHQ-2Cy Red 597616LC Red 640619637 CAL Fluor Red 635LC Red 640618637 BHQ-2Cy 5646669 Quasar 670Cy 5647670 BHQ-2*, BHQ-3 Quasar 705Cy *, BHQ-3 BHQ-3 620-730 nmCy in BOLDFACE are available modifications for labeled dyes are also available in the form of nucleic acid chemistry reagents.

6 Please visit for current product Biosearch Technologies proprietary dyes* BHQ-2 dye is recommended for Quasar 670 and Quasar 705 fluorophores due to static quenching.