Transcription of For Research Use Only. Not For Use In Diagnostic Procedures.
1 Background: The prohibitins, called PHB1 and PHB2, are highly conserved proteins that are present in multiple compartments in eukaryotic cells. PHB1 is 30 kDa tumor suppressor protein involved in cell cycle control (1). PHB1 has been found in mitochondria, the nucleus and the plasma membrane, as well as extracellularly in circulation (2). In mitochondria prohibitins mainly exist as membrane-bound ring complexes and function as chaperones maintaining mitochondrial protein stability during protein synthesis and transportation (3,4). In the nucleus prohibitins interact with transcription factors such as Rb and p53 to regulate target gene transcription (2,5). Extracellular prohibitin can bind and activate C3 to enhance complement activation (6).
2 Specificity/Sensitivity: PHB1 Antibody detects endog-enous levels of total PHB1 : Polyclonal antibodies are produced by immunizing animals with a synthetic peptide correspond-ing to residues surrounding Ser252 of human PHB1. Antibodies are purified by protein A and peptide affinity References: (1) McClung, et al. (1995) Exp. Gerontol. 30, 99 124. (2) Mishra, S. et al. (2006) J. Cell. Mol. Med. 10, 353 363. (3) Nijtmans, et al. (2000) EMBO J. 19, 2444 2451. (4) Tatsuta, T. et al. (2005) Mol. Biol. Cell 16, 248 259. (5) Fusaro, G. et al. (2003) J. Biol. Chem. 278, 47853 47861. (6) Mishra, S. et al. (2007) Mol. Immunol. 44, 1907 1912.#2426 Store at 20 CPHB1 Antibodyrev. 12/22/15kDa Prohibitin 1PC12 COSN euro2A MCF-7 20014010080605040301020 Western blot analysis of extracts from MCF-7, Neuro2A, PC12 and COS cells, using PHB1 , IPEndogenousH, M, R, Mk32 kDaRabbit**Storage: Supplied in 10 mM sodium HEPES (pH ), 150 mM NaCl, 100 g/ml BSA and 50% glycerol.
3 Store at 20 C. Do not aliquot the antibody.*Species cross-reactivity is determined by western blot.** Anti-rabbit secondary antibodies must be used to detect this Antibody Dilutions: Western blotting 1:1000 Immunoprecipitation 1:100 For application specific protocols please see the web page for this product at visit for a complete listing of recommended companion Cross-Reactivity*Molecular 2010 Cell Signaling Technology, : For western blots, incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, Tween-20 at 4 C with gentle shaking, Research Use only . Not For Use In Diagnostic Cross-Reactivity Key:H humanM mouseR ratHm hamsterMk monkeyMi minkC chickenDm D.
4 MelanogasterX XenopusZ zebrafishB bovineDg dogPg pigSc S. cerevisiaeCe C. elegansHr HorseAll all species expectedSpecies enclosed in parentheses are predicted to react based on 100% Key:W WesternIP ImmunoprecipitationIHC ImmunohistochemistryChIP Chromatin ImmunoprecipitationIF ImmunofluorescenceF Flow cytometryE-P ELISA-Pepti
