水産ゲノム研究戦略 - fra.affrc.go.jp

1 22 3 I .. 1 II .. 2 .. 2 .. 4 .. LMO .. 6 .. 7 .. 8 .. 11 .. III .. 13 .. 13 .. 14 .. IV .. 21 .. 24 .. 24 .. 25 .. 27 .. 29 .. 31 1 I 21 21 12

2 21 7 8 12 2 II IMCB 21 Ensemble xml 2008 Jazz Arachne Ramen GenBank DDBJ EMBL 3 DNA 10 DNA 10 2 2006 8 6100 654 GenBank / DDBJ / EMBL BLAST DNA Clustal W DNA DNA

3 3 DNA LMO LMO 454 Tagami M.

4 Et al. 2008 BAC 2006) DNA 2009 2010 3 20 EU 20 Callorhinchus milii Danio rerio Tuebingen 4 Gasterosteus aculeatus Labeotropheus fuelleborni Domwe Island Latimeria menadoensis Indonesian Leucoraja erinacea Maylandia zebra Mazinzi Reef Mchenga conophoros Otter Point Melanochromis auratus Domwe Island Nothobranchius furzeri GRZ Nothobranchius furzeri MZM-0403 Nothobranchius kuhntae Oreochromis niloticus Oryzias

5 Latipes HNI Oryzias latipes Hd-rR Paralabidochromis chilotes Lake Victoria Petromyzon marinus Rhamphochromis esox Otter Point Takifugu rubripes Tetraodon nigroviridis 2003 2004 2007 2009 Draft 2009 2006 Draft) EST , SNP EST ( Suga et al. 2007, 2008 Hedgecock et al. 2005 Maldini et al. 2006 Ogden 2008 DNA mtDNA DNA 5 PCR mtDNA 16S-rRNA.

6 PCR-RFLP DNA 2006 mtDNA DNA cDNA cDNA Brinkman and Burnell 2009 Kawashima et al. 2003 Shiomi et al. 2004, Ota et al. 2006 Ueda et al. 2006 Ueda et al. 2008 Nagai et al. 2000 Nagai et al. 2002 EST Alexandrium Hackett et al.

7 2005, Erdner and Anderson 2006 Pseudo-nitzschia EST Dyhrman 2008 PCR 4 Pseudo-nitzschia SHI Alexandrium EST Kellmann et al. 2008 EST SHI FISH LAMP PCR DNA Nagai et al. 2009 EST Armbrust et al. 2004 6 fathead minnow Pimephales promelas cDNA Wintz et al. 2006 cDNA Soetaert et al. 2007 cDNA Watanabe et al.

8 2007 LMO Kapuscinski (2005) LMO LMO LMO LMO DNA mtDNA mtDNA DNA DNA

9 7 rDNA mtDNA rDNA mtDNA mtDNA rDNA DNA DOE GTL Ve n t e r et al. 2004 2006 CAMERA Sorcerer II, GOS Seshadri et al. 2007 2004 16 44 Marine Genome Europe MGE NITE JAMSTEC CAMERA 18 METAFUNCTIONS DNA 15 DeLong EF 2009, Christen 2008, Siezen and Wilson 2009 The Australian Genome Alliance 8 KORDI EU JAMSTEC 16.

10 18S-rRNA gyrA gyrB rpoB nirS nirK nosZ amoA pmoA nifH dsrA Gold Genome Online Database ( ; May 2009) 200 DNA RNA 35 167 5 1 EU Rohwer and Thurber 2009 1998 GH