기체크로마토그래피 Gas ... - contents.kocw.net

1 Gas Chromatography (GC) __ (GC)(GC)1GC __ (GC)(GC)2 MSD, AED ( ) __ (GC)(GC)3 Filters/TrapsData systemSyringe/SamplerInletsRegulators H RESET __ (GC)(GC)4 AirHydrogenGas Carrier Column gas system inlet column detector data systemInletsDetectors 1. A(FID) 2. B(TCD) 3. 4. __ (GC)(GC)54. 5. 6. 7.

2 ( )GC GC : (mobile phase) column (stationary phase) __ (GC)(GC)6 - ( ) .- (Phase) GC : . , , __ (GC)(GC)7 , . : : GC GLC (Gas -Liquid Chromatography): (solid support) __ (GC)(GC)8 GLC GSC.

3 GSC (Gas -Solid Chromatography): silica gel, (charcoal), (molecular sieve) (porous polymer) GSC vsGLC __ (GC)(GC)9 __ (GC)(GC)10 __ (GC)(GC)11GC , biologicalfluid __ (GC)(GC)12 biologicalfluid ( ) ( ) (Carrier Gas) (Carrier Gas) - - __ (GC)(GC)13- - , ( ) (CarrierGas)

4 - , , , (Carrier Gas) , , molecularsieve( ), ( ) __ (GC)(GC)14 (K) N (Carrier Gas) __ (GC)(GC)15 Flow rate Measurement __ (GC)(GC)16 : (column) -InjectionportTemp.: +20 C ( ) __ (GC)(GC)17: +20 C ( )-Injectiontype: .( )( chromatogram peak ,peak ).

5 (injectionport) ( ):Packedcolumn inlet [ 530mmcapillarycolumn;(megabore)packed ] ( / ):Capillarycolumn , __ (GC)(GC)18:Capillarycolumn , : : :Capillarycolumn column syringeneedle Install Pressure Temperature Program .. injectionport :10mL/min __ (GC)(GC)19 injectionport Cool-on injection inlet __ (GC)(GC)20 injectionport - (split)-> , __ (GC)(GC)21-> , ( )- (splitless): ( ) injectionport.

6 - - - 20 300oC __ (GC)(GC)22- 20 300oC __ (GC)(GC)23 Injection port and Detector (TCD, FID)