Transcription of GC 大量注入法を用いたカビ臭 ... - aisti.co.jp
1 18 2009 . GC 2-MIB . 1 2. 1 2 .. 2- 2-MIB . 16 4 g/L 10ppt) . 1/10 g/L 1ppt) . GC GC/MS GC/MS/MS .. GC LVI-S200 . GC/MS GC/MS/MS . GC/MS Jms-Q1000GC .. CombiPAL AMR . GC/MS/MS Quattro micro GC .. 7683B . C12H22O C11H20O. 2- 182 168. g/L g/L.. Structure & Physical Chemistry .. 100mL. 100mL . SAIKA-SPE PBX 20mg . SAIKA-SPE PBX 20mg) * 1 . *1 PBX . 2mL 2.. 5 . 2mL 2 . : 3:7) 1mL. 1% 200 10ppm d / 2 L. 1mL . 5 : 3:7) 1mL 100 . : GC/MS GC/MS/MS 25 L . (3:7)1mL 1% Scheme of sample preparetion (200) 10ppm . d / 2uL 1mL 100 . GC/MS GC/MS/MS 25uL.
2 High sensitive analysis of Musty Odor Substances (Geosmin and 2-MIB) in river water by GC/MS and GC/MS/MS using Large volume injection. Haruna Tanizawa(AISTI Science co.), Mari Sugihara(Jasco international co.). 18 2009 . (n=5) g/L( . 10 ) g/L( ) . GC/MS GC/MS/MS Table1, 2 . PTV Injector LVI-S200 (AiSTI Science) : Stomach Insert Injectoin Temp. 70 ( )-120 /min-240 -50 /min-260 (20min) . [ ] 1132-1129 RT=15:10. BP=112[299516] TIC=1369121 :10 SI=997. Auto Injector Combi PAL (AMR) : 50 L syringe 112. Injection Volume 25 L +05. GC Agilent 6890N.
3 Pre-column Deactivated silica capillary tube +05. 55. 69 83 97. Column MIGHTY Cap ENV-5MS 30m, m 125. 149. Column Oven Temp. 50 3min -10 /min-180 -25 /min-310 5min +00. m/z-> 80 120 160 200 240. Splitpurge 150mL/min( )-0( )-50mL/min(2min)-20mL/min MS JMS-K9 (JEOL). Detector Temp. 280 MIB. [ ] 647-658. RT=11:53. BP=95[224053] TIC=800215 :53 SI=994. MS Method SCAN : 50-250 +05 95. SIM : m/z=83,97,111,112,125,126,149 (Geosmin). m/z=95,107,108,135,150 (2-MIB). Condition of GC/MS +05. 107. PTV Injector LVI-S200 AiSTI Science Stomach Insert 55.
4 69 79. 121. 135. 150. Injector Temp. 70 ( )-120 /min-240 -50 /min-260 20min +00. m/z-> 80 120 160 200 240. Auto Samplor Agilent 7683B; 50 mL Syringe Injection Volume 25 L MS Spectrum GC Agilent 7890A. Column DB-5MS, 30m, df; Column Oven Temp. 50 3min -10 /min-180 -25 /min-310 5min . Splitpurge 100mL/min( )-0( )-50mL/min(2min)-20mL/min MS Quattro micro GC Waters . MS Method MRM 112 97 112 79 112 83 (Geosmin). 108 93 95 67 135 93 (2-MIB). Detector Temp. 280 . Multiplier 500V. Condition of GC/MS/MS.. Recovery and Reproducibility added to river water (Kinokawa in Wakayama) (%, n=5).
5 GC/MS GC/MS/MS. g/L compounds name Average RSD spike Average RSD spike Geosmin 2-MIB 2-Methylisoborneol *Sample were spiked at g/L( ) of Geosmin and 2-MIB. 90% RSD 10% .. BL F1:MRM of 3 channels,EI+ BL F2:MRM of 3 channels,EI+. 100% GC/MS 108 > 93. +003. 112 > 97. +004. 2-MIB. g/L GC/MS/MS % % Geosmin . 1 min 0 min SP 10ppt (1ppb) F1:MRM of 3 channels,EI+ SP 10ppt (1ppb) F2:MRM of 3 channels,EI+. 2-MIB 108 > 93 112 > 97. +003 Geosmin +004. 2-MIB % %.. 1 min 0 min STD 10ppt (1ppb) F1:MRM of 3 channels,EI+ STD 10ppt (1ppb) F2:MRM of 3 channels,EI+.
6 108 > 93 112 > 97.. +003 +004. 2-MIB Geosmin 500mL . % %. 100mL . 1. min 0. min GC/MS/MS Chromatograms of Geosmin and 2-MIB. (high: Blank in water, g/L spiked in river water, low:Standard g/L).
