Glucose Agar - HiMedia Labs

1 Please refer disclaimer AgarM1589 Glucose agar is used for determining the fermentation reactions of presumptive Enterobacteriaceae .Composition**IngredientsGms / pH ( at 25 C) **Formula adjusted, standardized to suit performance parametersDirectionsSuspend grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclavingat 15 lbs pressure (121 C) for 15 minutes. Mix well and pour into sterile Petri And InterpretationEnterobacteriaceae are widely distributed and found in soil, water, vegetation and the intestinal tract of animals. Examinationof foods, ingredients and raw materials, for the presence of marker groups such as coliforms or total Enterobacteriaceae , isone of the most common tests in food microbiology laboratory, because of the relative speed and ease with which the tests canbe accomplished. Enterobacteriaceae are gram-negative chemoautotrophs that possess both respiratory and fermentativemetabolism.

2 Glucose agar medium is used in the presumptive identification of Enterobacteriaceae based on the fermentationobserved in the medium (1). This medium is also recommended by ISO (2, 3) as a solid medium for the confirmation ofEnterobacteriaceae .The medium contains tryptone and yeast extract, which provides nitrogenous source and other essential growth factors. Sodiumchloride maintains the osmotic balance of the medium. Glucose in the medium provides the energy source and when fermentedproduces acid. The production of acid is indicated by yellow colour, as the indicator bromocresol purple turns yellow underacidic ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with agar gelColour and Clarity of prepared mediumPurple coloured, clear to slightly opalescent gel forms in Petri platesReactionReaction of w/v aqueous solution at 25 C.

3 PH : ResponseM1589: Cultural characteristics observed after an incubation at 35-37 C for 18-24 (CFU)GrowthRecoveryColour ofMediumEnterobacter aerogenesATCC 1304850-100luxuriant>=70%yellowHiMedia LaboratoriesTechnical DataEscherichia coli ATCC2592250-100luxuriant>=70%yellowPseud omonas aeruginosaATCC 2785350-100luxuriant>=70%colourlessStora ge and Shelf LifeStore below 30 C in tightly closed container and prepared medium at 2-8 C. Use before expiry period on the Corry J. E. L., Curtis G. D. W. and Baird R. M., Culture Media For Food Microbiology, Vol. 34, Progress in IndustrialMicrobiology, 1995, Elsevier, ISO 4702 Standard, 1993, Microbiology General Guidance For The Enumeration Of Enterobacteriaceae WithoutResuscitation3. ISO 8523 Standard, 1991, Microbiology General Guidance For The Detection of Enterobacteriaceae With : 2 / 2015 HiMedia Laboratories Pvt.

4 Ltd. A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-61471919 Email: :User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notbe considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.