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Glutathione Assay (Colorimetric) - G-Biosciences
Glutathione Assay (Colorimetric) (Cat. # 786 -075, 786 -076 ) Page 2 of 16 ... cytokine and immune response, protein glutathionylation and mitochondrial function and integrity. 3. Glutathione colorimetric assay kit is designed to measure redu ced glutathione (GSH),
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Biuret Protein Assay - G-Biosciences
www.gbiosciences.comIn the copper ion based protein assays, protein solutions are mixed with an alkaline solution of copp er salt, cupric ions (Cu 2+). The protein assay is based on the interaction of cupric ions with protein in an alkaline solution and is commonly referred to …
Bicinchoninic Acid (BCA) Protein Assay
www.gbiosciences.comSTANDARD PROTOCOL 1. Pipette 50µl of each standard and protein samples into an appropriately labeled tube. 2. Add 1ml Working Solution to each tube, seal and vortex to mix. 3. Incubate the assays at 37° C for 30 minutes or room temperature for 2 hours. For the enhanced protocol, incubate at 60°C for 60 minutes. We recommend a
Study of an Active Enzyme with Electrophoresis - G …
www.gbiosciences.comgelatin stock solution in both running and stacking gel solutions to a final concentration of 0.1% gelatin. Mix well before casting the gel. Mix well before casting the gel.
Plasmid Isolation (Alkaline Lysis) - G-Biosciences
www.gbiosciences.comTo pellet the plasmid DNA centrifuge at full speed for 15 minutes. 13. After centrifugation, examine the tubes fo r a small white pellet of plasmid DNA. Pour off the supernatants. 14. Add 300 µ l DNA Wash (70% isopropanol) to the pellets to wash away any excess salt. Centrifuge the tubes at full speed for 5 minutes. 15.
Polymerase Chain Reaction (PCR) - G-Biosciences
www.gbiosciences.comPolymerase Chain Reaction (PCR) Teacher s Guidebook (Cat. # BE æ305) ... In this experiment, students will amplify a nucleotide sequence from chromosome 16 to look for the insertion of a short DNA sequence called Alu within the PV92 locus.DNA from different individuals contain many regions that exhibit a great deal of diversity and ...
Bacterial Culture & Growth - G-Biosciences
www.gbiosciences.combacterial growth curve, the lag, log or exponential, stationary and the decline or death phase. x. LAG PHASE: Growth is slow at first, while the bacteria acclimatize to the nutrients and conditions in the growth media. x. LOG PHASE: Once acclimatized, …
Isolation &Characterization of Bacteria - G-Biosciences
www.gbiosciences.comAn interesting hands ron lab activity that teaches students the skills required for the isolation of bacteria from test samples. This kit teaches aseptic handing techniques and cultivation of bacteria. Using theses bacterial culture techniques students discover and isolate the bacteria present around us.
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