【技術資料】 GPC法 (SEC法)入門講座

1 TEL 059-364-5367 FAX 059-364-5258 1/6 GPC (SEC ) GPC (SEC ) GPC 1. GPC Gel Permeation Chromatography SEC (Size Exclusion Chromatography ) GFC Gel Filtration Chromatography SEC Size Exclusion GPC GFC SEC ( SEC ) 2. GPC SEC 1 GPC GPC (SEC ) 1) 1 ( ) TEL 059-364-5367 FAX 059-364-5258 2/6 1 2 3 4 1 GPC (PS) (PE)

2 2 PS PE + 2 GPC 3 3 + + + + +0815 20 25 30 35 ( )Log(M)108 PS107 PE+06106 04105 104 103 102 2 SEC TEL 059-364-5367 FAX 059-364-5258 3/6 3 4 4 4 5 5 3.

3 GPC 1 GPC TEL 059-364-5367 FAX 059-364-5258 4/6 1 (PS) THF, , , DMF, NMP (PMMA) HFIP, TFEA (PEO) , , , DMSO, GPC Mn Mw Mz (1) (3) N M C C C= M N iiiiii2iCMCNMNMMw ii2iii2ii3iMCMCNMNMMz iiiiiiMCCNNMMn (1) (2) (3) (1) Mn Mw Mz Mn + + + + +07 dw/d(Log(M))103104105106107 MnMwMzMp Mw Mz Mw + + + + +07 dw/d(Log(M))MnMwMzMp Mn Mw Mz ( 6)

4 Mn Mw Mz Mw Mn Mw/Mn Mz Mw Mz/Mw 3 103104105106107 6 ( ) (Mp ) TEL 059-364-5367 FAX 059-364-5258 5/6 GPC 7 + + + + +07 (%)103104105 + + + + +07 dw/d(Log(M))103104105 + + + + +07 (%)103104105 + + + + +07 (%)103104105 106107103104105 + + + + +07 dw/d(Log(M))103104105 + + + + +07 dw/d(Log(M))103104105 106107103104105 106107(a) (b) 7 1000 GPC 1 2 3 4 4 5 6 8

5 TEL 059-364-5367 FAX 059-364-5258 6/6 dw/dLog(M) 10152025 ( ) 10152025 ( ) + + + + +07 + + + + +07 1) 1991 2) , , Size Exclusion Chromatography , Springer (1999) 1) 3)Chi-San Wu, "Column Handbook for Size Exclusion Chromatography", Academic Press (1999) 4) , , , , "Modern Size-Exclusion Liquid Chromatography", Wiley (2009) 2D-LC 8 + + + + +07 (%) (%) + + + + +07 dw/d(Log(M)10152025 ( ) 2 10152025 ( ) + + + + +07 + + + + +07 + + + + +07 (%) 3 (%) + + + + +07 dw/d(Log(M) 4 + + + + +07 dw/d(Log(M) 6 5