Guide to Holding Times, Containers, and Preservatives ...

1 ParameterEPA Method(s)MinimumVolumeContainerTypeRequi redPreservativeHolding TimeContainerTypeRequiredPreservativeHol ding TimeDGAS - Methane, Ethane, Ethene, CO2 RSK SOP 175/147 3 X 40 mLVOA VialsIce7 / 14 Days CO2 only DGAS - Methane, Ethane, Ethene (w/o CO2)RSK SOP 175/147 3 X 40 mLVOA VialsHCl to pH<2 / Ice14 Days Explosives8330A 2 X 500 mLAmber GlassIce7E / 40A (Days)8 oz glass jarIce14E / 40A (Days)Glycols8015D 500 mLHDPE/GlassIce7E / 40A (Days)4 oz glass jarIce14E / 40A (Days)Herbicides8321B 500 mLAmber GlassIce7E / 40A (Days)4 oz glass jarIce14E / 40A (Days)PAH - Polynuclear aromatic Hydrocarbons625/8270D 2 X 500 mLAmber GlassIce7E / 40A (Days)4 oz glass jarIce14E / 40A (Days)Pesticides - Organochlorine andOrganophosphorus608/625/8270D 2 X 500 mLAmber GlassIce7E / 40A (Days)4 oz glass jarIce14E / 40A (Days)PCBs/Aroclors - Polychlorinated Biphenyls608/625/8082/8270 2 X 500 mLAmber GlassIce7E / 40A (Days)4 oz glass jarIce14E / 40A (Days)SVOC - Semi- volatile Organics (BNA)625/8270D 2 X 500 mLAmber GlassIce7E / 40A (Days)4 oz glass jarIce14E / 40A (Days)TPH - Total Petroleum HydrocarbonsTX 1005 3 X 40 mLVOA VialsHCl to pH<2 / Ice14 Days4 oz glass jarIce14 DaysTPH - Fractionation by TX 1006TX 1006 3 X 40 mLVOA VialsHCl to pH<2 / Ice14 Days after 1005E4 oz glass jarIce14 Days after 1005 ETPH-DRO - Diesel Range Organics8015D 2 X 500 mLAmber GlassHCl to pH<2 / Ice7E / 40A (Days)4 oz glass jarIce14E / 40A (Days)

2 Method 5035 Prep (BTEX-MTBE, GRO, VOC)5035 3 tared VOA VialsIce**Ship ASAP**BTEX-MTBE ( aromatic volatile Organics)602/624/8021/8260 3 X 40 mLVOA VialsHCl to pH<2 / Ice14 Days4 oz glass jarIce14 DaysTPH-GRO - Gasoline Range Organics8015D 3 X 40 mLVOA VialsHCl to pH<2 / Ice14 Days4 oz glass jarIce14 DaysVOC - volatile Organics by GC/MS624/8260C 3 X 40 mLVOA VialsUnpreserved / Ice7 Days - Full list+BTEX4 oz glass jarIce14 DaysVOC - volatile Organics by GC/MS624/8260C 3 X 40 mLVOA VialsHCl to pH<2 / Ice3 days - Acrylonitrile &Acrolein4 oz glass jarIce14 DaysVOC - volatile Organics by GC/MS624/8260C 3 X 40 mLVOA VialsHCl to pH<2 / Ice14 Days*4 oz glass jarIce14 DaysParameterEPA Method(s)MinimumVolumeContainerTypeRequi redPreservativeHolding TimeContainerTypeRequiredPreservativeHol ding 500 mLHDPE PlasticHNO3 to pH<2180 Days4 oz glass jarIce180 DaysDissolved Metals (Filtered in field/24 hours) 500 mLHDPE PlasticHNO3 to pH<2180 Days Hexavalent Chromium (Cr6) / Chromium VI7196A/SM3500-Cr D 250 mLHDPE PlasticIce**24 Hours**4 oz glass jarIce30E / 7A (Days) 500 mLHDPE PlasticHNO3 to pH<228 Days4 oz glass jarIce28 DaysHardness (as CaCO3) 500 mLHDPE PlasticHNO3 to pH<2 / Ice180 Days4 oz glass jarIce180 DaysIndividual MetalsPriority Pollutant MetalsRCRA 8 Metals(As, Ba, Cd, Cr, Hg, Pb, Se, Ag)Texas 11 Metals(As, Ba, Cd, Cr, Hg, Pb, Se, Ag + Sb, Be, Ni)TAL MetalsParameterEPA Method(s)

3 MinimumVolumeContainerTypeRequiredPreser vativeHolding TimeContainerTypeRequiredPreservativeHol ding TimeTCLP/SPLP Metals1311/6020A 1000 mLGlassIce6L&E / 6A (Months)8 oz glass jarIce6L&E / 6A (Months)TCLP/SPLP Mercury1311/7470A 1000 mLGlassIce28L&E / 28A (Days)8 oz glass jarIce28L&E / 28A (Days)TCLP/SPLP Semi-Volatiles / Pesticides1311/8270D 1000 mLGlassIce14L/7E/40A (Days)8 oz glass jarIce14L/7E/40A (Days)SPLP/ZHE Extraction VOC1311/1312 3 X 40 mLVOA VialsIce14L&E / 14A (Days)4 oz glass jarIce14L&E / 14A (Days)TCLP/SPLP Volatiles1311/8260C 3 X 40 mLVOA VialsIce14L&E / 14A (Days)4 oz glass jarIce14L&E / 14A (Days)RCI - Reactivity Corrosivity IgnitabilitySW846 Ch. 500 mLHDPE PlasticIce14 Days8 oz glass jarIce14 Days(Al, Sb, As, Ba, Be, Cd, Ca, Cr, Co, Cu, Fe, Pb, Mg, Mn, Hg, Mo, Ni, K, Se, Si, Ag, Na, Sr, Tl, Sn, Ti, V, Zn)WASTE CHARACTERIZATIONLIQUID/SLUDGESOIL/SOLIDM ETALSAQUEOUSSOILAQUEOUSSOILORGANICS(Sb, As, Be, Cd, Cr, Cu, Pb, Hg, Ni, Se, Ag, Tl, Zn)VOC HT - *14 days for pH<2 (Aq) except for Vinyl Chloride, Styrene, 2-CEVE.

4 Those compounds must be analyzed from an unpreserved VOA VOC HT - **Analysis must be completed 48 HOURS if samples are not frozen.** If Sample vials are frozen to < -7 C but above -20 C (or methanol), then HT is 14 days from to Holding Times, Containers, and Preservatives (Al, Sb, As, Ba, Be, B, Cd, Ca, Cs, Cr, Co, Cu, Fe, Pb, Li, Mg, Mn, Hg, Mo, Ni, K, Se, Si, Ag, Na, Si, Sr, Tl, Sn, Ti, U, V, Zn)ParameterEPA andStandard (SM)MethodsMinimumVolumeContainerTypeReq uiredPreservativeHolding TimeContainerTypeRequiredPreservativeHol ding 250 mLHDPE PlasticIce14 Days4 oz glass jarIce28 DaysAlkalinity (Bicarbonate, Carbonate) 250 mLHDPE PlasticIce14 Days4 oz glass jarIce28 F 250 mLHDPE PlasticH2SO4 to pH<2 / Ice28 Days4 oz glass jarIce28 DaysAnions (Br-Bromide, Cl-Chloride, F-Fluoride,SO4-Sulfate)300/9056A 250 mLHDPE PlasticIce (Preservation notrequired for Cl and F)

5 28 Days4 oz glass jarIce28 Days after EAnions (NO3-Nitrate, NO2-Nitrite, O-Phosphate)300/9056A 250 mLHDPE PlasticIce**48 Hours**4 oz glass jarIce48 Hours afterExtractionAnions (Combined Nitrate/Nitrite)300 only250 mLHDPE PlasticH2SO4 to pH<2 / Ice28 Days4 oz glass jarIce28 Days after EAnions (Br-Bromide, Cl-Chloride, F-Fluoride,SO4-Sulfate)620J 250 mLHDPE PlasticIceNo HT4 oz glass jarIce28 Days after EConductivity / Specific 250 mLHDPE PlasticIce28 Days4 oz glass jarIceNo HTChemical Oxygen Demand (COD)HACH 8000/SM5220D250 mLHDPE PlasticH2SO4 to pH<2 / Ice28 Days28 DaysCyanide, Total or E&G 250 mLHDPE PlasticNaOH to pH>12 / Ice14 Days4 oz glass jarIce14 DaysCyanide and Sulfide, ReactiveSW846 Ch. 500 mLHDPE PlasticIce14 Days4 oz glass jarIce14 DaysFerrous Iron (Fe) 3 X 40 mLVOA VialsIce7 Days Ignitability1010A 500 mLHDPE PlasticIce6 Months4 oz glass jarIce28 DaysMoisture / Percent Moisture (Dry Weight)

6 ASTM D2216-05 4 oz glass jarIceNot RegulatedpH / + B 40/250 mLAmber GlassIce**ASAP**4 oz glass jarIce**ASAP**Oil and Grease, Hexane Extractable Material1664A / 90701000 mLAmber GlassH2SO4 to pH<2 / Ice28 Days Perchlorate332/6860A250 mLHDPE PlasticIce28 Days4 oz glass jarIce28 DaysPhosphorus, E 250 mLHDPE PlasticIce**48 Hours**4 oz glass jarIce14 DaysPhosphorus, A 250 mLHDPE PlasticH2SO4 to pH<2 / Ice28 Days4 oz glass jarIce28 DaysSilica, 8185 250 mLHDPE PlasticIce28 Days4 oz glass jarIce28 DaysSolids, Total Dissolved (TDS) mLHDPE PlasticIce7 Days Solids, Total Suspended (TSS) mLHDPE PlasticIce7 Days Solids, Settleable (SETSOLID)SM2540F1000 mLHDPE PlasticIce**48 Hours** Sulfide, 4500-S2 D 250 mLHDPE PlasticNaOH+ZnAc / Ice7 Days4 oz glass jarIce14 DaysTotal organic Carbon (TOC) 3 X 40 mLVOA VialsH3PO4 ,HCl or H2SO4 topH<2 / Ice28 Days4 oz glass jarIce28 250 mLHDPE PlasticIce**48 Hours** Containers: Preservatives :Hold Time(from Date/Time Collected):L - From Field Collection to TCLP Extraction, E - Extraction, A - AnalyzeJohn DuPont - General Receipt / contact DHL Analytical with your Environmental Analytical Needs.

7 If TSS/TDS/Settleable Solids is also requested, then use 1000 mL HDPE Plastic more than one Wet Chemistry Test is requested, then use 500 mL HDPE Plastic - Hydrochloric Acid, H2SO4 - Sulfuric Acid, HNO3 - Nitric Acid, NaOH - Sodium Hydroxide, ZnAc - Zinc AcetateSTORAGE REQUIREMENTS: Samples must arrive at laboratory on ice OR be stored at 6 Vial - volatile organic Analyte (40 mL screw-top vial), HDPE - High Density PolyethyleneWET CHEMISTRYAQUEOUSG uide to Holding Times, Containers, and PreservativesSOILA bbreviations.