HiCrome Candida Differential Agar - HiMedia Labs

1 Please refer disclaimer Candida Differential AgarM1297 AIntended UseHiCrome Candida Differential agar is recommended for rapid isolation and identification of Candida species from mixed cultures in clinical and non-clinical **IngredientsGms / LitrePeptone, hydrogen pH ( at 25 C) **Formula adjusted, standardized to suit performance parametersDirectionsSuspend grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45-50 C. Mix well and pour into sterile Petri And InterpretationPerry and Miller (3) reported that Candida albicans produces an enzyme b -N-acetyl- galactosaminidase and according to Rousselle et al (4) incorporation of chromogenic or fluorogenic hexosaminidase substrates into the growth medium helps in identification of isolates directly on primary isolation.

2 HiCrome Candida Differential agar is a selective and Differential medium, which facilitates rapid isolation of yeasts from mixed cultures and allows differentiation of Candida species namely , , and on the basis of colouration and colony morphology. On this medium results are obtained within 48 hours and it is useful for the rapid and presumptive identification of common yeasts in Mycology and Clinical Microbiology special and yeast extract provides nitrogenous, carbonaceous compounds and other essential growth nutrients. Phosphate buffers the medium well. Chloramphenicol suppresses the accompanying bacterial flora. appear as light green coloured smooth colonies, appear as blue to metallic blue coloured raised colonies. colonies appear as cream to white smooth colonies, while appear as purple fuzzy colonies.

3 Type of specimen Clinical samples - skin scrapings, Collection and HandlingFor clinical samples follow appropriate techniques for handling specimens as per established guidelines (3,4). After use, contaminated materials must be sterilized by autoclaving before and PrecautionsIn Vitro diagnostic Use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data in colour intensity may be observed for Candida isolates depending on the presence of Other Candida species may produce light mauve coloured colonies which is also produced by other yeast cells.

4 This mustbe confirmed by further biochemical Other filamentous fungi also exhibit colour on this LaboratoriesTechnical DataCandida albicans ATCC10231 (00054*)50-100good-luxuriant>=50%light greenCandida glabrata ATCC1512650-100good-luxuriant>=50%cream to white#Teunomyces krusei ATCC2440850-100purple, fuzzyCandida tropicalis ATCC75050-100good-luxuriant >=50%good-luxuriant >=50%blue to purpleEscherichia coli ATCC25922 (00013*)>=104inhibited0%>=104inhibited0% Store dehydrated powder and prepared medium at 2-8 C. Use before expiry period on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use.

5 Use before expiry date on the label. Product performance is best if used within stated expiry period. Quality ControlAppearanceCream to beige homogeneous free flowing powderGellingFirm, comparable with agar gelColour and Clarity of prepared mediumLight amber coloured, clear to slightly opalescent gel forms in Petri platesReactionReaction of w/v aqueous solution at 25 C. pH : ResponseM1297A: Cultural characteristics observed after an incubation at 30-35 C for 40-48 (CFU)GrowthRecoveryColour ofColonyPerformance And EvaluationPerformance of the medium is expected when used as per the direction on the label within the expiry period when stored atrecommended and Shelf lifeUser must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product.

6 Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (1,2). DisposalKey : *Corresponding WDCM numbers. # - Formerly known as Candida krusei Candida kefyr ATCC 6605850-100cream to white with slight purple centregood-luxuriant >=50% Candida utilis ATCC 995050-100pale pink to pinkish purple good-luxuriant >=50% Candida parapsilosis ATCC 2201950-100white to creamgood-luxuriant >=50% Candida membranifaciens ATCC 2013750-100white to creamgood-luxuriant >=50% Candida dubliensisNCPF 394950-100pale greengood-luxuriant >=50%Staphylococcus aureus ATCC 25923 ( 00034*) HiMedia LaboratoriesTechnical DataDisclaimer :User must ensure suitability of the product(s) in their application prior to use.

7 Products conform solely to the information contained inthis and other related HiMedia publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notbe considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any vitro diagnostic medical device CE MarkingDo not use if package is damagedCE Partner 4U ,Esdoornlaan 13, 3951 DB Maarn The Netherlands, IVDS torage temperature2 C8 CEC REPHiMedia Laboratories Pvt.

8 Limited, 23 Vadhani Industrial Estate, LBS Marg,Mumbai-86,MS,India HiMedia Laboratories Pvt. Ltd. : 23, Vadhani , LBS Marg, Mumbai-400086, India. Customer care No.: 022-6116 9797 Corporate office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email: Website: P., Freydiere A., Couillerot P., de Montclos H. and GilleY., 1994, J. Clin. Microbiol. 32 : 04/ , Clinical Microbiology Procedures Handb0ook. 2nd , , Pfaller , , Carroll, , Funke, G., Landry, , Richter, and Warnock., (2015) Manualof Clinical Microbiology, 11th Edition. Vol. J. L. and Miller G. R., 1987, J. Clin. Microbiol., 25: 2424 -2425.