HPLC Column Protection UHPLC HPLC PREP - …

1 UHPLCHPLCPREP Universal Column Protection hplc Column ProtectionLess frequent UHPLC / hplc / prep Column replacementSave Time and MoneyUHPLC / hplc / prep 2It s a fact! Chemical contaminants and particu-lates are a natural part of any chromatographic easiest way to extend Column performance is to remove these contaminants and particulates with the SecurityGuard Cartridge System before they reach your Column and degrade your : Protects and extends UHPLC , hplc and prep Column lifetime Virtually no change in chromatography Simple to useScanning electron microscopy of Column inlet fritWith SecurityGuard ULTRAW ithout SecurityGuard ULTRA(24000 times magnification)(24000 times magnification) 2016 Phenomenex, Inc.

2 All rights FritNo contaminant or particle buildupColumn MediaIntact mediaContaminant and particle buildupColumn MediaCrushed media and finesInlet FritPhenomenex l WEB: Your Column ProtectionNo matter which type of Column you re using, SecurityGuard offers the Column Protection you needSecurityGuard ULTRAAll core-shell and/or < 3 m particle columns (< 20,000 psi / 1,378 bar)pp. 4 - 9 SecurityGuard PREPFor prep hplc columns with to mm internal diameter (ID)pp. 16 - 18 SecurityGuard StandardAll non core-shell and 3 m particle columns (< 3,500 psi / 241 bar)pp.

3 10 - 15 UHPLCU ltra-High Performance Liquid ChromatographyPREPP reparative hplc HPLCHigh Performance Liquid ChromatographyUHPLC Column ProtectionUHPLC: SecurityGuard ULTRA4 UHPLC Column Protection : Extends UHPLC , core-shell, and/or < 3 m Column lifetime Fits virtually all manufacturers columns to mm ID Pressure rated to 20,000 psi (1,378 bar) Simple to useThe SecurityGuard ULTRA cartridge traps contaminants and particulates. Once the cartridge is exhausted, simply replace it instead of your expensive Column universal holder provides a leak-free connection to virtually any UHPLC will experience.

4 Increased Column lifetime Higher Column performance More reproducible chromatography Fewer wasted columns* Cartridge schematic not drawn to scaleSecurityGuard ULTRAUHPLC Column to DetectorFrom InjectorCartridgeHolderMaterial ChamberFritCompounds of InterestContaminants and Particulates2012 R&D 100 Award RecipientRemoves harmful contaminants and particulatesLonger Column LifetimeContaminants TrappedPhenomenex l WEB: Column ProtectionUHPLC: SecurityGuard ULTRA5 The SecurityGuard ULTRA Column Protection system is designed to extend the lifetime of < 3 m and/or core-shell columns, while main-taining the ultra-high performance results they R&D 100 Award RecipientPressure rated to 20,000 psi Specifically for UHPLC columns and methodsEasy to Use.

5 Innovative DesignCartridgeHolderAuto-adjusting Tipprovides a leak-free connection to virtually any UHPLC columnDisposable Cartridgefor quick and easy removal and replacementPacked with Mediafor maximum Column protectionConveniently Labeledto ensure guard-to- Column phase compatibilityRe-usable Holderminimizes costs and wastePhenomenex l WEB: Column ProtectionUHPLC: SecurityGuard ULTRA6 Keeps UHPLC columns performing at their bestOne Easy StepWhen contaminates and particulates build up at the head of the Column or on the guard cartridges, system pressures dramatically simply replacing the SecurityGuard ULTRA cartridge instead of your < 3 m and/or core-shell UHPLC Column , you are able to regain normal operating conditions and reclaim original Column this accelerated Column lifetime test, the UHPLC Column lasts substantially longer with SecurityGuard ULTRA guard cartrdige (Bar)

6 No Guard10005010015020025030012014016018020 0220240260280300 Cartridge ReplacementCartridge Replacement2012 R&D 100 Award RecipientWatch how easy it is to replace your SecurityGuard ULTRA Performance Accelerated lifetime test using endogenous biological matrix on Kinetex m C18 50 x mm IDWith Guardwith SecurityGuard ULTRANo GuardPhenomenex l WEB: Column ProtectionUHPLC: SecurityGuard ULTRA7 Universal connectorProtect Any UHPLC ColumnInstalling the SecurityGuard ULTRA Protection system into your favorite UHPLC Column is straightforward and floating outlet from the SecurityGuard ULTRA universal holder automatically adjusts to the precise depth of virtually every manufacturer s analytical UHPLC Column .

7 This ensures a low dead-volume, leak-free connection, without the use of unions or adapters, no matter which manufacturer s < 3 m and/or core-shell UHPLC Column you are UHPLC Fittings2012 R&D 100 Award RecipientTip adjusts to fit anyUHPLC columnPhenomenex l WEB: Column ProtectionUHPLC: SecurityGuard ULTRA8 Virtually no change in chromatographyMaintain UHPLC Performance!SecurityGuard ULTRA maintains UHPLC Column performance without altering your chromatographic results.

8 The extremely low dead-volume (< L) of this unique guard design minimizes sample peak disper-sion ensuring that ultra-high efficiency chromatography is competitors UHPLC guard systemsSecurityGuard ULTRA has virtually no effect on efficiency, retention time, or backpressure. This leads to longer Column lifetime with no loss in Column same for all applications: Column : Kinetex m XB-C18 Dimensions: 50 x mmMobile Phase: Acetonitrile / Water (50:50)Injection Volume:1 LFlow Rate: 1 mL/minTemperature:22 CDetection: UV @ 270 nmSample:1. Acetophenone2.

9 Benzene3. Toluene 4. NaphthaleneSecurityGuard ULTRA outperforms the competitionComparative separations may not be representative of all GuardSecurityGuard ULTRA CompetitorEfficiency (p/m) 11,79511,3908, (bar) 354384384 Values based on naphthalene ID 19973 APP ID 19974 APP ID 19976 SecurityGuard ULTRA CompetitorNo Guard Phenomenex l WEB: Column ProtectionUHPLC: SecurityGuard ULTRA9 SecurityGuard ULTRAO rdering Information2012 R&D 100 Award RecipientDesigned for all core-shell and/or < 3 m particle columns (< 20,000 psi / 1,378 bar)See it in ULTRA Cartridges Column ID (mm) MaterialDescriptionpH Stability for General Purpose/ Pharmaceutical /3 pk /3 pk /3 pkEVO C18(ODS, Octadecyl) (ODS, Octadecyl) * AJ0-8782AJ0-8775AJ0-8768C8(MOS, Octyl) * AJ0-8784AJ0-8777AJ0-8770 PFP(Pentafluorophenyl) (Pentafluorophenyl)

10 (Biphenyl) * (Phenylhexyl) * AJ0-8788AJ0-8781AJ0-8774 Cartridges for General Purpose/ Pharmaceutical (Fully Porous Columns)For fully porous columns like Luna Omega (Phenomenex)C18(ODS, Octadecyl) *AJ0-9502 Polar C18(ODS, Octadecyl) *AJ0-9505 Cartridges for Protein and Peptide Reversed PhaseFor use with columns like Aeris (Phenomenex)Widepore C18(ODS, Octadecyl) *AJ0-8783 AJ0-8769 Widepore C8(MOS, Octyl) *AJ0-8785 AJ0-8771 Widepore C4(Butyl) *AJ0-8899 AJ0-8901 Peptide C18(ODS, Octadecyl) *AJ0-8948AJ0-8947AJ0-8946 Cartridges for Synthetic DNA / RNA AnalysisFor use with columns like Clarity (Phenomenex)Oligo-MS C18(ODS, Octadecyl) *AJ0-9068 AJ0-9066 Oligo-XT(ODS, Octadecyl) AJ0-9514 Cartridges for Silica GFC (Gel Filtration Chromatography)(Aqueous SEC) For use with silica GFC columns such as Yarra (Phenomenex)X150 AJ0-9512X300 AJ0-9513* pH stable - under gradient conditions.