Transcription of I. 土壌の生物性評価に係る技術 - maff.go.jp
1 45 I.
2 DNA 46 9 DNA 44 DNA 45 DNA 20 44 High throughput 45 1 47 21.
3 In situ FISH FISH Fluorescence in situ hybridization 1 46 46 DNA 48 .. PCR 47 PCR 1 DNA RNA PCR PCR PCR 22 Ct Threshold Cycle DNA Ct PCR 48 22 PCR PCR 47 48 PCR 49.
4 DNA DNA DNA DNA 23 thermo Fisher scientific 50 24 thermo Fisher scientific .. / 49 / 50 / / / 49 50 m nm 51 25 /.
5 51 1nm 52 1 DNA RNA 51 52 2 1nm 2 52 26 1 , , p396-402 2016 27 1 1 , , p396-402 2016 53 .. DGGE Denaturing gradient gel electrophoresis, 53 DGGE DNA DGGE DNA PCR PCR-DGGE 16 SrRNA PCR-DGGE 28 DNA PCR-DGGE ) 53 54.
6 29 .. 55 30 SYBR GreenI ( ) .. eDNA eDNA eDNA DAPI 54 eDNA eDNA DNA 54 DAPI 56 31 DAPI eDNA SOFIX SOFIX.
7 2010 55 DGC CO2 56 CO2 55 56 57 32 GN2 57 Biolog 52 , 8 , p679-683 2013 .. 58 DNA 57 58 58 33 # 34 mmol 25 C 1 2 3 2008 59.
8 59 Biolog 60 96 Biolog Biolog Biolog 1 100 1,000 Biolog 1 2 61 35 JST 2019 1 29 1 59 60 Biolog Biolog 61 Tryptophan Methionine 60.
9 62 63 62 63 61 36 OTU 64
10 OTU 32 2 , p51-57(2017) 64 Operational taxonomic unit OTU
