ICS-3000 Ion Chromatography System Operator's …

1 ICS- 3000 Ion Chromatography SystemOperator's ManualDocument No. 065031 Revision 01 May 2005 2005 by Dionex Corporation All rights reserved worldwide. Printed in the United States of publication is protected by federal copyright law. No part of this publication may be copied or distributed, transmitted, transcribed, stored in a retrieval System , or transmitted into any human or computer language, in any form or by any means, electronic, mechanical, magnetic, manual , or otherwise, or disclosed to third parties without the express written permission of Dionex Corporation, 1228 Titan Way, Sunnyvale, California 94088-3603 OF WARRANTY AND LIMITED WARRANTYTHIS PUBLICATION IS PROVIDED AS IS WITHOUT WARRANTY OF ANY KIND. DIONEX CORPORATION DOES NOT WARRANT, GUARANTEE, OR MAKE ANY EXPRESS OR IMPLIED REPRESENTATIONS REGARDING THE USE, OR THE RESULTS OF THE USE, OF THIS PUBLICATION IN TERMS OF CORRECTNESS, ACCURACY, RELIABILITY, CURRENTNESS, OR OTHERWISE.

2 FURTHER, DIONEX CORPORATION RESERVES THE RIGHT TO REVISE THIS PUBLICATION AND TO MAKE CHANGES FROM TIME TO TIME IN THE CONTENT HEREINOF WITHOUT OBLIGATION OF DIONEX CORPORATION TO NOTIFY ANY PERSON OR ORGANIZATION OF SUCH REVISION OR , Atlas, Chromeleon, EluGen, IonPac, OnGuard, and SRS are registered trademarks of Dionex Corporation. CarboPac, MicroMembrane, MMS, Reagent-Free, and RFIC are trademarks of Dionex Corporation. Adobe, Adobe Reader, and Acrobat are registered trademarks of Adobe Systems, Incorporated. Kel-F is a registered trademark of 3M Corporation. FastLock and MSQ Plus are trademarks of Thermo Electron Corporation. PEEK is a trademark of Victrex PLC. PharMed is a registered trademark of Saint-Gobain Performance Plastics. Teflon is a registered trademark of duPont de Nemours & HISTORYR evision 01, May 2005 Doc. 065031-01 5/05 iContents1 System Overview.

3 System Components .. System Control .. System Documentation .. ICS- 3000 System operator s manual .. Messages and Notes .. and Regulatory Information .. Labels .. 132 DescriptionDP/SP Front Features .. Interior Components .. Heads .. Transducer .. Valves (Gradient pump only) .. Degassing Module .. Seal Wash System .. Mixer .. 20 ICS- 3000 Ion Chromatography Systemii Doc. 065031-01 5 Flow Schematics .. Pump Flow Schematic .. Pump Flow Schematic .. Rear Panel .. Reservoirs .. (Optional) ..28EG Front Features .. Interior Components .. Rear Panel .. EG Flow Schematic ..37DC DC Front Features .. DC Interior Components .. DC Rear Panel .. Injection Valves .. Injection Valve Operation .. Injection Valve Plumbing .. CD Conductivity Detector.

4 Heated Conductivity Cell .. Suppressor .. System Flow Schematic for Conductivity Detection .. ED Electrochemical Detector .. Amperometry Cell ..54 ContentsDoc. 065031-01 5/05 Combination pH Ag/AgCl Reference Electrode .. DC Amperometric Detection .. Integrated and Pulsed Amperometric Detection .. Cyclic Voltammetry Detection .. Waveforms .. Storing and Reprocessing Amperometry Data .. Automation Manager .. High-Pressure Switching Valves .. Low-Pressure Valves .. High- and Low-Pressure Valve Control .. RCH-1 Reaction Coil Heater .. I/O Option .. I/O Option Connections .. Analog Outputs .. Power, Relay, and TTL Outputs .. TTL Inputs .. 833 ICS- 3000 .. 874 Precautions .. Operating Precautions .. Amperometry Cell Operating Precautions.

5 97 ICS- 3000 Ion Chromatography Systemiv Doc. 065031-01 5 Startup Checklist .. Samples .. and Storing Samples .. Samples .. Samples .. the Autosampler Vials and Load the Sample Tray ..102DP/SP Up the Eluent Reservoir(s) .. Up the Piston Seal Wash System .. the Pump ..105EG the Eluent Concentration ..109DC the DC .. the System and Verify Operation Status ..1135 .. Samples .. Samples with a Syringe (Push Method) .. Samples with a Vacuum Syringe (Pull Method) .. Samples with an Autosampler .. Samples ..119 ContentsDoc. 065031-01 5/05 Commands for Loading and Injecting Samples .. for an AS Autosampler .. for an AS40 Autosampler .. Sample Processing .. manual Data .. (Batch) Sample Processing .. a New Sequence .. 1236 ShutdownDP/SP ShutdownEG Shutdown.

6 Shutdown .. 127DC Storage .. Cell Storage .. Storage of the Amperometry Cell .. Storage of the Amperometry Cell .. 1297 Maintenance Checklists .. Maintenance .. Maintenance .. 131 ICS- 3000 Ion Chromatography Systemvi Doc. 065031-01 5 Maintenance .. Maintenance ..132DP/SP Routine Daily Maintenance .. Weekly Maintenance .. Periodic Maintenance .. Annual Maintenance ..134EG Routine Daily Maintenance .. Weekly Maintenance .. Annual Maintenance ..135DC Daily Maintenance .. DC Weekly Maintenance .. DC Periodic Maintenance .. DC Annual Maintenance ..1378 Trail Error Messages .. Error Messages .. Error Messages .. Error Messages .. Baseline ..143 ContentsDoc. 065031-01 5/05 Retention Time Reproducibility .. Retention Times Are Too Early.

7 Retention Times Are Too Late .. Peaks .. Peaks .. System Backpressure .. System Backpressure .. Low Detector Output .. High Background .. 151DP/SP Troubleshooting DP/SP Error Messages .. DP/SP Does Not Start .. DP/SP Stops .. DP/SP Liquid Leaks/Leak Alarm .. Vacuum Degassing Module Low Vacuum .. Vacuum Degassing Module Does Not Run .. DP/SP Digital I/O Port Inoperative .. 165EG Troubleshooting EG Error Messages .. EG ALARM LED Is Lighted .. EG POWER LED Fails to Light .. Liquid Leaks in the EG .. No Flow .. EG Stops Operation .. 174 ICS- 3000 Ion Chromatography Systemviii Doc. 065031-01 5/05DC Troubleshooting DC Error Messages .. Liquid Leaks from DC Components .. Amperometry Cell Troubleshooting .. Amperometry Cell pH Readout Always.

8 Cannot Set Amperometry Cell pH Readout to .. Shift in Amperometry Cell pH Readout .. No Amperometry Cell pH Readout or Intermittent Readout . Leak in pH Reference Electrode Compartment .. Shift in Ag/AgCl Reference Potential ..1889 ServiceDP/SP Tubing and Fittings .. Eluent Reservoirs .. the DP/SP .. with the PUMP PRIME Button .. from the Control Panel .. the Check Valve Cartridges .. a Piston Seal .. the Pump Head and Piston .. the Piston .. the Main Piston Seal .. the Seal Wash Piston Seal ..201 ContentsDoc. 065031-01 5/05 the Piston, Piston Seals, and Pump Head .. the Piston .. the Pump Head and Piston .. the New Piston .. the Pump Head .. the Piston Seal Wash Tubing .. the DP/SP Priming Valve Knob Seal .. the DP/SP Main Power Fuses .. 207EG Replacing Tubing and Fittings.

9 Isolating a Restriction in the Liquid Lines .. Replacing the EluGen Cartridge .. Removing the Old EluGen Cartridge .. Disposing of the Old EluGen Cartridge .. Installing the New EluGen Cartridge .. Conditioning the New EluGen Cartridge .. Replacing the CR-TC .. Removing the Old CR-TC .. Installing and Hydrating the New CR-TC .. Completing the CR-TC Plumbing .. Replacing the RFIC Eluent Degasser .. Installing a Backpressure Coil .. Changing the EG Main Power Fuses .. 225 ICS- 3000 Ion Chromatography Systemx Doc. 065031-01 5/05DC Replacing Tubing and Fittings .. Replacing the Leak Sensor .. Rebuilding a High-Pressure (Injection) Valve .. Replacing a High-Pressure (Injection) Valve .. Installing or Replacing an I/O Option Board .. Changing the DC Main Power Fuses.

10 Wellness Panel Overview .. CD Service Procedures .. Calibrating the CD Cell .. Replacing a Suppressor .. Replacing a CD .. Removing Trapped Air from the Conductivity Cell .. ED Service Procedures .. Disconnecting the Amperometry Cell .. Replacing an Amperometry Cell Gasket .. Polishing an Amperometry Cell Working Electrode .. Replacing an Amperometry Cell Reference Electrode .. Calibrating the Reference Electrode .. Replacing the Reference Electrode O-Ring .. Replacing an ED ..255 ContentsDoc. 065031-01 5/05 xiA SpecificationsDP/SP .. Connections .. Interfaces and Inputs/Outputs .. 259EG .. EluGen Cartridge .. 262DC Electrical .. Environmental .. Physical .. Lower Compartment Components .. Temperature Control .. Upper Compartment.