IFA kit for antinuclear antibodies FLUORO HEPANA …

1 FLUORO HEPANA TESTIFA kit for antinuclear antibodiesScreening of autoimmune diseases Functional analysis of antigens For in vitro diagnostic use onlyMBL has conducted annual quality control survey for customers in the world. Our customer service can assist you when you have difficulties in interpreting patterns. FLUORO HEPANA TEST provides you with visible and a wide range of information on antinuclear envelopeNucleolarPeripheralDiscrete-Spec kledSpeckledPCNAR ibosomalActinSpindleGolgi apparatusLysosomeCentrioleStaining patterns of FLUORO HEPANA TESTI nterphaseMitotic phaseCytoplasmKaryoplasmChromatinNucleol usNuclear envelopeCytoplasmKaryoplasmChromosomeHEp -2 cellsIn the FLUORO HEPANA TEST, HEp-2 cells which are cultured cells derived from human pharyngeal cancer are used as substrate. Since the nuclei of HEp-2 cells are large, the staining pattern can be observed in detail. Moreover, since cells in various stages of the cell cycle coexist, FLUORO HEPANA TEST makes it possible to detect anti-centromere antibodies and anti-PCNA (proliferating cell nuclear antigens) nucleus is stained smoothly, primarily around the outer region of the nucleus, with weaker staining toward the center of the nucleus.

2 In a mitotic cell, chromosome region is strongly antibody: Anti-DNA Associated disease: SLEE ntire nucleus is stained homogeneously and smoothly. Chromosome region in a mitotic cell shows the same or brighter fluorescence than that in interphase antibody: Anti-histone, Anti-DNPA ssociated disease: SLE, Drug-induced lupus, RA, SjSInterpretation of Results(-): No specific fluorescence is detected in cell nucleus.( ): Although slight staining is detected in cell nucleus, staining pattern cannot be identified.(+): Specific Fluorescence is clearly detected throughout the entire nucleus, or in a certain area of the patternPositive staining patterns are mostly classified as follows. In some cases, multiple staining patterns patternHomogeneous patternNo significant nuclear and cytoplasmic staining. Depending on the sensitivity of the fluorescence microscope, faint fluorescence can be observed, which is considered negative. SLE:systemic lupus erythematosus RA: rheumatoid arthritis SjS:Sj gren s syndrome SSc: systemic sclerosisMCTD:mixed connective tissue disease PBC:primary biliary cirrhosis Speckled patternNucleolar patternDiscrete-speckled patternAnti-PCNA antibodyGrainy fluorescence is detected inside nucleus, and staining is not smooth.

3 The nucleoli are not usually stained. The chromosome region in a mitotic cell is not antibody: Anti-ENA (Sm, RNP, SS-A, SS-B, etc.)Associated disease: SLE (anti-Sm), MCTD (anti-RNP), SjS (anti-SS-B)Nucleolus is stained as several large dots or clumps of granules inside nucleus. Usual number of dots is less than 6 per antibody: Anti-NucleolarAssociated disease: SSc, SLE, SjSHomogeneously distributed and speckled fluorescence in nucleus is observed. The number of speckles is usually 40-60 grains per nucleus. Mitotic figures discrete speckles over the chromosome region. Present antibody: Anti-CentromereAssociated disease: SSc (CREST)When positive cells and negative cells coexist, the presence of anti PCNA antibody is suspected. Staining pattern varies depending on cell cycle. Associated disease: SLEWhen staining is observed in cytoplasm, the presence of anti-mitochondria antibody or anti-smooth muscle antibody is suggested. Cytoplasm is stained in a granule-like-pattern with anti-mitochondria antibody and a fibrous pattern with anti-smooth muscle antibody.

4 In this case, this kind of antibodies should be confirmed using substrates such as stomach or kidney of rat. (MBL AID-1 TEST, )Associated disease: PBCAlso, the following staining pattern is observed for each of the antibody antibodies Diseasesanti-dsDNA SLEanti-ssDNA SLE, Other collagen diseasesanti-DNA-Histone SLE, Autoimmune Hepatitisanti-Histone SLE, Drug-induced Lupusanti-Centromere SSc (CREST), PBCanti-Nucleolar SScanti-RNP MCTD, SLE, UCTD anti-Sm SLEanti-SS-A/Ro SjS, SLEanti-SS-B/La SjSanti-Scl-70 SSc (diffuse scleroderma)anti-Jo-1 PM/DManti-PCNA SLESLE:systemic lupus erythematosus SSc: systemic sclerosisPBC:primary biliary cirrhosis MCTD:mixed connective tissue diseaseUCTD:unclassified connective tissue disease SjS:Sj gren s syndromePM/DM.

5 Polymyositis/dermatomyositis ANAs and associated diseasesRelated ProductELISA test for detection of Disease Specific ANAMESACUP ANA TEST7560E 96 tests 2-8 C Product Code No. Quantity Storage FLUORO HEPANA TESTHEPANA TEST SlideHEPASERA-1 HEp-2 slideFITC conjugated goat anti-human immunoglobulinsPBS buffer, ANA positive control serumMounting medium, Cover slip, Blotting paperProduct Code No. Quantity Storage Kit components4210E 80 wells 2-8 C4220E 160 wells 4220-12E 240 wells 4211E 4 wells 40 slides 2-8 C 4221E 8 wells 40 slides4200E mL 4 2-8 CControl Sera for IF ANA detectionOne vial each of Homogeneous, Speckled, Nucleolar, Discrete-speckledHEp-2 slide341460-0031010 NMEDICAL & BIOLOGICAL LABORATORIES CO.

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