Transcription of 幹細胞と細胞療法 III 2．前立腺における幹細胞・前駆細胞と …
1 III 2 . 2 .. Prostate Stem PSCA . Cell Antigen ; PSCA .. PSCA GFP. PSCA GFP GFP. GFP . GFP 5 . 14 . PSCA GFP . GFP .. GFP . PSCA GFP TRAMP. GFP 23 PSCA . GFP GFP . GFP PSCA GFP .. Visualization of prostatic stem progenitor cells during development, regeneration and tumorigenesis TETSURO WATABE Department of Molecular Pathology, Graduate School of Medicine, University of Tokyo . Key words .. University . of California , Post-doctral fell- . ow 13 .. 90 117 . 2 .. 3 .. 2 .. 1 .. Bonkhoff . 3 1 .. 1 .. 1 .. 3 . 9 .. 2 .. 91. PSA- PSA+. bcl-2+ bcl-2- .. PSA- chromogranin+.. PSA++.. bcl-2- .. PSA+ . bcl-2+.. PSA- chromogranin+.. 1 .. 5,6 2 A .. Prostate Stem Cell Anti- . gen PSCA PSCA PSCA GFP . GFP . 8 .. 2 B GFP . 4 . PSCA .. GFP Green Fluores- cence Protein.
2 92 117 . Transillumination Image Green Fluorescence Image 2 PSCA GFP GFP . A . B .. 4 GFP . 3 B .. 8. PSCA GFP GFP 3 C 14 3 D .. GFP .. GFP .. 5 GFP . 3 .. 1 . GFP . 3 A GFP . PSCA GFP . GFP . 3 8 14 PSCA GFP.. GFP GFP 4 A . 4 . 93. A. 1 week old B. 5 week old C. 8 week old D. 14 week old 3 GFP . P mm 4 GFP . B PSCA GFP 4 C GFP 5 .. 4 D GFP .. PSCA GFP . PSCA GFP 1 GFP . GFP . 1 . GFP 1 . 1 2 . 94 117 . A. Control B. Castrated 2 wks . C. Castrated 2 wks D. Castrated 2 wks . +Androgen 1 wk +Androgen 5 wks . TI GF TI GF. 4 GFP .. 3 PSCA GFP GFP .. 3 GFP . GFP .. SV 40 T. antigen . 6 GFP 7 . TRAMP PSCA GFP.. TRAMP . 3 .. TRAMP PSCA GFP T G .. TRAMP PSCA GFP T . 95. T G . TRAMP TRAMP GFP 5. GFP GFP . B, C GFP .. GFP T G . GFP . T G . GFP .. PSCA GFP.
3 GFP .. GFP .. GFP .. 1 Reiter R, Witte O . 5 GFP .. 2000 ; 19 : 414 419. A BL PR SV B 2 English HF, Santen RJ, Isaacs JT : Response of glan- C dular versus basal rat ventral prostatic epithelial cells to androgen withdrawal and replacement . Prostate 1987 ; 11 : 229 242. 3 Bonkhoff H, Remberger K : Differentiation pathways and histogenetic aspects of normal and abnormal prostatic growth : a stem cell model. Prostate 1996 ;. G GFP . 28 : 98 106. 20 25 4 Reiter RE, Gu Z, Watabe T, et al. : Prostate stem cell . 5 A T G antigen : a cell surface marker overexpressed in pros- . tate cancer. Proc Natl Acad Sci USA 1997. 5 Sugimura Y, Cunha GR, Donjacour AA, et al. : Whole- mount autoradiography study of DNA synthetic activ- T.. ity during postnatal development and androgen- G GFP induced regeneration in the mouse prostate.
4 Biol Re- prod 1986 ; 34 : 985 995.. 96 117 . 6 Lee C, Sensibar JA, Dudek SM, et al. : Prostatic ductal fetus . system in rats : regional variation in morphological and hor- functional activities. Biol Reprod 1990 ; 43 : 1079 . 1086. mone dependent . 7 Greenberg NM, DeMayo F, Finegold MJ, et al. : Pros- . tate cancer in a transgenic mouse. Proc Natul Acad Sci USA 1995.. 1 3 .. 1 4 . prostate . seminal vesicle .. progenitor cell androgen dependent stem . 1 3 1 4 . cell .. fetus .. stem cell .. transiently . amplifying cell .. DNA .. embryo .. embryo .. prostate . 97. BCL-2 .. fluctuation . 2 .. PSCA .. GPI .. 98 117.
