Image-J を使用した連続薄切標本からの ... - ndu

1 Copyright 2012 Nippon Dental University, Dept. of Pathology Image-J Image-J JAVA Image-J 1. Image-J OS imagej Windows Mac OS X, Mac OS, Linux PC Windows 64bit OS (32bit 1 2GB ) 2. Image-J Image-J Web download OS Image-J Update Image-J 3.

2 Image-J imagej 3D Viewer StackReg TurboReg Color Deconvolution 4 Image-J piugins 4. Image-J TIFF, GIF, JPEG, DICOM, BMP, PGM, FITS CCD , A001 A002 Cytokeratin /DAB Ki-67 /Vector SG 5.

3 Image-J 1. File Import image Sequence 2. Sequence Option Number of images Starting image : Increment: Scale mages: File name contains: Copyright 2012 Nippon Dental University, Dept. of Pathology Sort names numerically: or enter pattern Convert to RGB Use virtual stack 3. OK 4. 4. 1.

4 image Properties Channels: imagej Slices Frames: Use of Length: nm , um , mm Pixel Width, Pixel Height: Voxel Depth: Frame Interval: / Origin: Global: 2. OK 3. 5 1. Plugins StackReg 2. Transfomation: Translation ( ) Rigid Body ( + ) Scaled Rotation ( + + ) Affine ( + + ) Rigid Body OK Scaled Rotation Affine 4.

5 File Save as image Sequence Copyright 2012 Nippon Dental University, Dept. of Pathology 6. 1. Plugins Color Decomvolution 2. Vectors H&E H DAB DAB + 3. DAB Vector SG From ROI From ROI 3 4. From ROI OK OK DAB DAB Vector SG 3 5.

6 3 6. 3 Binary DAB Vector SG DAB Vector SG 7. File Save as image Sequence 7. 2 1. DAB 2. Process Binary Make Binary DAB 3. Vector SG Vector SG Ki-67 Copyright 2012 Nippon Dental University, Dept. of Pathology 4. 2 File Save as image Sequence 8. Vector SG 2 Ki-67 1.

7 2 Edit Invert 2. / Ki-67 Process image Calculator image 1 image 2 Operation OK 3. File Save as image Sequence Copyright 2012 Nippon Dental University, Dept. of Pathology 9. 1 1. Process Binary Outline 2. File Save as image Sequence 10 outline Ki67 Ki67 1.

8 2. Plugins 3D Viewer imagej 3D Viewer image : Name 3D Viewer Display as: Color: Threshold: Resampling factor: Channels: 3. OK 3 1. imagej 3D Viewer File Add content 3 2. 3 1. imagej 3D Viewer Select 2.

9 imagej 3D Viewer File Edit Attribute Change Color R, G, B 1. imagej 3D Viewer Select 2. imagej 3D Viewer File Edit Attribute Change Transparency Copyright 2012 Nippon Dental University, Dept. of Pathology ON, OFF 1. imagej 3D Viewer Select 2. imagej 3D Viewer File Edit Hide/Show Show Content On/Off imagej 3D Viewer 1. imagej 3D Viewer View Record 360 deg rotation 360 2.

10 imagej File Save as AVI( ) imagej 3D Viewer 3D