Image Lab 中文操作手册 - BIOON

1 800 820 5567 / 400 820 3630 Image Lab 800 820 5567 / 400 820 3630 Image Lab Molecular Imager ChemiDoc XRS+ Molecular Imager Gel Doc XR+ Molecular Imager ChemiDoc MP GS-900 800 820 5567 / 400 820 3630 1. Results Data 2. 3. 1 Image Tools 2 Lane and Band Tools 3 MW Molecular Weight 4 Quantity Tools 5 Annotation Tools 6 Volume Tools Image Lab 1.

2 3D 800 820 5567 / 400 820 3630 Application Blot Chemi (1) Imaging Area (2) Image Exposure Manual Exposure Signal Accumulation Mode SAM ChemiDoc XRS+ SAM 2. (1) Manually set exposure time 10 (2) Highlight saturated pixels (3) (4) (5) 800 820 5567 / 400 820 3630 (6) (7) Image Lab 1994 32 65,535 10 30 300 3.

3 Signal Accumulation Mode SAM SAM Setup 300 5 SAM 250 800 820 5567 / 400 820 3630 Image Transform SAM SAM Creating Protocols 1. -- STEP 1. GEL IMAGING 1 Choose an application 800 820 5567 / 400 820 3630 2 Choose the Imaging Area 3 Choose Image Exposure Time 4 Choose Display Options , Highlight saturated pixels and Image Color 2.

4 Analyze Image -- STEP 2. DETECT LANES AND BANDS 800 820 5567 / 400 820 3630 DETECT LANES AND BANDS Low Band Detection Sensitivity Low sensitivity = 25 High Band Detection Sensitivity High sensitivity = 75 3. Analyze Molecular Weight --STEP 3. ANALYZE MOLECULAR WEIGHT Analyze Molecular Weight Settings Molecular Weight Standard Precision Plus Protein Standards Prestained SDS-PAGE Standards Broad Range Prestained SDS-PAGE Standards Low Range Prestained SDS-PAGE Standards High Range Standard Lanes regression Method 4. Report Settings -- STEP 4. SPECIFY CONTENT OF REPORTS 5. Results Overview Report 800 820 5567 / 400 820 3630 1 Displaying Data 2 Analysis Table Options 3 Lane Lane Profile 800 820 5567 / 400 820 3630 4 Standard Curve Creating Images Analyzing Protocols 1.

5 Analyzing Images 1 Analysis Tool Box A Auto Analysis Detection Settings 800 820 5567 / 400 820 3630 Detect lanes and bands Low Band Detection Sensitivity Low sensitivity = 25 High Band Detection Sensitivity High sensitivity = 75 Molecular Weight Analysis Settings Molecular Weight Standard Standard Lanes regression Method 2. Image Tools Flip 90 Rotate Crop Invert Data Merge 3. Lanes Bands Lane and Band Tools 1 Lane Tab Automatic Manual Lane 800 820 5567 / 400 820 3630 A Lanes All Lanes Resize Adjust Delete B Lane Single Lane Add Bend Move Width Delete C Lane Background Subtraction Apply to selected Lane Lane - Rolling Disk 1-99 mm 2 Bands Tab Detect Bands Bands Add Delete Adjust 4.

6 Molecular Weight Analysis Tools base pairs 5. Quantity Tools 1 Relative Quantity Tab 800 820 5567 / 400 820 3630 reference band R Analysis table 2 Absolute Quantity Tab bands , R bands Linear( ) Point-to-Point cubic spline regression MethodMinimum number of standard bands Minimum number with ForceThrough Option Linear 2 1 Point-to-Point 2 1 cubic spline 5 4 800 820 5567 / 400 820 3630 6. Annotation Tools Add Annotations Alignment 7.

7 Volume Tools Band , Band , Band , kb , ppm , mg/ml .. Rect Tool , , Band C+rl+C+ / 800 820 5567 / 400 820 3630 Band 1 Volume Background Subtraction - Local Local background subtraction unknown volume standard volume pixels - Global Global background subtraction